Participation of a dominant cytotoxic T cell population defined by a monoclonal antibody in syngeneic anti-tumor responses

Yuji Matsubayashi, Toshiyasu Hirama, Atsuo Morioka, Michihiro Iwashiro, Tohru Masuda, Haruto Uchino, Sunao Takeshita, Hideo Yamagishi, Heiichiro Udono, Masahiro Mieno, Eiichi Nakayama, Hiroshi Shiku, Akiko Uenaka, Kagemasa Kuribayashi

Research output: Contribution to journalArticle

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Abstract

Cytotoxic T lymphocyte (CTL) clones against a syngeneic Friend virus-induced erythroleukemia (FBL-3) were generated in C57BL/6 (B6) mice. A monoclonal antibody (mAb, N9-127) was then raised from spleen cells of a B6 mouse immunized syngenically against one of these CTL clones. This mAb detected the epitope (127Ep) of the T cell antigen receptor (TcR) on the immunizing CTL clone in tests of immunoprecipitation, specific blocking and proliferation, and induction of TcR-mediated nonspecific lysis of the clone. In addition, more than 10% of the FBL-3-specific CTL clones isolated independently from B6 mice were 127Ep+. Further investigations revealed that up to 30% of B6 anti-FBL-3 T cell blasts from mixed lymphocyte tumor cell cultures were positive for this epitope, and that its expression was confined to CD8+ T cells. This epitope was not detected in naive lymphoid cells from the spleen, lymph nodes or thymus or in T cell clones specific for tumors other than FBL-3. The FBL-3-specific CTL clones were next grouped into 127Ep+ and 127Ep- clones. Sequence analyses of the CTL clone used for immunization showed the rearrangements of Vα1Jα112-2 and Vβ10Dβ2.1Jβ2.7. Southern blot analysis of all the 127Ep+ CTL clones examined showed the same DNA rearrangement bands of both the TcR α and β genes. These findings suggested that mAb N9-127 recognized the shared determinant of the TcR molecule which was expressed by the dominant CTL population in the response to FBL-3.

Original languageEnglish
Pages (from-to)2095-2103
Number of pages9
JournalEuropean Journal of Immunology
Volume20
Issue number9
Publication statusPublished - Sep 1990
Externally publishedYes

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Cytotoxic T-Lymphocytes
Clone Cells
Monoclonal Antibodies
T-Lymphocytes
Population
Neoplasms
T-Cell Antigen Receptor
Epitopes
Spleen
Lymphocytes
Friend murine leukemia virus
T-Cell Receptor Genes
Leukemia, Erythroblastic, Acute
Gene Rearrangement
Southern Blotting
Immunoprecipitation
Thymus Gland
Sequence Analysis
Immunization
Cell Culture Techniques

ASJC Scopus subject areas

  • Immunology

Cite this

Matsubayashi, Y., Hirama, T., Morioka, A., Iwashiro, M., Masuda, T., Uchino, H., ... Kuribayashi, K. (1990). Participation of a dominant cytotoxic T cell population defined by a monoclonal antibody in syngeneic anti-tumor responses. European Journal of Immunology, 20(9), 2095-2103.

Participation of a dominant cytotoxic T cell population defined by a monoclonal antibody in syngeneic anti-tumor responses. / Matsubayashi, Yuji; Hirama, Toshiyasu; Morioka, Atsuo; Iwashiro, Michihiro; Masuda, Tohru; Uchino, Haruto; Takeshita, Sunao; Yamagishi, Hideo; Udono, Heiichiro; Mieno, Masahiro; Nakayama, Eiichi; Shiku, Hiroshi; Uenaka, Akiko; Kuribayashi, Kagemasa.

In: European Journal of Immunology, Vol. 20, No. 9, 09.1990, p. 2095-2103.

Research output: Contribution to journalArticle

Matsubayashi, Y, Hirama, T, Morioka, A, Iwashiro, M, Masuda, T, Uchino, H, Takeshita, S, Yamagishi, H, Udono, H, Mieno, M, Nakayama, E, Shiku, H, Uenaka, A & Kuribayashi, K 1990, 'Participation of a dominant cytotoxic T cell population defined by a monoclonal antibody in syngeneic anti-tumor responses', European Journal of Immunology, vol. 20, no. 9, pp. 2095-2103.
Matsubayashi Y, Hirama T, Morioka A, Iwashiro M, Masuda T, Uchino H et al. Participation of a dominant cytotoxic T cell population defined by a monoclonal antibody in syngeneic anti-tumor responses. European Journal of Immunology. 1990 Sep;20(9):2095-2103.
Matsubayashi, Yuji ; Hirama, Toshiyasu ; Morioka, Atsuo ; Iwashiro, Michihiro ; Masuda, Tohru ; Uchino, Haruto ; Takeshita, Sunao ; Yamagishi, Hideo ; Udono, Heiichiro ; Mieno, Masahiro ; Nakayama, Eiichi ; Shiku, Hiroshi ; Uenaka, Akiko ; Kuribayashi, Kagemasa. / Participation of a dominant cytotoxic T cell population defined by a monoclonal antibody in syngeneic anti-tumor responses. In: European Journal of Immunology. 1990 ; Vol. 20, No. 9. pp. 2095-2103.
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abstract = "Cytotoxic T lymphocyte (CTL) clones against a syngeneic Friend virus-induced erythroleukemia (FBL-3) were generated in C57BL/6 (B6) mice. A monoclonal antibody (mAb, N9-127) was then raised from spleen cells of a B6 mouse immunized syngenically against one of these CTL clones. This mAb detected the epitope (127Ep) of the T cell antigen receptor (TcR) on the immunizing CTL clone in tests of immunoprecipitation, specific blocking and proliferation, and induction of TcR-mediated nonspecific lysis of the clone. In addition, more than 10{\%} of the FBL-3-specific CTL clones isolated independently from B6 mice were 127Ep+. Further investigations revealed that up to 30{\%} of B6 anti-FBL-3 T cell blasts from mixed lymphocyte tumor cell cultures were positive for this epitope, and that its expression was confined to CD8+ T cells. This epitope was not detected in naive lymphoid cells from the spleen, lymph nodes or thymus or in T cell clones specific for tumors other than FBL-3. The FBL-3-specific CTL clones were next grouped into 127Ep+ and 127Ep- clones. Sequence analyses of the CTL clone used for immunization showed the rearrangements of Vα1Jα112-2 and Vβ10Dβ2.1Jβ2.7. Southern blot analysis of all the 127Ep+ CTL clones examined showed the same DNA rearrangement bands of both the TcR α and β genes. These findings suggested that mAb N9-127 recognized the shared determinant of the TcR molecule which was expressed by the dominant CTL population in the response to FBL-3.",
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AU - Iwashiro, Michihiro

AU - Masuda, Tohru

AU - Uchino, Haruto

AU - Takeshita, Sunao

AU - Yamagishi, Hideo

AU - Udono, Heiichiro

AU - Mieno, Masahiro

AU - Nakayama, Eiichi

AU - Shiku, Hiroshi

AU - Uenaka, Akiko

AU - Kuribayashi, Kagemasa

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N2 - Cytotoxic T lymphocyte (CTL) clones against a syngeneic Friend virus-induced erythroleukemia (FBL-3) were generated in C57BL/6 (B6) mice. A monoclonal antibody (mAb, N9-127) was then raised from spleen cells of a B6 mouse immunized syngenically against one of these CTL clones. This mAb detected the epitope (127Ep) of the T cell antigen receptor (TcR) on the immunizing CTL clone in tests of immunoprecipitation, specific blocking and proliferation, and induction of TcR-mediated nonspecific lysis of the clone. In addition, more than 10% of the FBL-3-specific CTL clones isolated independently from B6 mice were 127Ep+. Further investigations revealed that up to 30% of B6 anti-FBL-3 T cell blasts from mixed lymphocyte tumor cell cultures were positive for this epitope, and that its expression was confined to CD8+ T cells. This epitope was not detected in naive lymphoid cells from the spleen, lymph nodes or thymus or in T cell clones specific for tumors other than FBL-3. The FBL-3-specific CTL clones were next grouped into 127Ep+ and 127Ep- clones. Sequence analyses of the CTL clone used for immunization showed the rearrangements of Vα1Jα112-2 and Vβ10Dβ2.1Jβ2.7. Southern blot analysis of all the 127Ep+ CTL clones examined showed the same DNA rearrangement bands of both the TcR α and β genes. These findings suggested that mAb N9-127 recognized the shared determinant of the TcR molecule which was expressed by the dominant CTL population in the response to FBL-3.

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