PCR sexing and developmental rate differences in preimplantation mouse embryos fertilized and cultured in vitro

Ruby P.A. Valdivia, Tetsuo Kunieda, Sadahiro Azuma, Yutaka Toyoda

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81 Citations (Scopus)

Abstract

A two‐step polymerase chain reaction (PCR) assay was used to determine the sex of mouse preimplantation embryos obtained from oocytes fertilized and cultured in vitro, to investigate the differences in the developmental rates of mouse embryos according to the sex. All the in vitro developed embryos could be analyzed by this method. When the embryos were classified according to the time of morula to blastocyst transition as fast‐intermediate‐ and slow‐growing embryos, a significantly high percentage (78.0%) of the fast‐developing embryos were identified as males; while a significantly lower percentage (42.5%) of slow‐developing embryos were identified as males. The intermediate‐developing embryos presented a sex ratio not significantly different from the total (57.5%). The deviation of sex ratio was further confirmed by embryo transfer experiment, where fast‐ and slow‐developing embryos gave 76.2% and 25.7% male fetuses, respectively. We concluded that male mouse embryos fertilized and cultured in vitro develop faster than female embryos. © 1993 Wiley‐Liss, Inc.

Original languageEnglish
Pages (from-to)121-126
Number of pages6
JournalMolecular Reproduction and Development
Volume35
Issue number2
DOIs
Publication statusPublished - Jun 1993

Keywords

  • Blastocyst
  • Sex ratio
  • Zfy

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

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