Oxygen concentration is an important factor for modulating progesterone synthesis in bovine corpus luteum

Ryo Nishimura, Ryosuke Sakumoto, Yuko Tatsukawa, Tomas J. Acosta, Kiyoshi Okuda

Research output: Contribution to journalArticle

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Abstract

Oxygen deficiency caused by a decrease in the blood supply is known to induce various responses of cells. Because luteal blood flow has been shown to decrease during luteolysis, a low-oxygen condition seems to be an integral part of the environment during luteolysis. To determine whether a low-oxygen condition is associated with functional luteolysis, we examined the influence of reduced oxygen tension on the luteal progesterone (P4) generating system in cultured bovine midluteal cells. Luteal cells obtained from midcycle corpus luteum (d 8-12) were incubated under different O2 concentrations (20, 10, 5, 3% O2) with or without LH for 24 h. P4 production decreased with decreasing O2 concentration but was significantly stimulated by LH regardless of O2 concentration. After 8 h of culture, both basal and LH-stimulated P4 production was significantly lower under 3% O2 than under 20% O2. Low-oxygen condition also inhibited pregnenolone production. Cytochrome P450 side-chain cleavage enzyme (P450scc) mRNA expression, measured by quantitative PCR, decreased under low-oxygen condition in both non-LH-treated and LH-treated cells. Low-oxygen condition did not affect the expressions of steroidogenic acute regulatory protein mRNA or protein, whereas steroidogenic acute regulatory protein mRNA expression was stimulated by LH during 4 h of culture. Low-oxygen condition also did not affect 3β-hydroxysteroid dehydrogenase/Δ5-Δ4 isomerase mRNA expression or the activity of the enzyme in the cells, regardless of the incubation period. The overall results indicate that a low-oxygen condition decreases P4 synthesis by attenuating P450scc production and P450scc activity in bovine luteal cells and suggest that oxygen deficiency is an essential condition for the progression of luteolysis in cattle.

Original languageEnglish
Pages (from-to)4273-4280
Number of pages8
JournalEndocrinology
Volume147
Issue number9
DOIs
Publication statusPublished - 2006

Fingerprint

Corpus Luteum
Progesterone
Oxygen
Luteolysis
Luteal Cells
Messenger RNA
Cytochrome P-450 Enzyme System
Enzymes
3-Hydroxysteroid Dehydrogenases
Gonadotrophs
Pregnenolone
Isomerases
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Oxygen concentration is an important factor for modulating progesterone synthesis in bovine corpus luteum. / Nishimura, Ryo; Sakumoto, Ryosuke; Tatsukawa, Yuko; Acosta, Tomas J.; Okuda, Kiyoshi.

In: Endocrinology, Vol. 147, No. 9, 2006, p. 4273-4280.

Research output: Contribution to journalArticle

Nishimura, R, Sakumoto, R, Tatsukawa, Y, Acosta, TJ & Okuda, K 2006, 'Oxygen concentration is an important factor for modulating progesterone synthesis in bovine corpus luteum', Endocrinology, vol. 147, no. 9, pp. 4273-4280. https://doi.org/10.1210/en.2005-1611
Nishimura, Ryo ; Sakumoto, Ryosuke ; Tatsukawa, Yuko ; Acosta, Tomas J. ; Okuda, Kiyoshi. / Oxygen concentration is an important factor for modulating progesterone synthesis in bovine corpus luteum. In: Endocrinology. 2006 ; Vol. 147, No. 9. pp. 4273-4280.
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