Participation of the hup gene product in site-specific DNA inversion in Escherichia coli

Morimasa Wada, Kazuhiro Kutsukake, Teruya Komano, Fumio Imamoto, Yasunobu Kano

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42 Citations (Scopus)

Abstract

The closely related Escherichia coli genes hupA and hupB each encode a bacterial histone-like protein HU. We report here that DNA inversion mediated by hin, gin, pin and rci but not by cin is blocked in a hupA hupB double mutant, although inversions in these systems occur in the hupA or hupB single mutant as efficiently as in the wild-type strain. These findings show that HU protein participates in site-specific DNA inversion in E. coli and that only one subunit, either HU-1 or HU-2, is sufficient for this inversion.

Original languageEnglish
Pages (from-to)345-352
Number of pages8
JournalGene
Volume76
Issue number2
DOIs
Publication statusPublished - Mar 30 1989

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Keywords

  • HU protein
  • Histone-like protein
  • cin, gin, hin, pin systems
  • hupA hupB mutant
  • recombinant DNA
  • recombinase
  • shufflon

ASJC Scopus subject areas

  • Genetics

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