Abstract
We previously reported that the release of O2 - from isolated pea cell walls was enhanced by a 70-kDa glycoprotein elicitor but was suppressed by mucin-type glycopeptide suppressors (supprescins A and B) prepared from pycnospore germination fluid of Mycosphaerella pinodes, causal agent of Mycosphaerella blight of pea. Here, we show that superoxide dismutase (SOD) in the apoplast fluid/cell wall of pea seedlings responds to the fungal elicitor and suppressor molecules. In a pharmacological study and with internal amino acid sequencing, the apoplastic SOD in a pea cultivar Midoriusui was found to be a Cu/Zn type SOD. We cloned a full-length cDNA of the Cu/Zn-SOD and designated it as PsCu/Zn-SOD1. An increase in PsCu/Zn-SOD1 mRNA and the PsCu/Zn-SOD1 protein was induced by treatment with the elicitor more intensively than by wounding. Such induction by the elicitor or wounding, however, was inhibited by the concomitant presence of supprescins. The SOD activity of recombinant PsCu/Zn-SOD1 was regulated directly by these signal molecules in a manner similar to their effect on the SOD activity in the apoplastic fluid and in the cell wall-bound proteins. Based on these findings, we discuss a role for PsCu/Zn-SOD1 in the pea defense response.
| Original language | English |
|---|---|
| Pages (from-to) | 265-272 |
| Number of pages | 8 |
| Journal | Journal of General Plant Pathology |
| Volume | 72 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Oct 2006 |
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Keywords
- Apoplast
- Cell wall
- Cu/Zn superoxide dismutase (EC 1. 15. 1. 1)
- Defense response
- Mycosphaerella pinodes
- Pisum sativum L.
ASJC Scopus subject areas
- Plant Science
Cite this
Pea extracellular Cu/Zn-superoxide dismutase responsive to signal molecules from a fungal pathogen. / Kasai, Tomonari; Suzuki, Tomoko; Ono, Kozue; Ogawa, Ken'Ichi; Inagaki, Yoshishige; Ichinose, Yuki; Toyoda, Kazuhiro; Shiraishi, Tomonori.
In: Journal of General Plant Pathology, Vol. 72, No. 5, 10.2006, p. 265-272.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Pea extracellular Cu/Zn-superoxide dismutase responsive to signal molecules from a fungal pathogen
AU - Kasai, Tomonari
AU - Suzuki, Tomoko
AU - Ono, Kozue
AU - Ogawa, Ken'Ichi
AU - Inagaki, Yoshishige
AU - Ichinose, Yuki
AU - Toyoda, Kazuhiro
AU - Shiraishi, Tomonori
PY - 2006/10
Y1 - 2006/10
N2 - We previously reported that the release of O2 - from isolated pea cell walls was enhanced by a 70-kDa glycoprotein elicitor but was suppressed by mucin-type glycopeptide suppressors (supprescins A and B) prepared from pycnospore germination fluid of Mycosphaerella pinodes, causal agent of Mycosphaerella blight of pea. Here, we show that superoxide dismutase (SOD) in the apoplast fluid/cell wall of pea seedlings responds to the fungal elicitor and suppressor molecules. In a pharmacological study and with internal amino acid sequencing, the apoplastic SOD in a pea cultivar Midoriusui was found to be a Cu/Zn type SOD. We cloned a full-length cDNA of the Cu/Zn-SOD and designated it as PsCu/Zn-SOD1. An increase in PsCu/Zn-SOD1 mRNA and the PsCu/Zn-SOD1 protein was induced by treatment with the elicitor more intensively than by wounding. Such induction by the elicitor or wounding, however, was inhibited by the concomitant presence of supprescins. The SOD activity of recombinant PsCu/Zn-SOD1 was regulated directly by these signal molecules in a manner similar to their effect on the SOD activity in the apoplastic fluid and in the cell wall-bound proteins. Based on these findings, we discuss a role for PsCu/Zn-SOD1 in the pea defense response.
AB - We previously reported that the release of O2 - from isolated pea cell walls was enhanced by a 70-kDa glycoprotein elicitor but was suppressed by mucin-type glycopeptide suppressors (supprescins A and B) prepared from pycnospore germination fluid of Mycosphaerella pinodes, causal agent of Mycosphaerella blight of pea. Here, we show that superoxide dismutase (SOD) in the apoplast fluid/cell wall of pea seedlings responds to the fungal elicitor and suppressor molecules. In a pharmacological study and with internal amino acid sequencing, the apoplastic SOD in a pea cultivar Midoriusui was found to be a Cu/Zn type SOD. We cloned a full-length cDNA of the Cu/Zn-SOD and designated it as PsCu/Zn-SOD1. An increase in PsCu/Zn-SOD1 mRNA and the PsCu/Zn-SOD1 protein was induced by treatment with the elicitor more intensively than by wounding. Such induction by the elicitor or wounding, however, was inhibited by the concomitant presence of supprescins. The SOD activity of recombinant PsCu/Zn-SOD1 was regulated directly by these signal molecules in a manner similar to their effect on the SOD activity in the apoplastic fluid and in the cell wall-bound proteins. Based on these findings, we discuss a role for PsCu/Zn-SOD1 in the pea defense response.
KW - Apoplast
KW - Cell wall
KW - Cu/Zn superoxide dismutase (EC 1. 15. 1. 1)
KW - Defense response
KW - Mycosphaerella pinodes
KW - Pisum sativum L.
UR - http://www.scopus.com/inward/record.url?scp=33749352251&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33749352251&partnerID=8YFLogxK
U2 - 10.1007/s10327-006-0283-y
DO - 10.1007/s10327-006-0283-y
M3 - Article
AN - SCOPUS:33749352251
VL - 72
SP - 265
EP - 272
JO - Journal of General Plant Pathology
JF - Journal of General Plant Pathology
SN - 1345-2630
IS - 5
ER -