OY-TES-1 expression and serum immunoreactivity in epithelial ovarian cancer

Jonathan Tammela, Akiko Uenaka, Toshiro Ono, Yuji Noguchi, Achim A. Jungbluth, Paulette Mhawech-Fauceglia, Feng Qian, Sally Schneider, Sameer Sharma, Deborah Driscoll, Shashikant Lele, Lloyd J. Old, Eiichi Nakayama, Kunle Odunsi

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37 Citations (Scopus)

Abstract

OY-TES-1 is a novel target that belongs to the family of 'cancer/testis' (CT) antigens. Our goal was to examine the expression and immunogenicity of OY-TES-1 in epithelial ovarian cancer (EOC) to determine its potential as a target for vaccine therapy. OY-TES-1 expression was determined by one-step reverse transcriptase PCR on 100 EOC samples, 5 EOC cell lines, and a panel of normal tissues. Immunohistochemistry (IHC) was performed on the same panel of EOC tissues. Sera from a sub-group of patients were tested for OY-TES-1 antibody by ELISA. Thymus and leukocytes were weakly positive for OY-TES-1 while the remaining 5 normal tissues were negative. Expression of OY-TES-1 by either RT-PCR and/or IHC was demonstrable in 69/100 (69%) tumors. Humoral immunity to OY-TES-1 was demonstrated in 1/10 (10%) serum samples from patients whose tumors expressed the antigen. The median follow-up of the patient population was 34 months. There was no correlation between antigen expression and stage, grade, histology and survival. OY-TES-1 is expressed in 69% of patients with EOC, is absent from normal ovarian tissue, and a proportion of patients show evidence of a specific humoral immune response. These findings make OY-TES-1 an attractive target for antigen-specific immunotherapy in EOC.

Original languageEnglish
Pages (from-to)903-910
Number of pages8
JournalInternational journal of oncology
Volume29
Issue number4
Publication statusPublished - Oct 2006

Keywords

  • Antibody
  • Immunogenicity
  • Immunohistochemistry
  • Ovarian tumor antigen
  • Prognostic indicator

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Tammela, J., Uenaka, A., Ono, T., Noguchi, Y., Jungbluth, A. A., Mhawech-Fauceglia, P., Qian, F., Schneider, S., Sharma, S., Driscoll, D., Lele, S., Old, L. J., Nakayama, E., & Odunsi, K. (2006). OY-TES-1 expression and serum immunoreactivity in epithelial ovarian cancer. International journal of oncology, 29(4), 903-910.