Patient-derived orthotopic xenograft (PDOX) nude mouse model of soft-tissue sarcoma more closely mimics the patient behavior in contrast to the subcutaneous ectopic model

Yukihiko Hiroshima, Yong Zhang, Nan Zhang, Fuminari Uehara, Ali Maawy, Takashi Murakami, Sumiyuki Mii, Mako Yamamoto, Shinji Miwa, Shuya Yano, Masashi Momiyama, Ryutaro Mori, Ryusei Matsuyama, Takashi Chishima, Kuniya Tanaka, Yasushi Ichikawa, Michael Bouvet, Itaru Endo, Robert M. Hoffman

Research output: Contribution to journalArticlepeer-review

65 Citations (Scopus)

Abstract

Aim: Soft-tissue sarcomas are a group of rare mesenchymal carcinomas that include approximately 50 histological types, and account for 1% of all adult cancer cases. The yearly incidence of soft-tissue sarcomas in the USA is approximately 11,280 cases, with an overall mortality of 3,900 deaths per year. Materials and Methods: In this study, we established a patient-derived orthotopic xenograft (PDOX) from a patient with a soft-tissue sarcoma of the retroperitoneum in nude mice and compared it to a subcutaneous patient-derived model of the same tumor for histology. Results: In the PDOX model, a bulky tumor grew in the left retroperitoneum in the same manner as the patient's tumor. Upon histological examination, the majority of the PDOX tissue section comprised sarcomatous highgrade spindle cells of varying sizes, similar to the original patient tumor. In contrast, the majority of the subcutaneously-implanted tumor comprised round to oval cells. Conclusion: These results indicate that the PDOX recapitulated the histology of the original tumor more than the subcutaneous model.

Original languageEnglish
Pages (from-to)697-701
Number of pages5
JournalAnticancer research
Volume35
Issue number2
Publication statusPublished - Feb 1 2015
Externally publishedYes

Keywords

  • Histology
  • Nude mouse
  • Patient-derived orthotopic xenograft (PDOX)
  • Soft-tissue sarcoma
  • Spindle cells
  • Subcutaneous model

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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