Participation of the CYP2D subfamily in lidocaine 3-hydroxylation and formation of a reactive metabolite covalently bound to liver microsomal protein in rats

Yasuhiro Masubuchi, Shin Umeda, Shigeki Igarashi, Shoichi Fujita, Shizuo Narimatsu, Tokuji Suzuki

Research output: Contribution to journalArticle

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Abstract

Lidocaine metabolism was investigated in rat liver microsomes and in a reconstituted system containing P450BTL, a cytochrome (P450) isozyme belonging to the CYP2D subfamily (Suzuki et al., Drug Metab Dispos 20: 367-373, 1992). P450BTL biotransformed lidocaine into 3-hydroxylidocaine (3-OH-LID) but not monoethylglycinexylidide and 2-methylhydroxylidocaine, in the reconstituted system including NADPH-P450 reductase and dilauroylphosphatidylcholine. An antibody against P450BTL inhibited microsomal lidocaine 3-hydroxylase activity by 97%. Thus, P450BTL and/or its immunorelated P450 isozyme(s) belonging to the CYP2D subfamily appear to be involved in lidocaine 3-hydroxylation. Furthermore, the antibody also suppressed the amounts of a lidocaine metabolite(s) bound to microsomal protein. These results suggest that the CYP2D subfamily biotransformed lidocaine into 3-OH-LID via an epoxy intermediate, which binds to microsomal macromolecules.

Original languageEnglish
Pages (from-to)1867-1869
Number of pages3
JournalBiochemical Pharmacology
Volume46
Issue number10
DOIs
Publication statusPublished - Nov 17 1993
Externally publishedYes

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Hydroxylation
Metabolites
Lidocaine
Liver
Rats
Proteins
monoethylglycinexylidide
Isoenzymes
NADPH-Ferrihemoprotein Reductase
Antibodies
Liver Microsomes
Mixed Function Oxygenases
Macromolecules
Metabolism
Cytochrome P-450 Enzyme System
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Pharmacology

Cite this

Participation of the CYP2D subfamily in lidocaine 3-hydroxylation and formation of a reactive metabolite covalently bound to liver microsomal protein in rats. / Masubuchi, Yasuhiro; Umeda, Shin; Igarashi, Shigeki; Fujita, Shoichi; Narimatsu, Shizuo; Suzuki, Tokuji.

In: Biochemical Pharmacology, Vol. 46, No. 10, 17.11.1993, p. 1867-1869.

Research output: Contribution to journalArticle

Masubuchi, Yasuhiro ; Umeda, Shin ; Igarashi, Shigeki ; Fujita, Shoichi ; Narimatsu, Shizuo ; Suzuki, Tokuji. / Participation of the CYP2D subfamily in lidocaine 3-hydroxylation and formation of a reactive metabolite covalently bound to liver microsomal protein in rats. In: Biochemical Pharmacology. 1993 ; Vol. 46, No. 10. pp. 1867-1869.
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