Pdk1 kinase regulates basal disease resistance through the OsOxi1-OsPti1a phosphorylation cascade in rice

Hidenori Matsui, Akio Miyao, Akira Takahashi, Hirohiko Hirochika

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The AGC kinase OsOxi1, which has been isolated as an interactor with OsPti1a, positively regulates basal disease resistance in rice. In eukaryotes, AGC kinase family proteins are regulated by 3-phosphoinositide-dependent protein kinase 1 (Pdk1). In Arabidopsis, AtPdk1 directly interacts with phosphatidic acid, which functions as a second messenger in both biotic and abiotic stress responses. However, the functions of Pdk1 are poorly understood in plants. We show here that OsPdk1 acts upstream of the OsOxi1-OsPti1a signal cascade in disease resistance in rice. OsPdk1 interacts with OsOxi1 and phosphorylates the Ser283 residue of OsOxi1 in vitro. To investigate whether OsPdk1 is involved in immunity that is triggered by microbial-associated molecular patterns, we analyzed the phosphorylation status of OsPdk1 in response to chitin elicitor. Like OsOxi1, OsPdk1 is rapidly phosphorylated in response to chitin elicitor, suggesting that OsPdk1 participates in signal transduction through pathogen recognition. The overexpression of OsPdk1 enhanced basal resistance against a blast fungus, Magnaporthe oryzae, and a bacterial pathogen, Xanthomonas oryzae pv. oryzae (Xoo). Taken together, these results suggest that OsPdk1 positively regulates basal disease resistance through the OsOxi1-OsPti1a phosphorylation cascade in rice.

Original languageEnglish
Pages (from-to)2082-2091
Number of pages10
JournalPlant and Cell Physiology
Volume51
Issue number12
DOIs
Publication statusPublished - Dec 2010
Externally publishedYes

Fingerprint

Disease Resistance
Phosphatidylinositols
protein kinases
Protein Kinases
disease resistance
phosphorylation
phosphotransferases (kinases)
Phosphotransferases
Phosphorylation
chitin
rice
Xanthomonas oryzae pv. oryzae
Magnaporthe oryzae
Chitin
pathogens
second messengers
biotic stress
abiotic stress
eukaryotic cells
signal transduction

Keywords

  • 3-phosphoinositide-dependent protein kinase
  • AGC kinase
  • Chitin elicitor
  • Disease resistance
  • Pto-interacting protein
  • Rice

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Pdk1 kinase regulates basal disease resistance through the OsOxi1-OsPti1a phosphorylation cascade in rice. / Matsui, Hidenori; Miyao, Akio; Takahashi, Akira; Hirochika, Hirohiko.

In: Plant and Cell Physiology, Vol. 51, No. 12, 12.2010, p. 2082-2091.

Research output: Contribution to journalArticle

Matsui, Hidenori ; Miyao, Akio ; Takahashi, Akira ; Hirochika, Hirohiko. / Pdk1 kinase regulates basal disease resistance through the OsOxi1-OsPti1a phosphorylation cascade in rice. In: Plant and Cell Physiology. 2010 ; Vol. 51, No. 12. pp. 2082-2091.
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