PCR-dipstick chromatography for differential detection of carbapenemase genes directly in stool specimens

Rathina Kumar Shanmugakani, Yukihiro Akeda, Norihisa Yamamoto, Noriko Sakamoto, Hideharu Hagiya, Hisao Yoshida, Dan Takeuchi, Yo Sugawara, Takuya Kodera, Mitsuo Kawase, Warawut Laolerd, Narong Chaihongsa, Pitak Santanirand, Yoshikazu Ishii, Shigeyuki Hamada, Kazunori Tomono

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A PCR-dipstick chromatography technique was designed and evaluated for differential identification of blaNDM, blaKPC, blaIMP, and blaOXA-48 carbapenemase genes directly in stool specimens within 2 h. It is a DNA-DNA hybridization-based detection system where PCR products can be easily interpreted by visual observation without electrophoresis. The PCR-dipstick showed high sensitivity (93.3%) and specificity (99.1%) in directly detecting carbapenemase genes in stool specimens compared with multiplex PCR for genomic DNA of the isolates from those stool specimens.

Original languageEnglish
Article numbere00067-17
JournalAntimicrobial Agents and Chemotherapy
Volume61
Issue number6
DOIs
Publication statusPublished - Jun 2017

Keywords

  • Carbapenemase
  • Enterobacteriaceae
  • Molecular diagnostics

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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    Shanmugakani, R. K., Akeda, Y., Yamamoto, N., Sakamoto, N., Hagiya, H., Yoshida, H., Takeuchi, D., Sugawara, Y., Kodera, T., Kawase, M., Laolerd, W., Chaihongsa, N., Santanirand, P., Ishii, Y., Hamada, S., & Tomono, K. (2017). PCR-dipstick chromatography for differential detection of carbapenemase genes directly in stool specimens. Antimicrobial Agents and Chemotherapy, 61(6), [e00067-17]. https://doi.org/10.1128/AAC.00067-17