Pathogenic mechanisms of influenza A(H1N1)pdm09 infection elucidated on gene expression profiling

Nobuko Yamashita, Hirokazu Tsukahara, Mitsuru Tsuge, Yoshiharu Nagaoka, Masato Yashiro, Yukie Saito, Yosuke Fujii, Takashi Oka, Tsuneo Morishima

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: The pathogenic mechanisms underlying influenza A(H1N1)pdm09-associated central nervous system (CNS) manifestations and pneumonia remain unclear. This study examined A(H1N1)pdm09 host responses using gene expression profiles of patients' peripheral blood. Methods: Sixteen A(H1N1)pdm09-infected children in three groups were examined: a CNS group, with convulsion and altered consciousness (n = 6); a pneumonia (Pneu) group (n = 5); and a group of infected control patients (n = 5). The signal ratios of the acute to recovery phases in CNS or Pneu were analyzed versus those of the control. Results: The CNS (619 transcripts) and Pneu (656 transcripts) groups had significantly increased signal ratios compared to the control group. Regarding the increased ratios of transcripts shown by multiple probes, contactin-associated protein-like 3 transcripts, oleoyl-ACP hydrolase transcripts, and interleukin 1 type 1 receptor were observed in CNS and Pneu. Increased ratios of prostaglandin-endoperoxide synthase 2 and α-synuclein were characteristic of CNS. Alkaline phosphatase and the Fc fragment of IgA receptor were characteristic of Pneu. Regarding enriched gene ontology terms, 'response to lipopolysaccharide', 'innate immune response', and 'intrinsic to membrane' were observed commonly in CNS and Pneu. Enriched gene ontology terms related to 'hemoglobin' and 'hemostasis' were, respectively, characteristic of CNS and Pneu. Conclusion: These symptom-associated transcripts might be some clues to the pathogenesis of the A(H1N1)pdm09 infection.

Original languageEnglish
Pages (from-to)572-577
Number of pages6
JournalPediatrics International
Volume55
Issue number5
DOIs
Publication statusPublished - Oct 2013

Fingerprint

Gene Expression Profiling
Human Influenza
Pneumonia
Central Nervous System
Infection
Gene Ontology
Contactins
Interleukin-1 Type I Receptors
Synucleins
Immunoglobulin Fc Fragments
Control Groups
Hydrolases
Prostaglandin-Endoperoxide Synthases
Hemostasis
Consciousness
Transcriptome
Innate Immunity
Alkaline Phosphatase
Lipopolysaccharides
Hemoglobins

Keywords

  • central nervous system manifestations
  • gene expression profile
  • influenza A(H1N1)pdm09
  • pneumonia

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Pathogenic mechanisms of influenza A(H1N1)pdm09 infection elucidated on gene expression profiling. / Yamashita, Nobuko; Tsukahara, Hirokazu; Tsuge, Mitsuru; Nagaoka, Yoshiharu; Yashiro, Masato; Saito, Yukie; Fujii, Yosuke; Oka, Takashi; Morishima, Tsuneo.

In: Pediatrics International, Vol. 55, No. 5, 10.2013, p. 572-577.

Research output: Contribution to journalArticle

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