Pea extracellular Cu/Zn-superoxide dismutase responsive to signal molecules from a fungal pathogen

Tomonari Kasai, Tomoko Suzuki, Kozue Ono, Ken'Ichi Ogawa, Yoshishige Inagaki, Yuki Ichinose, Kazuhiro Toyoda, Tomonori Shiraishi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We previously reported that the release of O2 - from isolated pea cell walls was enhanced by a 70-kDa glycoprotein elicitor but was suppressed by mucin-type glycopeptide suppressors (supprescins A and B) prepared from pycnospore germination fluid of Mycosphaerella pinodes, causal agent of Mycosphaerella blight of pea. Here, we show that superoxide dismutase (SOD) in the apoplast fluid/cell wall of pea seedlings responds to the fungal elicitor and suppressor molecules. In a pharmacological study and with internal amino acid sequencing, the apoplastic SOD in a pea cultivar Midoriusui was found to be a Cu/Zn type SOD. We cloned a full-length cDNA of the Cu/Zn-SOD and designated it as PsCu/Zn-SOD1. An increase in PsCu/Zn-SOD1 mRNA and the PsCu/Zn-SOD1 protein was induced by treatment with the elicitor more intensively than by wounding. Such induction by the elicitor or wounding, however, was inhibited by the concomitant presence of supprescins. The SOD activity of recombinant PsCu/Zn-SOD1 was regulated directly by these signal molecules in a manner similar to their effect on the SOD activity in the apoplastic fluid and in the cell wall-bound proteins. Based on these findings, we discuss a role for PsCu/Zn-SOD1 in the pea defense response.

Original languageEnglish
Pages (from-to)265-272
Number of pages8
JournalJournal of General Plant Pathology
Volume72
Issue number5
DOIs
Publication statusPublished - Oct 2006

Fingerprint

peas
superoxide dismutase
pathogens
cell walls
Mycosphaerella pinodes
glycopeptides
Mycosphaerella
mucins
apoplast
blight
glycoproteins
proteins
germination
amino acids
seedlings
elicitors
cultivars
fluids

Keywords

  • Apoplast
  • Cell wall
  • Cu/Zn superoxide dismutase (EC 1. 15. 1. 1)
  • Defense response
  • Mycosphaerella pinodes
  • Pisum sativum L.

ASJC Scopus subject areas

  • Plant Science

Cite this

Pea extracellular Cu/Zn-superoxide dismutase responsive to signal molecules from a fungal pathogen. / Kasai, Tomonari; Suzuki, Tomoko; Ono, Kozue; Ogawa, Ken'Ichi; Inagaki, Yoshishige; Ichinose, Yuki; Toyoda, Kazuhiro; Shiraishi, Tomonori.

In: Journal of General Plant Pathology, Vol. 72, No. 5, 10.2006, p. 265-272.

Research output: Contribution to journalArticle

Kasai, Tomonari ; Suzuki, Tomoko ; Ono, Kozue ; Ogawa, Ken'Ichi ; Inagaki, Yoshishige ; Ichinose, Yuki ; Toyoda, Kazuhiro ; Shiraishi, Tomonori. / Pea extracellular Cu/Zn-superoxide dismutase responsive to signal molecules from a fungal pathogen. In: Journal of General Plant Pathology. 2006 ; Vol. 72, No. 5. pp. 265-272.
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