Abstract
Cytotoxic T lymphocyte (CTL) clones against a syngeneic Friend virus-induced erythroleukemia (FBL-3) were generated in C57BL/6 (B6) mice. A monoclonal antibody (mAb, N9-127) was then raised from spleen cells of a B6 mouse immunized syngenically against one of these CTL clones. This mAb detected the epitope (127Ep) of the T cell antigen receptor (TcR) on the immunizing CTL clone in tests of immunoprecipitation, specific blocking and proliferation, and induction of TcR-mediated nonspecific lysis of the clone. In addition, more than 10% of the FBL-3-specific CTL clones isolated independently from B6 mice were 127Ep+. Further investigations revealed that up to 30% of B6 anti-FBL-3 T cell blasts from mixed lymphocyte tumor cell cultures were positive for this epitope, and that its expression was confined to CD8+ T cells. This epitope was not detected in naive lymphoid cells from the spleen, lymph nodes or thymus or in T cell clones specific for tumors other than FBL-3. The FBL-3-specific CTL clones were next grouped into 127Ep+ and 127Ep- clones. Sequence analyses of the CTL clone used for immunization showed the rearrangements of Vα1Jα112-2 and Vβ10Dβ2.1Jβ2.7. Southern blot analysis of all the 127Ep+ CTL clones examined showed the same DNA rearrangement bands of both the TcR α and β genes. These findings suggested that mAb N9-127 recognized the shared determinant of the TcR molecule which was expressed by the dominant CTL population in the response to FBL-3.
| Original language | English |
|---|---|
| Pages (from-to) | 2095-2103 |
| Number of pages | 9 |
| Journal | European Journal of Immunology |
| Volume | 20 |
| Issue number | 9 |
| Publication status | Published - Sep 1990 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
Cite this
Participation of a dominant cytotoxic T cell population defined by a monoclonal antibody in syngeneic anti-tumor responses. / Matsubayashi, Yuji; Hirama, Toshiyasu; Morioka, Atsuo; Iwashiro, Michihiro; Masuda, Tohru; Uchino, Haruto; Takeshita, Sunao; Yamagishi, Hideo; Udono, Heiichiro; Mieno, Masahiro; Nakayama, Eiichi; Shiku, Hiroshi; Uenaka, Akiko; Kuribayashi, Kagemasa.
In: European Journal of Immunology, Vol. 20, No. 9, 09.1990, p. 2095-2103.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Participation of a dominant cytotoxic T cell population defined by a monoclonal antibody in syngeneic anti-tumor responses
AU - Matsubayashi, Yuji
AU - Hirama, Toshiyasu
AU - Morioka, Atsuo
AU - Iwashiro, Michihiro
AU - Masuda, Tohru
AU - Uchino, Haruto
AU - Takeshita, Sunao
AU - Yamagishi, Hideo
AU - Udono, Heiichiro
AU - Mieno, Masahiro
AU - Nakayama, Eiichi
AU - Shiku, Hiroshi
AU - Uenaka, Akiko
AU - Kuribayashi, Kagemasa
PY - 1990/9
Y1 - 1990/9
N2 - Cytotoxic T lymphocyte (CTL) clones against a syngeneic Friend virus-induced erythroleukemia (FBL-3) were generated in C57BL/6 (B6) mice. A monoclonal antibody (mAb, N9-127) was then raised from spleen cells of a B6 mouse immunized syngenically against one of these CTL clones. This mAb detected the epitope (127Ep) of the T cell antigen receptor (TcR) on the immunizing CTL clone in tests of immunoprecipitation, specific blocking and proliferation, and induction of TcR-mediated nonspecific lysis of the clone. In addition, more than 10% of the FBL-3-specific CTL clones isolated independently from B6 mice were 127Ep+. Further investigations revealed that up to 30% of B6 anti-FBL-3 T cell blasts from mixed lymphocyte tumor cell cultures were positive for this epitope, and that its expression was confined to CD8+ T cells. This epitope was not detected in naive lymphoid cells from the spleen, lymph nodes or thymus or in T cell clones specific for tumors other than FBL-3. The FBL-3-specific CTL clones were next grouped into 127Ep+ and 127Ep- clones. Sequence analyses of the CTL clone used for immunization showed the rearrangements of Vα1Jα112-2 and Vβ10Dβ2.1Jβ2.7. Southern blot analysis of all the 127Ep+ CTL clones examined showed the same DNA rearrangement bands of both the TcR α and β genes. These findings suggested that mAb N9-127 recognized the shared determinant of the TcR molecule which was expressed by the dominant CTL population in the response to FBL-3.
AB - Cytotoxic T lymphocyte (CTL) clones against a syngeneic Friend virus-induced erythroleukemia (FBL-3) were generated in C57BL/6 (B6) mice. A monoclonal antibody (mAb, N9-127) was then raised from spleen cells of a B6 mouse immunized syngenically against one of these CTL clones. This mAb detected the epitope (127Ep) of the T cell antigen receptor (TcR) on the immunizing CTL clone in tests of immunoprecipitation, specific blocking and proliferation, and induction of TcR-mediated nonspecific lysis of the clone. In addition, more than 10% of the FBL-3-specific CTL clones isolated independently from B6 mice were 127Ep+. Further investigations revealed that up to 30% of B6 anti-FBL-3 T cell blasts from mixed lymphocyte tumor cell cultures were positive for this epitope, and that its expression was confined to CD8+ T cells. This epitope was not detected in naive lymphoid cells from the spleen, lymph nodes or thymus or in T cell clones specific for tumors other than FBL-3. The FBL-3-specific CTL clones were next grouped into 127Ep+ and 127Ep- clones. Sequence analyses of the CTL clone used for immunization showed the rearrangements of Vα1Jα112-2 and Vβ10Dβ2.1Jβ2.7. Southern blot analysis of all the 127Ep+ CTL clones examined showed the same DNA rearrangement bands of both the TcR α and β genes. These findings suggested that mAb N9-127 recognized the shared determinant of the TcR molecule which was expressed by the dominant CTL population in the response to FBL-3.
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M3 - Article
VL - 20
SP - 2095
EP - 2103
JO - European Journal of Immunology
JF - European Journal of Immunology
SN - 0014-2980
IS - 9
ER -