Participation of the hup gene product in site-specific DNA inversion in Escherichia coli

Morimasa Wada, Kazuhiro Kutsukake, Teruya Komano, Fumio Imamoto, Yasunobu Kano

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The closely related Escherichia coli genes hupA and hupB each encode a bacterial histone-like protein HU. We report here that DNA inversion mediated by hin, gin, pin and rci but not by cin is blocked in a hupA hupB double mutant, although inversions in these systems occur in the hupA or hupB single mutant as efficiently as in the wild-type strain. These findings show that HU protein participates in site-specific DNA inversion in E. coli and that only one subunit, either HU-1 or HU-2, is sufficient for this inversion.

Original languageEnglish
Pages (from-to)345-352
Number of pages8
JournalGene
Volume76
Issue number2
DOIs
Publication statusPublished - Mar 30 1989
Externally publishedYes

Fingerprint

Escherichia coli
DNA
Histones
Genes
Proteins

Keywords

  • cin, gin, hin, pin systems
  • Histone-like protein
  • HU protein
  • hupA hupB mutant
  • recombinant DNA
  • recombinase
  • shufflon

ASJC Scopus subject areas

  • Genetics

Cite this

Participation of the hup gene product in site-specific DNA inversion in Escherichia coli. / Wada, Morimasa; Kutsukake, Kazuhiro; Komano, Teruya; Imamoto, Fumio; Kano, Yasunobu.

In: Gene, Vol. 76, No. 2, 30.03.1989, p. 345-352.

Research output: Contribution to journalArticle

Wada, M, Kutsukake, K, Komano, T, Imamoto, F & Kano, Y 1989, 'Participation of the hup gene product in site-specific DNA inversion in Escherichia coli', Gene, vol. 76, no. 2, pp. 345-352. https://doi.org/10.1016/0378-1119(89)90174-1
Wada, Morimasa ; Kutsukake, Kazuhiro ; Komano, Teruya ; Imamoto, Fumio ; Kano, Yasunobu. / Participation of the hup gene product in site-specific DNA inversion in Escherichia coli. In: Gene. 1989 ; Vol. 76, No. 2. pp. 345-352.
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