PCR sexing and developmental rate differences in preimplantation mouse embryos fertilized and cultured in vitro

R. P A Valdivia, Tetsuo Kunieda, S. Azuma, Y. Toyoda

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

A two-step polymerase chain reaction (PCR) assay was used to determine the sex of mouse preimplantation embryos obtained from oocytes fertilized and cultured in vitro, to investigate the differences in the developmental rates of mouse embryos according to the sex. All the in vitro developed embryos could be analyzed by this method. When the embryos were classified according to the time of morula to blastocyst transition as fast-intermediate- and slow-growing embryos, a significantly high percentage (78.0%) of the fast- developing embryos were identified as males; while a significantly lower percentage (42.5%) of slow-developing embryos were identified as males. The intermediate-developing embryos presented a sex ratio not significantly different from the total (57.5%). The deviation of sex ratio was further confirmed by embryo transfer experiment, where fast- and slow-developing embryos gave 76.2% and 25.7% male fetuses, respectively. We concluded that male mouse embryos fertilized and cultured in vitro develop faster than female embryos.

Original languageEnglish
Pages (from-to)121-126
Number of pages6
JournalMolecular Reproduction and Development
Volume35
Issue number2
DOIs
Publication statusPublished - 1993
Externally publishedYes

Fingerprint

Blastocyst
Embryonic Structures
Polymerase Chain Reaction
Sex Ratio
In Vitro Techniques
Morula
Embryo Transfer
Oocytes
Fetus

Keywords

  • Blastocyst
  • Sex ratio
  • Zfy

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

PCR sexing and developmental rate differences in preimplantation mouse embryos fertilized and cultured in vitro. / Valdivia, R. P A; Kunieda, Tetsuo; Azuma, S.; Toyoda, Y.

In: Molecular Reproduction and Development, Vol. 35, No. 2, 1993, p. 121-126.

Research output: Contribution to journalArticle

@article{88d873c2ab0244a88d2183104926823b,
title = "PCR sexing and developmental rate differences in preimplantation mouse embryos fertilized and cultured in vitro",
abstract = "A two-step polymerase chain reaction (PCR) assay was used to determine the sex of mouse preimplantation embryos obtained from oocytes fertilized and cultured in vitro, to investigate the differences in the developmental rates of mouse embryos according to the sex. All the in vitro developed embryos could be analyzed by this method. When the embryos were classified according to the time of morula to blastocyst transition as fast-intermediate- and slow-growing embryos, a significantly high percentage (78.0{\%}) of the fast- developing embryos were identified as males; while a significantly lower percentage (42.5{\%}) of slow-developing embryos were identified as males. The intermediate-developing embryos presented a sex ratio not significantly different from the total (57.5{\%}). The deviation of sex ratio was further confirmed by embryo transfer experiment, where fast- and slow-developing embryos gave 76.2{\%} and 25.7{\%} male fetuses, respectively. We concluded that male mouse embryos fertilized and cultured in vitro develop faster than female embryos.",
keywords = "Blastocyst, Sex ratio, Zfy",
author = "Valdivia, {R. P A} and Tetsuo Kunieda and S. Azuma and Y. Toyoda",
year = "1993",
doi = "10.1002/mrd.1080350204",
language = "English",
volume = "35",
pages = "121--126",
journal = "Molecular Reproduction and Development",
issn = "1040-452X",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - PCR sexing and developmental rate differences in preimplantation mouse embryos fertilized and cultured in vitro

AU - Valdivia, R. P A

AU - Kunieda, Tetsuo

AU - Azuma, S.

AU - Toyoda, Y.

PY - 1993

Y1 - 1993

N2 - A two-step polymerase chain reaction (PCR) assay was used to determine the sex of mouse preimplantation embryos obtained from oocytes fertilized and cultured in vitro, to investigate the differences in the developmental rates of mouse embryos according to the sex. All the in vitro developed embryos could be analyzed by this method. When the embryos were classified according to the time of morula to blastocyst transition as fast-intermediate- and slow-growing embryos, a significantly high percentage (78.0%) of the fast- developing embryos were identified as males; while a significantly lower percentage (42.5%) of slow-developing embryos were identified as males. The intermediate-developing embryos presented a sex ratio not significantly different from the total (57.5%). The deviation of sex ratio was further confirmed by embryo transfer experiment, where fast- and slow-developing embryos gave 76.2% and 25.7% male fetuses, respectively. We concluded that male mouse embryos fertilized and cultured in vitro develop faster than female embryos.

AB - A two-step polymerase chain reaction (PCR) assay was used to determine the sex of mouse preimplantation embryos obtained from oocytes fertilized and cultured in vitro, to investigate the differences in the developmental rates of mouse embryos according to the sex. All the in vitro developed embryos could be analyzed by this method. When the embryos were classified according to the time of morula to blastocyst transition as fast-intermediate- and slow-growing embryos, a significantly high percentage (78.0%) of the fast- developing embryos were identified as males; while a significantly lower percentage (42.5%) of slow-developing embryos were identified as males. The intermediate-developing embryos presented a sex ratio not significantly different from the total (57.5%). The deviation of sex ratio was further confirmed by embryo transfer experiment, where fast- and slow-developing embryos gave 76.2% and 25.7% male fetuses, respectively. We concluded that male mouse embryos fertilized and cultured in vitro develop faster than female embryos.

KW - Blastocyst

KW - Sex ratio

KW - Zfy

UR - http://www.scopus.com/inward/record.url?scp=0027191877&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027191877&partnerID=8YFLogxK

U2 - 10.1002/mrd.1080350204

DO - 10.1002/mrd.1080350204

M3 - Article

VL - 35

SP - 121

EP - 126

JO - Molecular Reproduction and Development

JF - Molecular Reproduction and Development

SN - 1040-452X

IS - 2

ER -