Participation of histaminergic H 1 and noradrenergic α 1 receptors in orexin A-induced wakefulness in rats

Yuki Shigemoto, Yoko Fujii, Kazuaki Shinomiya, Chiaki Kamei

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The participation of histaminergic H 1 and noradrenergic α 1 receptors in orexin A-induced wakefulness was studied by examining the sleep-wakefulness cycle in rats. Intracerebroventricular infusion of orexin A (1 nmol) caused an increase in the wakefulness state, while non-rapid eye movement sleep (NREM sleep) and rapid eye movement sleep (REM sleep) states were decreased. Prazosin (150 nmol) showed no significant antagonistic effect on the orexin A-induced increase in the wakefulness state and decrease in NREM and REM sleep. On the contrary, pyrilamine (150 nmol) was effective in antagonizing orexin A-induced increase in wakefulness and decrease in NREM sleep. When prazosin (150 nmol) and pyrilamine (150 nmol) were simultaneously perfused into the lateral ventricle, an almost complete antagonistic effect was observed with the increase in the wakefulness state and decrease in NREM sleep. Orexin A (1 nmol) caused a significant decrease in the histamine contents of the cortex, hippocampus and hypothalamus, whereas noradrenaline contents were decreased only in the hypothalamus. From these results, we concluded that the arousal effect induced by orexin A occurs through histaminergic H 1 and noradrenergic α 1 receptors, although participation of the H 1 receptor was more important than the α 1 receptor.

Original languageEnglish
Pages (from-to)121-125
Number of pages5
JournalBrain Research
Volume1023
Issue number1
DOIs
Publication statusPublished - Oct 8 2004

Keywords

  • Histamine
  • Noradrenaline
  • Orexin A
  • Rat
  • Sleep-wakefulness cycle

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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