Participation of αIIbβ3 in platelet microparticle generation by collagen plus thrombin

Shosaku Nomura, Yutaka Komiyama, Eiji Matsuura, Gui Lan Xie, Kaoruko Katsura, Tetsuya Miyake, Yasuhiko Miyazaki, Hideo Kagawa, Takao Koike, Shirou Fukuhara

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16 Citations (Scopus)

Abstract

We investigated the role of αIIbβ3 in microparticle generation by normal and thrombasthenic platelets stimulated with collagen plus thrombin. Microparticle generation by normal platelets was scarcely inhibited by monoclonal antibodies for glyco-protein lb and glycoprotein IX. Although one monoclonal anti-α αIIbβ3 antibody (NNKY1-32) partly inhibited microparticle generation, 3 other monoclonal anti-α αIIbβ3 antibodies had little effect. However, the combination of 4 monoclonal anti- αIIbβ3 antibodies or treatment with a polyclonal anti- αIIbβ3 antibody significantly inhibited microparticle generation (p < 0.05). Microparticle generation by thrombasthenic platelets also occurred after stimulation with collagen plus thrombin, although at a significantly lower level compared with normal platelets. Monoclonal antibodies for resting αIIbβ3, P-selectin, activated αIIbβ3 and β2-glycoprotein I bound to microparticles from healthy platelets. In contrast, only a monoclonal antibody for β2-glycoprotein I bound to thrombasthenic microparticles. These results suggest that microparticle generation by collagen plus thrombin occurs via two different mechanisms which are dependent and independent of αIIbβ3, respectively. The αIIbβ3-dependent mechanism appears to require activation of αIIbβ3.

Original languageEnglish
Pages (from-to)31-37
Number of pages7
JournalPathophysiology of Haemostasis and Thrombosis
Volume26
Issue number1
DOIs
Publication statusPublished - Jan 1 1996

Keywords

  • Collagen plus thrombin
  • Flow cytometry
  • Glanzmann's thrombasthenia
  • Microparticle
  • αβ

ASJC Scopus subject areas

  • Hematology
  • Physiology (medical)

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    Nomura, S., Komiyama, Y., Matsuura, E., Xie, G. L., Katsura, K., Miyake, T., Miyazaki, Y., Kagawa, H., Koike, T., & Fukuhara, S. (1996). Participation of αIIbβ3 in platelet microparticle generation by collagen plus thrombin. Pathophysiology of Haemostasis and Thrombosis, 26(1), 31-37. https://doi.org/10.1159/000217185