Parvalbumin- and calretinin-immunoreactive trigeminal neurons innervating the rat molar tooth pulp

Hiroyuki Ichikawa, Toru Deguchi, Tadao Nakago, David M. Jacobowitz, Tomosada Sugimoto

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Calcium-binding proteins and neuropeptides were examined in trigeminal neuronal cell bodies retrogradely labeled with Fast blue (FB) from the maxillary molar tooth pulp of the rat. FB-labeled cells were located in the maxillary division of the trigeminal ganglion. ∼ 30 and 50% of the labeled cells were immunoreactive for parvalbumin and calcitonin gene-related peptide (CGRP), respectively. The coexpression of these substances was observed in 9.5% of FB-labeled cells. On the other hand, 2.4% of FB-labeled cells exhibited calretinin-immunoreactivity (CR-ir) and 20% tachykinin (TK)-ir. The coexpression of CR and TK was observed in 1.9% of FB-labeled cells, i.e., most of CR-ir FB-labeled neurons coexpressed TK-ir. An immuno-EM method revealed that all parvalbumin-ir nerve fibers in the root pulp were myelinated and that CGRP-ir nerve fibers were both myelinated (15%) and unmyelinated (85%). The present study indicated that primary nociceptors innervating the rat molar both pulp contained parvalbumin and CR and coexpressed these calcium-binding proteins and neuropeptides. It was suggested that peripheral axons of parvalbumin-ir tooth pulp primary neurons are all myelinated. Most peripheral CR-ir axons are probably unmyelinated because TK-ir myelinated axons have never been demonstrated in any peripheral organ.

Original languageEnglish
Pages (from-to)205-211
Number of pages7
JournalBrain Research
Volume679
Issue number2
DOIs
Publication statusPublished - May 15 1995
Externally publishedYes

Fingerprint

Calbindin 2
Parvalbumins
Tachykinins
Tooth
Neurons
Axons
Calcium-Binding Proteins
Calcitonin Gene-Related Peptide
Neuropeptides
Nerve Fibers
Trigeminal Ganglion
Nociceptors
Deciduous Tooth
diamidino compound 253-50

Keywords

  • Calretinin
  • Parvalbumin
  • Rat
  • Tooth pulp
  • Trigeminal ganglion

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Parvalbumin- and calretinin-immunoreactive trigeminal neurons innervating the rat molar tooth pulp. / Ichikawa, Hiroyuki; Deguchi, Toru; Nakago, Tadao; Jacobowitz, David M.; Sugimoto, Tomosada.

In: Brain Research, Vol. 679, No. 2, 15.05.1995, p. 205-211.

Research output: Contribution to journalArticle

Ichikawa, H, Deguchi, T, Nakago, T, Jacobowitz, DM & Sugimoto, T 1995, 'Parvalbumin- and calretinin-immunoreactive trigeminal neurons innervating the rat molar tooth pulp', Brain Research, vol. 679, no. 2, pp. 205-211. https://doi.org/10.1016/0006-8993(95)00234-H
Ichikawa, Hiroyuki ; Deguchi, Toru ; Nakago, Tadao ; Jacobowitz, David M. ; Sugimoto, Tomosada. / Parvalbumin- and calretinin-immunoreactive trigeminal neurons innervating the rat molar tooth pulp. In: Brain Research. 1995 ; Vol. 679, No. 2. pp. 205-211.
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