Parvalbumin, calretinin and carbonic anhydrase in the trigeminal and spinal primary neurons of the rat

Hiroyuki Ichikawa, Toru Deguchi, Tadao Nakago, David M. Jacobowitz, Tomosada Sugimoto

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76 Citations (Scopus)

Abstract

The cell-body size of parvalbumin-immunoreactive (-ir) primary neurons was measured in the trigeminal (TG) and lumber dorsal root ganglia (DRG). In the DRG, parvalbumin-ir was mostly detected in large cells (94% in the range of 600-2800 μm2). Parvalbumin-ir TG cells were smaller than similar DRG cells and yet parvalbumin-ir TG cells of <400 μm2 (2.86%) were rare. Trichrome stains for parvalbumin, calretinin (CR) and carbonic anhydrase (CA), and for parvalbumin, calcitonin gene-related peptide (CGRP) and CA were performed to estimate possible overlap of these substances. Virtually all parvalbumin-ir DRG cells contained CA activity while a small subpopulation (28.5%) of CR-ir DRG cells lacked CA activity. All the CR-ir DRG cells that exhibited CA were also ir for parvalbumin. 31.1% of parvalbumin-ir DRG cells exhibited CR-ir while 71.5% of CR-ir DRG cells showed parvalbumin-ir. All the CR-ir DRG cells of <400 μm2 lacked CA activity and parvalbumin-ir while all those of > 800 μm2 exhibited both activities. ∼30% of CR-ir DRG cells in the size range of 400-800 μm2 co-expressed CA. DRG cel co-expressing parvalbumin and CGRP were rare (∼1%). As was the case for the DRG, most of parvalbumin-ir TG cells exhibited CA activity (89.24%) and lacked CGRP-ir (96.6%). CR-ir TG cells were also subdivided into two groups; one with and the other without co-expression of CA. Unlike in the DRG, however, co-expression of parvalbumin and CR could never be detected in the TG.

Original languageEnglish
Pages (from-to)241-245
Number of pages5
JournalBrain Research
Volume655
Issue number1-2
DOIs
Publication statusPublished - Aug 29 1994

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Keywords

  • Calretinin
  • Carbonic anhydrase
  • Dorsal root ganglion
  • Parvalbumin
  • Rat
  • Trigeminal ganglion

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

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