Pathogenic link between hydroa vacciniforme and Epstein-Barr virus-associated hematologic disorders

Keiji Iwatsuki, Masataka Satoh, Takenobu Yamamoto, Takashi Oono, Shin Morizane, Mikio Ohtsuka, Zi Gang Xu, Daisuke Suzuki, Kazuhide Tsuji

Research output: Contribution to journalArticle

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Abstract

Objectives: To determine the pathogenic association of latent Epstein-Barr virus (EBV) infections with both typical hydroa vacciniforme (HV) and severe HV-like eruptions, and to survey the complications and outcomes of patients. Design: Case series. Patients: Twenty-nine patients with HV or severe HV-like eruptions. Interventions: In situ hybridization and immunostaining of biopsy specimens; extraction of DNA samples from cutaneous lesions and/or peripheral blood mononuclear cells for EBV DNA assay. Main Outcome Measures: Clinicopathologic manifestations, hematologic findings, complications, and outcomes; presence of latent EBV infection. Results: T cells positive for EBV-encoded small nuclear RNA (EBER) were detected, to various degrees, in cutaneous infiltrates in 28 (97%) of 29 patients, including all 6 patients with definite HV with a positive phototest reaction, 11 of 12 patients with probable HV without evidence of photosensitivity, and all 11 patients with severe HV associated with systemic symptoms. In addition to EBER-positive T cells, many cytotoxic T lymphocytes expressing T-cell intracellular antigen 1 and granzyme B were present in the cutaneous lesions. Natural killer (NK) cells were absent or at a background level. The UV-induced cutaneous lesions showed histopathologic findings consistent with those of HV, containing many EBER-positive cells. Although no hematologic abnormalities were found in the definite and probable HV groups, the amounts of EBV DNA were increased in the peripheral blood mononuclear cells. By contrast, the severe HV group had markedly increased levels of EBV DNA associated with NK-cell lymphocytosis, and complications including chronic active EBV infection, hypersensitivity to mosquito bites, and hemophagocytic syndrome. Five patients with severe disease died of EBV-associated NK/T-cell lymphomas or hemophagocytic syndrome 2 to 14 years after onset. Conclusion: Both typical and severe HV are included within the spectrum of cutaneous disorders mediated by EBV-infected T cells, and the severe HV group may have overt EBV-associated NK/T-cell lymphoproliferative disorders with a frequently fatal outcome.

Original languageEnglish
Pages (from-to)587-595
Number of pages9
JournalArchives of Dermatology
Volume142
Issue number5
DOIs
Publication statusPublished - May 2006

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Hydroa Vacciniforme
Human Herpesvirus 4
Small Nuclear RNA
Epstein-Barr Virus Infections
Skin
T-Lymphocytes
Hemophagocytic Lymphohistiocytosis
Natural Killer T-Cells
DNA
Natural Killer Cells
Blood Cells
Granzymes
Lymphocytosis
Fatal Outcome
Lymphoproliferative Disorders
T-Cell Lymphoma
Cytotoxic T-Lymphocytes
Bites and Stings

ASJC Scopus subject areas

  • Dermatology

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Pathogenic link between hydroa vacciniforme and Epstein-Barr virus-associated hematologic disorders. / Iwatsuki, Keiji; Satoh, Masataka; Yamamoto, Takenobu; Oono, Takashi; Morizane, Shin; Ohtsuka, Mikio; Xu, Zi Gang; Suzuki, Daisuke; Tsuji, Kazuhide.

In: Archives of Dermatology, Vol. 142, No. 5, 05.2006, p. 587-595.

Research output: Contribution to journalArticle

Iwatsuki, Keiji ; Satoh, Masataka ; Yamamoto, Takenobu ; Oono, Takashi ; Morizane, Shin ; Ohtsuka, Mikio ; Xu, Zi Gang ; Suzuki, Daisuke ; Tsuji, Kazuhide. / Pathogenic link between hydroa vacciniforme and Epstein-Barr virus-associated hematologic disorders. In: Archives of Dermatology. 2006 ; Vol. 142, No. 5. pp. 587-595.
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abstract = "Objectives: To determine the pathogenic association of latent Epstein-Barr virus (EBV) infections with both typical hydroa vacciniforme (HV) and severe HV-like eruptions, and to survey the complications and outcomes of patients. Design: Case series. Patients: Twenty-nine patients with HV or severe HV-like eruptions. Interventions: In situ hybridization and immunostaining of biopsy specimens; extraction of DNA samples from cutaneous lesions and/or peripheral blood mononuclear cells for EBV DNA assay. Main Outcome Measures: Clinicopathologic manifestations, hematologic findings, complications, and outcomes; presence of latent EBV infection. Results: T cells positive for EBV-encoded small nuclear RNA (EBER) were detected, to various degrees, in cutaneous infiltrates in 28 (97{\%}) of 29 patients, including all 6 patients with definite HV with a positive phototest reaction, 11 of 12 patients with probable HV without evidence of photosensitivity, and all 11 patients with severe HV associated with systemic symptoms. In addition to EBER-positive T cells, many cytotoxic T lymphocytes expressing T-cell intracellular antigen 1 and granzyme B were present in the cutaneous lesions. Natural killer (NK) cells were absent or at a background level. The UV-induced cutaneous lesions showed histopathologic findings consistent with those of HV, containing many EBER-positive cells. Although no hematologic abnormalities were found in the definite and probable HV groups, the amounts of EBV DNA were increased in the peripheral blood mononuclear cells. By contrast, the severe HV group had markedly increased levels of EBV DNA associated with NK-cell lymphocytosis, and complications including chronic active EBV infection, hypersensitivity to mosquito bites, and hemophagocytic syndrome. Five patients with severe disease died of EBV-associated NK/T-cell lymphomas or hemophagocytic syndrome 2 to 14 years after onset. Conclusion: Both typical and severe HV are included within the spectrum of cutaneous disorders mediated by EBV-infected T cells, and the severe HV group may have overt EBV-associated NK/T-cell lymphoproliferative disorders with a frequently fatal outcome.",
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T1 - Pathogenic link between hydroa vacciniforme and Epstein-Barr virus-associated hematologic disorders

AU - Iwatsuki, Keiji

AU - Satoh, Masataka

AU - Yamamoto, Takenobu

AU - Oono, Takashi

AU - Morizane, Shin

AU - Ohtsuka, Mikio

AU - Xu, Zi Gang

AU - Suzuki, Daisuke

AU - Tsuji, Kazuhide

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AB - Objectives: To determine the pathogenic association of latent Epstein-Barr virus (EBV) infections with both typical hydroa vacciniforme (HV) and severe HV-like eruptions, and to survey the complications and outcomes of patients. Design: Case series. Patients: Twenty-nine patients with HV or severe HV-like eruptions. Interventions: In situ hybridization and immunostaining of biopsy specimens; extraction of DNA samples from cutaneous lesions and/or peripheral blood mononuclear cells for EBV DNA assay. Main Outcome Measures: Clinicopathologic manifestations, hematologic findings, complications, and outcomes; presence of latent EBV infection. Results: T cells positive for EBV-encoded small nuclear RNA (EBER) were detected, to various degrees, in cutaneous infiltrates in 28 (97%) of 29 patients, including all 6 patients with definite HV with a positive phototest reaction, 11 of 12 patients with probable HV without evidence of photosensitivity, and all 11 patients with severe HV associated with systemic symptoms. In addition to EBER-positive T cells, many cytotoxic T lymphocytes expressing T-cell intracellular antigen 1 and granzyme B were present in the cutaneous lesions. Natural killer (NK) cells were absent or at a background level. The UV-induced cutaneous lesions showed histopathologic findings consistent with those of HV, containing many EBER-positive cells. Although no hematologic abnormalities were found in the definite and probable HV groups, the amounts of EBV DNA were increased in the peripheral blood mononuclear cells. By contrast, the severe HV group had markedly increased levels of EBV DNA associated with NK-cell lymphocytosis, and complications including chronic active EBV infection, hypersensitivity to mosquito bites, and hemophagocytic syndrome. Five patients with severe disease died of EBV-associated NK/T-cell lymphomas or hemophagocytic syndrome 2 to 14 years after onset. Conclusion: Both typical and severe HV are included within the spectrum of cutaneous disorders mediated by EBV-infected T cells, and the severe HV group may have overt EBV-associated NK/T-cell lymphoproliferative disorders with a frequently fatal outcome.

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