Pc3-secreted microprotein is expressed in glioblastoma stem-like cells and human glioma tissues

Masato Maruyama, Yousuke Nakano, Takuya Nishimura, Ryoichi Iwata, Satoshi Matsuda, Mikio Hayashi, Yuki Nakai, Masahiro Nonaka, Tetsuo Sugimoto

Research output: Contribution to journalArticlepeer-review

Abstract

Glioblastoma multiforme (GBM) is the most prevalent malignant primary brain tumor with a high recurrence rate. Despite multimodal therapy including surgical resection, chemotherapy, and radiotherapy, the median survival time after the initial diagnosis of GBM is approximately 14 months. Since cancer stem cells (CSCs) are considered the leading cause of cancer recurrence, glioblastoma stem cell-targeted therapy is a promising strategy for the treatment of GBM. However, because CSC heterogeneity has been implicated in the difficulties of CSC-target therapy, more in-depth knowledge of CSC biology is still required to develop novel therapies. In this study, we established single cell-derived tumorspheres from human glioblastoma U87MG cells. One of these tumorspheres, P4E8 clone, showed CSC-like phenotypes, such as self-renewal capacity, expression of CSC markers, resistance to anti-cancer agents, and in vivo tumorigenicity. Therefore, we used P4E8 cells as a cell-based model of glioblastoma stem cells (GSCs). Gene expression analysis using microarray indicated that the most highly expressed genes in P4E8 cells compared to the parental U87MG were PC3-secreted microprotein (MSMP). Furthermore, MSMP was expressed in patient-derived GSCs and human glioma tissues at the protein level, implying that MSMP might contribute to glioma development and progression.

Original languageEnglish
Pages (from-to)910-919
Number of pages10
JournalBiological and Pharmaceutical Bulletin
Volume44
Issue number7
DOIs
Publication statusPublished - Jul 2021

Keywords

  • Cancer stem-like cell
  • DNA microarray
  • Glioblastoma
  • PC3-secreted microprotein
  • Tumorsphere

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

Fingerprint

Dive into the research topics of 'Pc3-secreted microprotein is expressed in glioblastoma stem-like cells and human glioma tissues'. Together they form a unique fingerprint.

Cite this