Participation of Rho-dependent transcription termination in oxidative stress sensitivity caused by an rpoB mutation

Nobuyuki Kawamura, Kenji Kurokawa, Takahiro Ito, Hiroshi Hamamoto, Hiroshi Koyama, Chikara Kaito, Kazuhisa Sekimizu

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The role of transcription termination process for gene expression regulation is poorly understood. Either a multicopy supply of the rof gene or bicyclomycin, both of which inhibit the transcription termination Rho factor, suppressed the increased sensitivity to oxidative stress of the rifampicin-resistant rpoB mutation in Escherichia coli. Multi-copy supply of the rnk gene also suppressed oxidative stress sensitivity, coincident with the recovery of the reduced concentration of nucleoside triphosphates in the mutant cells, which is one of the factors that affects transcription termination efficiency in vitro. Thus, an appropriate, nonexcessive termination frequency at Rho-dependent transcription terminators might contribute to oxidative stress survival. Clinical application of oxidative stress against drug resistant bacteria is also discussed.

Original languageEnglish
Pages (from-to)477-487
Number of pages11
JournalGenes to Cells
Volume10
Issue number5
DOIs
Publication statusPublished - May 1 2005
Externally publishedYes

Fingerprint

Oxidative Stress
Mutation
Rho Factor
Gene Expression Regulation
Rifampin
Nucleosides
Genes
Transcription Factors
Escherichia coli
Bacteria
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Participation of Rho-dependent transcription termination in oxidative stress sensitivity caused by an rpoB mutation. / Kawamura, Nobuyuki; Kurokawa, Kenji; Ito, Takahiro; Hamamoto, Hiroshi; Koyama, Hiroshi; Kaito, Chikara; Sekimizu, Kazuhisa.

In: Genes to Cells, Vol. 10, No. 5, 01.05.2005, p. 477-487.

Research output: Contribution to journalArticle

Kawamura, Nobuyuki ; Kurokawa, Kenji ; Ito, Takahiro ; Hamamoto, Hiroshi ; Koyama, Hiroshi ; Kaito, Chikara ; Sekimizu, Kazuhisa. / Participation of Rho-dependent transcription termination in oxidative stress sensitivity caused by an rpoB mutation. In: Genes to Cells. 2005 ; Vol. 10, No. 5. pp. 477-487.
@article{529c2d06b5964a2eae04a7ba003c10f4,
title = "Participation of Rho-dependent transcription termination in oxidative stress sensitivity caused by an rpoB mutation",
abstract = "The role of transcription termination process for gene expression regulation is poorly understood. Either a multicopy supply of the rof gene or bicyclomycin, both of which inhibit the transcription termination Rho factor, suppressed the increased sensitivity to oxidative stress of the rifampicin-resistant rpoB mutation in Escherichia coli. Multi-copy supply of the rnk gene also suppressed oxidative stress sensitivity, coincident with the recovery of the reduced concentration of nucleoside triphosphates in the mutant cells, which is one of the factors that affects transcription termination efficiency in vitro. Thus, an appropriate, nonexcessive termination frequency at Rho-dependent transcription terminators might contribute to oxidative stress survival. Clinical application of oxidative stress against drug resistant bacteria is also discussed.",
author = "Nobuyuki Kawamura and Kenji Kurokawa and Takahiro Ito and Hiroshi Hamamoto and Hiroshi Koyama and Chikara Kaito and Kazuhisa Sekimizu",
year = "2005",
month = "5",
day = "1",
doi = "10.1111/j.1365-2443.2005.00849.x",
language = "English",
volume = "10",
pages = "477--487",
journal = "Genes to Cells",
issn = "1356-9597",
publisher = "Wiley-Blackwell",
number = "5",

}

TY - JOUR

T1 - Participation of Rho-dependent transcription termination in oxidative stress sensitivity caused by an rpoB mutation

AU - Kawamura, Nobuyuki

AU - Kurokawa, Kenji

AU - Ito, Takahiro

AU - Hamamoto, Hiroshi

AU - Koyama, Hiroshi

AU - Kaito, Chikara

AU - Sekimizu, Kazuhisa

PY - 2005/5/1

Y1 - 2005/5/1

N2 - The role of transcription termination process for gene expression regulation is poorly understood. Either a multicopy supply of the rof gene or bicyclomycin, both of which inhibit the transcription termination Rho factor, suppressed the increased sensitivity to oxidative stress of the rifampicin-resistant rpoB mutation in Escherichia coli. Multi-copy supply of the rnk gene also suppressed oxidative stress sensitivity, coincident with the recovery of the reduced concentration of nucleoside triphosphates in the mutant cells, which is one of the factors that affects transcription termination efficiency in vitro. Thus, an appropriate, nonexcessive termination frequency at Rho-dependent transcription terminators might contribute to oxidative stress survival. Clinical application of oxidative stress against drug resistant bacteria is also discussed.

AB - The role of transcription termination process for gene expression regulation is poorly understood. Either a multicopy supply of the rof gene or bicyclomycin, both of which inhibit the transcription termination Rho factor, suppressed the increased sensitivity to oxidative stress of the rifampicin-resistant rpoB mutation in Escherichia coli. Multi-copy supply of the rnk gene also suppressed oxidative stress sensitivity, coincident with the recovery of the reduced concentration of nucleoside triphosphates in the mutant cells, which is one of the factors that affects transcription termination efficiency in vitro. Thus, an appropriate, nonexcessive termination frequency at Rho-dependent transcription terminators might contribute to oxidative stress survival. Clinical application of oxidative stress against drug resistant bacteria is also discussed.

UR - http://www.scopus.com/inward/record.url?scp=17844400790&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=17844400790&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2443.2005.00849.x

DO - 10.1111/j.1365-2443.2005.00849.x

M3 - Article

C2 - 15836776

AN - SCOPUS:17844400790

VL - 10

SP - 477

EP - 487

JO - Genes to Cells

JF - Genes to Cells

SN - 1356-9597

IS - 5

ER -