Parameters for measurement of oxidative stress in diabetes mellitus: Applicability of enzyme-linked immunosorbent assay for clinical evaluation

Eisei Noiri, Hirokazu Tsukahara

Research output: Contribution to journalArticle

17 Citations (Scopus)


Investigations of the mechanisms involved in the onset and progression of diabetes have recently confronted the role of reactive oxygen species (ROS) and oxidative stress. Prolonged exposure to hyperglycemic conditions induces nonenzymatic glycation of protein via the so-called Maillard reaction, resulting in Schiff-base products and Amadori products that engender ROS production. These processes initiate and exacerbate micro- and macrovascular complications in diabetes. Increased oxidative stress is induced by excessive ROS production and inadequate antioxidant defenses. Recently, oxidative stress status markers have been associated directly with the severity and prognosis of diabetes. To examine oxidative stress, reliable and high-throughput methods are needed to examine large numbers of clinical samples. The emerging availability of enzyme-linked immunosorbent assay (ELISA) for oxidative stress status markers allows its application to assessment of various pathophysiologic conditions, including diabetes. This review outlines the recent achievements of ELISA application for clinical studies elucidating oxidative stress. It introduces the potential applicability of ELISA for investigating oxidative stress in diabetes.

Original languageEnglish
Pages (from-to)167-175
Number of pages9
JournalJournal of Investigative Medicine
Issue number4
Publication statusPublished - May 2005
Externally publishedYes



  • Advanced glycation end products
  • Diabetes mellitus
  • Enzyme-linked immunosorbent assay
  • Fatty acid binding protein
  • Lipid peroxide
  • Nitric oxide
  • Oxidative stress
  • Peroxynitrite
  • Thioredoxin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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