Passive oral immunization by egg yolk immunoglobulin (IgY) to vibrio cholerae effectively prevents cholera

Kazuyuki Hirai, Hideyuki Arimitsu, Koji Umeda, Kenji Yokota, Lianhu Shen, Kiyoshi Ayada, Yoshikatsu Kodama, Takao Tsuji, Yoshikazu Hirai, Keiji Oguma

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

In an attempt to prepare egg yolk immunoglobulin (IgY) to treat and prevent cholera, hens were immunized by a mixture of heat- or formalin-killed Vibrio cholerae O1 and O139 organisms, or by the recombinant cholera toxin B subunit (CTB). The IgYs were partially purified from egg yolk and orally administered to suckling mice before or after challenge with live O1 or O139 cells. The anti-O1 and O139 IgYs and the mixture of either IgY with anti-CTB IgY significantly protected the occurrence of cholera caused by both O1 and O139 infection. Since large amounts of IgY can be prepared very easily and at low cost, this seems to be a useful procedure for preventing and treating cholera.

Original languageEnglish
Pages (from-to)163-170
Number of pages8
JournalActa Medica Okayama
Volume64
Issue number3
Publication statusPublished - Jul 2010

Fingerprint

Immunization
Egg Yolk
Passive Immunization
Vibrio cholerae
Cholera
Immunoglobulins
Cholera Toxin
Vibrio cholerae O139
Vibrio cholerae O1
Formaldehyde
Hot Temperature
Costs and Cost Analysis
IgY
Infection
Costs

Keywords

  • IgY
  • O1
  • O139
  • Vibrio cholerae

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Hirai, K., Arimitsu, H., Umeda, K., Yokota, K., Shen, L., Ayada, K., ... Oguma, K. (2010). Passive oral immunization by egg yolk immunoglobulin (IgY) to vibrio cholerae effectively prevents cholera. Acta Medica Okayama, 64(3), 163-170.

Passive oral immunization by egg yolk immunoglobulin (IgY) to vibrio cholerae effectively prevents cholera. / Hirai, Kazuyuki; Arimitsu, Hideyuki; Umeda, Koji; Yokota, Kenji; Shen, Lianhu; Ayada, Kiyoshi; Kodama, Yoshikatsu; Tsuji, Takao; Hirai, Yoshikazu; Oguma, Keiji.

In: Acta Medica Okayama, Vol. 64, No. 3, 07.2010, p. 163-170.

Research output: Contribution to journalArticle

Hirai, K, Arimitsu, H, Umeda, K, Yokota, K, Shen, L, Ayada, K, Kodama, Y, Tsuji, T, Hirai, Y & Oguma, K 2010, 'Passive oral immunization by egg yolk immunoglobulin (IgY) to vibrio cholerae effectively prevents cholera', Acta Medica Okayama, vol. 64, no. 3, pp. 163-170.
Hirai, Kazuyuki ; Arimitsu, Hideyuki ; Umeda, Koji ; Yokota, Kenji ; Shen, Lianhu ; Ayada, Kiyoshi ; Kodama, Yoshikatsu ; Tsuji, Takao ; Hirai, Yoshikazu ; Oguma, Keiji. / Passive oral immunization by egg yolk immunoglobulin (IgY) to vibrio cholerae effectively prevents cholera. In: Acta Medica Okayama. 2010 ; Vol. 64, No. 3. pp. 163-170.
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