Participation of cathepsin L on bone resorption

Hisao Kakegawa, Takeshi Nikawa, Kahori Tagami, Hiroshi Kamioka, Koji Sumitani, Terushige Kawata, Marinka Drobnič-Kosorok, Brigita Lenarčič, Vito Turk, Nobuhiko Katunuma

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

The proteinase responsible for bone collagen degradation in osteo-resorption was examined. The bone pit formation induced by parathyroid hormone (PTH) was markedly suppressed by leupeptin, E-64 and cystatin A, while no inhibition was observed by CA-074, a specific inhibitor of cathepsin B. Pig leucocyte cysteine proteinase inhibitor (PLCPI), a specific inhibitor of cathepsin L, and chymostatin, a selective inhibitor of cathepsin L, completely inhibited the pit formation. Cathepsin L activity in osteoclasts was much higher than the other cathepsin activities. Serum calcium in rats placed on a low calcium diet was decreased by treatment of E-64 or cystatin A, but not by CA-074. These findings suggest that cathepsin L is the main proteinase responsible for bone collagen degradation.

Original languageEnglish
Pages (from-to)247-250
Number of pages4
JournalFEBS Letters
Volume321
Issue number2-3
DOIs
Publication statusPublished - Apr 26 1993
Externally publishedYes

Keywords

  • Bone resorption
  • CA-074
  • Cathepsin L
  • Cystatin A
  • E-64
  • Pig leucocyte cysteine proteinase inhibitor

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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  • Cite this

    Kakegawa, H., Nikawa, T., Tagami, K., Kamioka, H., Sumitani, K., Kawata, T., Drobnič-Kosorok, M., Lenarčič, B., Turk, V., & Katunuma, N. (1993). Participation of cathepsin L on bone resorption. FEBS Letters, 321(2-3), 247-250. https://doi.org/10.1016/0014-5793(93)80118-E