Pathological and molecular diagnosis of the 2013 African swine fever outbreak in Lusaka, Zambia

John Yabe, Pharaoh Hamambulu, Edgar Simulundu, Hirohito Ogawa, Masahiro Kajihara, Akina Mori-Kajihara, Katendi Changula-Chitanga, Max Mwase, Mutinta Mweemba-Muwowo, Herman Moses Chambaro, Liywalii Mataa, Bernard Hang’ombe, Bonniface Namangala, Paul Fandamu, Hirofumi Sawa, Ayato Takada, Hideaki Higashi, Aaron Simanyengwe Mweene

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


African swine fever (ASF) is a highly contagious and fatal hemorrhagic viral disease of domestic pigs. The disease is widespread in sub-Saharan Africa and has repeatedly been introduced into other continents. The current study describes the diagnostic investigations of a hemorrhagic disease that was reported in pigs in Lusaka (October 2013), Zambia. Necropsy, histopathology, and molecular diagnosis using polymerase chain reaction and sequence analysis confirmed the disease to be ASF. The sequences obtained showed high similarity to previously isolated ASF viruses. Consistent surveillance and rapid diagnosis of the disease is recommended to prevent future outbreaks and economic losses as there is currently no vaccine against the disease.

Original languageEnglish
Pages (from-to)459-463
Number of pages5
JournalTropical Animal Health and Production
Issue number2
Publication statusPublished - Feb 2014
Externally publishedYes


  • African swine fever
  • PCR
  • Pathology
  • Zambia

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology


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