Pancreas preservation with amphotericin B deteriorates islet yield for porcine islet isolation

Sayaka Sawada, Chika Miyagi-Shiohira, Kazuho Kuwae, Yoshihito Tamaki, Kai Nishime, Mayuko Sakai-Yonaha, Tasuku Yonaha, Issei Saitoh, Masami Watanabe, Hirofumi Noguchi

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Amphotericin B is a crucial agent in the management of serious systemic fungal infections. It is also known to be cytotoxic. In this study, we evaluated the effect of amphotericin B added to the preservation solution on islet yield during islet isolation. Methods: Porcine pancreata were preserved in the preservation solution with or without amphotericin B (0.25 μg/mL) for approximately 18 hours at 4°C, and then islet isolation was performed. An optimized number (1750 IE) of isolated islets from each group were transplanted into streptozotocin-induced diabetic mice. The culture of isolated islets and acinar tissue with amphotericin B was also evaluated. Results: The islet yield before and after purification in the amphotericin B (−) group was significantly higher than that in the amphotericin B (+) group. After islet transplantation into diabetic mice, blood glucose levels reached the normoglycemic range, with 50% and 0% of that of the diabetic mice in the amphotericin B (−) and amphotericin B (+) groups, respectively. In the culture study, amphotericin B was found to be cytotoxic to porcine islets and acinar tissue. Conclusions: Amphotericin B added to the preservation solution deteriorates islet yield during porcine islet isolation. Thus, the use of amphotericin B should be considered carefully for the preservation of the pancreas for islet isolation and islet culture before islet transplantation.

Original languageEnglish
JournalXenotransplantation
DOIs
Publication statusAccepted/In press - 2021
Externally publishedYes

Keywords

  • amphotericin B
  • islet transplantation
  • pancreas preservation
  • porcine pancreas

ASJC Scopus subject areas

  • Immunology
  • Transplantation

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