Oxytocin modulates the pulsatile secretion of prostaglandin F(2α) in initiated luteolysis in cattle

J. Kotwica, D. Skarzynski, G. Miszkiel, P. Melin, K. Okuda

Research output: Contribution to journalArticle

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Abstract

Subluteolytic doses of prostaglandin F(2α) analogue (oestrophan) given i.m. and oxytocin (OT) antagonist (CAP) and noradrenaline (NA) infused into the abdominal aorta were used to test the importance of luteal or in pulsatile secretion of prostaglandin F(2α) (PGF) during luteolysis in heifers (n = 17). In experiment 1, heifers were pre-infused for 30 minutes with saline on either day 17 of the oestrous cycle (group 1; n = 4) or on day 18 of the oestrous cycle (group 2; n = 3), and with CAP (8 mg per animal) on day 17 of the oestrous cycle (group 3; n = 4). Next, heifers were injected with oestrophan (30 μg per animal). Injection of oestrophan in Group 3 increased OT concentrations (P <0.001) to values similar to those observed during spontaneous luteolysis (50 to 70 pg ml-1). PGFM concentrations in this group also increased (P <0.001), but were lower (P <0.05) than the values in groups 1 and 2. CAP given prior to oestrophan decreased both PGFM elevation (P <0.06) and its area under the curve (P <0.01), compared to the saline pretreated heifers. In experiment 2 NA (4 mg) was infused twice for 30 minutes at five hour intervals to release or on day 17 of the oestrous cycle (n = 6). However, during hormone analysis it appeared that three of six heifers had elevated PGFM concentrations (group 1) and three others did not (group 2). NA caused the correlated increase of progesterone and or secretion (r = 0.68; P <0.05) in both groups but it only influenced PGF secretion in group 1 only (P <0.05). We postulate that or can amplify and modulate the course of induced luteolysis as a regulator of the amplitude of pulsatile PGF secretion. PGF analogue stimulates secretion of endogenous PGF from the uterus in cattle and this may be an important component of the luteolytic response to exogenous PGF.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalResearch in Veterinary Science
Volume66
Issue number1
DOIs
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

Luteolysis
luteolysis
oxytocin
Prostaglandins F
Oxytocin
prostaglandins
heifers
Cloprostenol
estrous cycle
secretion
norepinephrine
cattle
Norepinephrine
Synthetic Prostaglandins F
aorta
corpus luteum
uterus
antagonists
progesterone
animals

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Oxytocin modulates the pulsatile secretion of prostaglandin F(2α) in initiated luteolysis in cattle. / Kotwica, J.; Skarzynski, D.; Miszkiel, G.; Melin, P.; Okuda, K.

In: Research in Veterinary Science, Vol. 66, No. 1, 1999, p. 1-5.

Research output: Contribution to journalArticle

Kotwica, J. ; Skarzynski, D. ; Miszkiel, G. ; Melin, P. ; Okuda, K. / Oxytocin modulates the pulsatile secretion of prostaglandin F(2α) in initiated luteolysis in cattle. In: Research in Veterinary Science. 1999 ; Vol. 66, No. 1. pp. 1-5.
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