Oxidative stress in neonates: Evaluation using specific biomarkers

Hirokazu Tsukahara, Mi Zu Jiang, Naoko Ohta, Shuko Sato, Satoshi Tamura, Masahiro Hiraoka, Masayuki Maeda, Mitsufumi Mayumi

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Increased oxidative stress has been implicated in pathogenesis of serious diseases in neonates. We measured urinary levels of 8-hydroxy-2′- deoxyguanosine (a marker of oxidative DNA damage), acrolein-lysine adduct (a marker of lipid peroxidation and oxidative protein damage), and nitrite/nitrate (a marker of endogenous nitric oxide formation) in one-month-old neonates to examine the status of oxidative stress and its relationship to the degree of prematurity and clinical condition in neonates. Study subjects comprised three groups: healthy term neonates, clinically stable preterm neonates requiring no supplemental oxygen, and clinically sick preterm neonates requiring supplemental oxygen and ventilator support. Urinary levels of 8-hydroxy-2′- deoxyguanosine and acrolein-lysine adduct were significantly higher in sick preterm neonates than those of stable preterm and healthy term neonates. In the sick preterm group, neonates developing active retinopathy showed significantly higher levels of acrolein-lysine adduct than the other neonates without retinopathy. There were no significant differences in both urinary markers of oxidative stress between stable preterm and healthy term neonates. The urinary nitrite/nitrate levels were not significantly different among the three groups, suggesting no difference in endogenous nitric oxide formation. Collectively, these results provide evidence of augmentation of oxidative damage to DNA, lipids and proteins, especially in clinically sick preterm neonates.

Original languageEnglish
Pages (from-to)933-938
Number of pages6
JournalLife Sciences
Volume75
Issue number8
DOIs
Publication statusPublished - Jul 9 2004
Externally publishedYes

Fingerprint

Acrolein
Oxidative stress
Biomarkers
Lysine
Oxidative Stress
Nitrites
Nitrates
DNA Damage
Nitric Oxide
Oxygen
Lipids
DNA
Mechanical Ventilators
Lipid Peroxidation
Proteins
8-oxo-7-hydrodeoxyguanosine

Keywords

  • 8-Hydroxy-2′-deoxyguanosine
  • Acrolein-lysine adduct
  • Neonate
  • Nitric oxide
  • Oxidative stress

ASJC Scopus subject areas

  • Pharmacology

Cite this

Tsukahara, H., Jiang, M. Z., Ohta, N., Sato, S., Tamura, S., Hiraoka, M., ... Mayumi, M. (2004). Oxidative stress in neonates: Evaluation using specific biomarkers. Life Sciences, 75(8), 933-938. https://doi.org/10.1016/j.lfs.2004.01.025

Oxidative stress in neonates : Evaluation using specific biomarkers. / Tsukahara, Hirokazu; Jiang, Mi Zu; Ohta, Naoko; Sato, Shuko; Tamura, Satoshi; Hiraoka, Masahiro; Maeda, Masayuki; Mayumi, Mitsufumi.

In: Life Sciences, Vol. 75, No. 8, 09.07.2004, p. 933-938.

Research output: Contribution to journalArticle

Tsukahara, H, Jiang, MZ, Ohta, N, Sato, S, Tamura, S, Hiraoka, M, Maeda, M & Mayumi, M 2004, 'Oxidative stress in neonates: Evaluation using specific biomarkers', Life Sciences, vol. 75, no. 8, pp. 933-938. https://doi.org/10.1016/j.lfs.2004.01.025
Tsukahara, Hirokazu ; Jiang, Mi Zu ; Ohta, Naoko ; Sato, Shuko ; Tamura, Satoshi ; Hiraoka, Masahiro ; Maeda, Masayuki ; Mayumi, Mitsufumi. / Oxidative stress in neonates : Evaluation using specific biomarkers. In: Life Sciences. 2004 ; Vol. 75, No. 8. pp. 933-938.
@article{a1a2f1fc23f44c20954b541cf1076ee6,
title = "Oxidative stress in neonates: Evaluation using specific biomarkers",
abstract = "Increased oxidative stress has been implicated in pathogenesis of serious diseases in neonates. We measured urinary levels of 8-hydroxy-2′- deoxyguanosine (a marker of oxidative DNA damage), acrolein-lysine adduct (a marker of lipid peroxidation and oxidative protein damage), and nitrite/nitrate (a marker of endogenous nitric oxide formation) in one-month-old neonates to examine the status of oxidative stress and its relationship to the degree of prematurity and clinical condition in neonates. Study subjects comprised three groups: healthy term neonates, clinically stable preterm neonates requiring no supplemental oxygen, and clinically sick preterm neonates requiring supplemental oxygen and ventilator support. Urinary levels of 8-hydroxy-2′- deoxyguanosine and acrolein-lysine adduct were significantly higher in sick preterm neonates than those of stable preterm and healthy term neonates. In the sick preterm group, neonates developing active retinopathy showed significantly higher levels of acrolein-lysine adduct than the other neonates without retinopathy. There were no significant differences in both urinary markers of oxidative stress between stable preterm and healthy term neonates. The urinary nitrite/nitrate levels were not significantly different among the three groups, suggesting no difference in endogenous nitric oxide formation. Collectively, these results provide evidence of augmentation of oxidative damage to DNA, lipids and proteins, especially in clinically sick preterm neonates.",
keywords = "8-Hydroxy-2′-deoxyguanosine, Acrolein-lysine adduct, Neonate, Nitric oxide, Oxidative stress",
author = "Hirokazu Tsukahara and Jiang, {Mi Zu} and Naoko Ohta and Shuko Sato and Satoshi Tamura and Masahiro Hiraoka and Masayuki Maeda and Mitsufumi Mayumi",
year = "2004",
month = "7",
day = "9",
doi = "10.1016/j.lfs.2004.01.025",
language = "English",
volume = "75",
pages = "933--938",
journal = "Life Sciences",
issn = "0024-3205",
publisher = "Elsevier Inc.",
number = "8",

}

TY - JOUR

T1 - Oxidative stress in neonates

T2 - Evaluation using specific biomarkers

AU - Tsukahara, Hirokazu

AU - Jiang, Mi Zu

AU - Ohta, Naoko

AU - Sato, Shuko

AU - Tamura, Satoshi

AU - Hiraoka, Masahiro

AU - Maeda, Masayuki

AU - Mayumi, Mitsufumi

PY - 2004/7/9

Y1 - 2004/7/9

N2 - Increased oxidative stress has been implicated in pathogenesis of serious diseases in neonates. We measured urinary levels of 8-hydroxy-2′- deoxyguanosine (a marker of oxidative DNA damage), acrolein-lysine adduct (a marker of lipid peroxidation and oxidative protein damage), and nitrite/nitrate (a marker of endogenous nitric oxide formation) in one-month-old neonates to examine the status of oxidative stress and its relationship to the degree of prematurity and clinical condition in neonates. Study subjects comprised three groups: healthy term neonates, clinically stable preterm neonates requiring no supplemental oxygen, and clinically sick preterm neonates requiring supplemental oxygen and ventilator support. Urinary levels of 8-hydroxy-2′- deoxyguanosine and acrolein-lysine adduct were significantly higher in sick preterm neonates than those of stable preterm and healthy term neonates. In the sick preterm group, neonates developing active retinopathy showed significantly higher levels of acrolein-lysine adduct than the other neonates without retinopathy. There were no significant differences in both urinary markers of oxidative stress between stable preterm and healthy term neonates. The urinary nitrite/nitrate levels were not significantly different among the three groups, suggesting no difference in endogenous nitric oxide formation. Collectively, these results provide evidence of augmentation of oxidative damage to DNA, lipids and proteins, especially in clinically sick preterm neonates.

AB - Increased oxidative stress has been implicated in pathogenesis of serious diseases in neonates. We measured urinary levels of 8-hydroxy-2′- deoxyguanosine (a marker of oxidative DNA damage), acrolein-lysine adduct (a marker of lipid peroxidation and oxidative protein damage), and nitrite/nitrate (a marker of endogenous nitric oxide formation) in one-month-old neonates to examine the status of oxidative stress and its relationship to the degree of prematurity and clinical condition in neonates. Study subjects comprised three groups: healthy term neonates, clinically stable preterm neonates requiring no supplemental oxygen, and clinically sick preterm neonates requiring supplemental oxygen and ventilator support. Urinary levels of 8-hydroxy-2′- deoxyguanosine and acrolein-lysine adduct were significantly higher in sick preterm neonates than those of stable preterm and healthy term neonates. In the sick preterm group, neonates developing active retinopathy showed significantly higher levels of acrolein-lysine adduct than the other neonates without retinopathy. There were no significant differences in both urinary markers of oxidative stress between stable preterm and healthy term neonates. The urinary nitrite/nitrate levels were not significantly different among the three groups, suggesting no difference in endogenous nitric oxide formation. Collectively, these results provide evidence of augmentation of oxidative damage to DNA, lipids and proteins, especially in clinically sick preterm neonates.

KW - 8-Hydroxy-2′-deoxyguanosine

KW - Acrolein-lysine adduct

KW - Neonate

KW - Nitric oxide

KW - Oxidative stress

UR - http://www.scopus.com/inward/record.url?scp=2942557323&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2942557323&partnerID=8YFLogxK

U2 - 10.1016/j.lfs.2004.01.025

DO - 10.1016/j.lfs.2004.01.025

M3 - Article

C2 - 15193953

AN - SCOPUS:2942557323

VL - 75

SP - 933

EP - 938

JO - Life Sciences

JF - Life Sciences

SN - 0024-3205

IS - 8

ER -