Partial purification and characterization of dendritic cell differentiation factor.

K. Miyatani, K. Takahashi, Hiroyuki Yanai, Tadashi Yoshino, T. Akagi

Research output: Contribution to journalArticle

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Abstract

Previously, we reported that interleukin-2 (IL-2)-stimulated helper T cells produced an unknown soluble factor which induced dendritic cell-like differentiation in primary cultures of monocytic leukemia cells and we referred to this factor as dendritic cell differentiation factor (DCDF). In this study, we attempted to purify and characterize DCDF and investigated its biological effect on normal human monocytes. Gel filtration chromatography indicated that the molecular weight of DCDF is approximately 30-35 kDa. Chromatofocusing indicated that the isoelectric point of DCDF is approximately 5.0. DCDF, partially purified by subsequent gel filtration, chromatofocusing, and hydrophobic chromatography, significantly enhanced the HLA-DR expression of normal human monocytes and a human monocytic leukemia cell line, THP-1. This biological activity was not neutralized by any known antibodies to human cytokines. DCDF significantly amplified the T-cell stimulatory activity of monocytes in the allogeneic mixed leukocyte reaction (MLR). Moreover, DCDF significantly enhanced IL-1 beta and IL-6 production by monocytes in a dose-dependent manner. These results suggest that DCDF is a novel human cytokine which stimulates the accessory cell function of monocytes.

Original languageEnglish
Pages (from-to)67-72
Number of pages6
JournalActa Medica Okayama
Volume48
Issue number2
Publication statusPublished - Apr 1994

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Dendritic Cells
Purification
Cell Differentiation
Monocytes
T-cells
Gel Chromatography
Hydrophobic chromatography
Leukemia
Gels
Cytokines
Mixed Lymphocyte Culture Test
Isoelectric Point
Accessories
HLA-DR Antigens
Helper-Inducer T-Lymphocytes
Chromatography
Bioactivity
Interleukin-1beta
Interleukin-2
Interleukin-6

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Partial purification and characterization of dendritic cell differentiation factor. / Miyatani, K.; Takahashi, K.; Yanai, Hiroyuki; Yoshino, Tadashi; Akagi, T.

In: Acta Medica Okayama, Vol. 48, No. 2, 04.1994, p. 67-72.

Research output: Contribution to journalArticle

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