Abstract
Aim: Soft-tissue sarcomas are a group of rare mesenchymal carcinomas that include approximately 50 histological types, and account for 1% of all adult cancer cases. The yearly incidence of soft-tissue sarcomas in the USA is approximately 11,280 cases, with an overall mortality of 3,900 deaths per year. Materials and Methods: In this study, we established a patient-derived orthotopic xenograft (PDOX) from a patient with a soft-tissue sarcoma of the retroperitoneum in nude mice and compared it to a subcutaneous patient-derived model of the same tumor for histology. Results: In the PDOX model, a bulky tumor grew in the left retroperitoneum in the same manner as the patient's tumor. Upon histological examination, the majority of the PDOX tissue section comprised sarcomatous highgrade spindle cells of varying sizes, similar to the original patient tumor. In contrast, the majority of the subcutaneously-implanted tumor comprised round to oval cells. Conclusion: These results indicate that the PDOX recapitulated the histology of the original tumor more than the subcutaneous model.
| Original language | English |
|---|---|
| Pages (from-to) | 697-701 |
| Number of pages | 5 |
| Journal | Anticancer Research |
| Volume | 35 |
| Issue number | 2 |
| Publication status | Published - Jan 1 2015 |
| Externally published | Yes |
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Keywords
- Histology
- Nude mouse
- Patient-derived orthotopic xenograft (PDOX)
- Soft-tissue sarcoma
- Spindle cells
- Subcutaneous model
ASJC Scopus subject areas
- Oncology
- Cancer Research
Cite this
Patient-derived orthotopic xenograft (PDOX) nude mouse model of soft-tissue sarcoma more closely mimics the patient behavior in contrast to the subcutaneous ectopic model. / Hiroshima, Yukihiko; Zhang, Yong; Zhang, Nan; Uehara, Fuminari; Maawy, Ali; Murakami, Takashi; Mii, Sumiyuki; Yamamoto, Mako; Miwa, Shinji; Yano, Shuuya; Momiyama, Masashi; Mori, Ryutaro; Matsuyama, Ryusei; Chishima, Takashi; Tanaka, Kuniya; Ichikawa, Yasushi; Bouvet, Michael; Endo, Itaru; Hoffman, Robert M.
In: Anticancer Research, Vol. 35, No. 2, 01.01.2015, p. 697-701.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Patient-derived orthotopic xenograft (PDOX) nude mouse model of soft-tissue sarcoma more closely mimics the patient behavior in contrast to the subcutaneous ectopic model
AU - Hiroshima, Yukihiko
AU - Zhang, Yong
AU - Zhang, Nan
AU - Uehara, Fuminari
AU - Maawy, Ali
AU - Murakami, Takashi
AU - Mii, Sumiyuki
AU - Yamamoto, Mako
AU - Miwa, Shinji
AU - Yano, Shuuya
AU - Momiyama, Masashi
AU - Mori, Ryutaro
AU - Matsuyama, Ryusei
AU - Chishima, Takashi
AU - Tanaka, Kuniya
AU - Ichikawa, Yasushi
AU - Bouvet, Michael
AU - Endo, Itaru
AU - Hoffman, Robert M.
PY - 2015/1/1
Y1 - 2015/1/1
N2 - Aim: Soft-tissue sarcomas are a group of rare mesenchymal carcinomas that include approximately 50 histological types, and account for 1% of all adult cancer cases. The yearly incidence of soft-tissue sarcomas in the USA is approximately 11,280 cases, with an overall mortality of 3,900 deaths per year. Materials and Methods: In this study, we established a patient-derived orthotopic xenograft (PDOX) from a patient with a soft-tissue sarcoma of the retroperitoneum in nude mice and compared it to a subcutaneous patient-derived model of the same tumor for histology. Results: In the PDOX model, a bulky tumor grew in the left retroperitoneum in the same manner as the patient's tumor. Upon histological examination, the majority of the PDOX tissue section comprised sarcomatous highgrade spindle cells of varying sizes, similar to the original patient tumor. In contrast, the majority of the subcutaneously-implanted tumor comprised round to oval cells. Conclusion: These results indicate that the PDOX recapitulated the histology of the original tumor more than the subcutaneous model.
AB - Aim: Soft-tissue sarcomas are a group of rare mesenchymal carcinomas that include approximately 50 histological types, and account for 1% of all adult cancer cases. The yearly incidence of soft-tissue sarcomas in the USA is approximately 11,280 cases, with an overall mortality of 3,900 deaths per year. Materials and Methods: In this study, we established a patient-derived orthotopic xenograft (PDOX) from a patient with a soft-tissue sarcoma of the retroperitoneum in nude mice and compared it to a subcutaneous patient-derived model of the same tumor for histology. Results: In the PDOX model, a bulky tumor grew in the left retroperitoneum in the same manner as the patient's tumor. Upon histological examination, the majority of the PDOX tissue section comprised sarcomatous highgrade spindle cells of varying sizes, similar to the original patient tumor. In contrast, the majority of the subcutaneously-implanted tumor comprised round to oval cells. Conclusion: These results indicate that the PDOX recapitulated the histology of the original tumor more than the subcutaneous model.
KW - Histology
KW - Nude mouse
KW - Patient-derived orthotopic xenograft (PDOX)
KW - Soft-tissue sarcoma
KW - Spindle cells
KW - Subcutaneous model
UR - http://www.scopus.com/inward/record.url?scp=84923666782&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84923666782&partnerID=8YFLogxK
M3 - Article
C2 - 25667448
AN - SCOPUS:84923666782
VL - 35
SP - 697
EP - 701
JO - Anticancer Research
JF - Anticancer Research
SN - 0250-7005
IS - 2
ER -