Participation of cathepsin L on bone resorption

Hisao Kakegawa, Takeshi Nikawa, Kahori Tagami, Hiroshi Kamioka, Koji Sumitani, Terushige Kawata, Marinka Drobnič-Kosorok, Brigita Lenarčič, Vito Turk, Nobuhiko Katunuma

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

The proteinase responsible for bone collagen degradation in osteo-resorption was examined. The bone pit formation induced by parathyroid hormone (PTH) was markedly suppressed by leupeptin, E-64 and cystatin A, while no inhibition was observed by CA-074, a specific inhibitor of cathepsin B. Pig leucocyte cysteine proteinase inhibitor (PLCPI), a specific inhibitor of cathepsin L, and chymostatin, a selective inhibitor of cathepsin L, completely inhibited the pit formation. Cathepsin L activity in osteoclasts was much higher than the other cathepsin activities. Serum calcium in rats placed on a low calcium diet was decreased by treatment of E-64 or cystatin A, but not by CA-074. These findings suggest that cathepsin L is the main proteinase responsible for bone collagen degradation.

Original languageEnglish
Pages (from-to)247-250
Number of pages4
JournalFEBS Letters
Volume321
Issue number2-3
DOIs
Publication statusPublished - Apr 26 1993
Externally publishedYes

Fingerprint

Cathepsin L
Bone Resorption
Bone
Cystatin A
Peptide Hydrolases
Collagen
Calcium
Bone and Bones
Cathepsins
Degradation
Cathepsin B
Osteoclasts
Nutrition
Parathyroid Hormone
Osteogenesis
Rats
Diet
Serum
E 64
N-(3-propylcarbamoyloxirane-2-carbonyl)-isoleucyl-proline

Keywords

  • Bone resorption
  • CA-074
  • Cathepsin L
  • Cystatin A
  • E-64
  • Pig leucocyte cysteine proteinase inhibitor

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Kakegawa, H., Nikawa, T., Tagami, K., Kamioka, H., Sumitani, K., Kawata, T., ... Katunuma, N. (1993). Participation of cathepsin L on bone resorption. FEBS Letters, 321(2-3), 247-250. https://doi.org/10.1016/0014-5793(93)80118-E

Participation of cathepsin L on bone resorption. / Kakegawa, Hisao; Nikawa, Takeshi; Tagami, Kahori; Kamioka, Hiroshi; Sumitani, Koji; Kawata, Terushige; Drobnič-Kosorok, Marinka; Lenarčič, Brigita; Turk, Vito; Katunuma, Nobuhiko.

In: FEBS Letters, Vol. 321, No. 2-3, 26.04.1993, p. 247-250.

Research output: Contribution to journalArticle

Kakegawa, H, Nikawa, T, Tagami, K, Kamioka, H, Sumitani, K, Kawata, T, Drobnič-Kosorok, M, Lenarčič, B, Turk, V & Katunuma, N 1993, 'Participation of cathepsin L on bone resorption', FEBS Letters, vol. 321, no. 2-3, pp. 247-250. https://doi.org/10.1016/0014-5793(93)80118-E
Kakegawa H, Nikawa T, Tagami K, Kamioka H, Sumitani K, Kawata T et al. Participation of cathepsin L on bone resorption. FEBS Letters. 1993 Apr 26;321(2-3):247-250. https://doi.org/10.1016/0014-5793(93)80118-E
Kakegawa, Hisao ; Nikawa, Takeshi ; Tagami, Kahori ; Kamioka, Hiroshi ; Sumitani, Koji ; Kawata, Terushige ; Drobnič-Kosorok, Marinka ; Lenarčič, Brigita ; Turk, Vito ; Katunuma, Nobuhiko. / Participation of cathepsin L on bone resorption. In: FEBS Letters. 1993 ; Vol. 321, No. 2-3. pp. 247-250.
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