Oxidative stress enhances granulocytic differentiation in HL 60 cells, an acute promyelocytic leukemia cell line

Tetsuya Ogino, Michitaka Ozaki, Akihiro Matsukawa

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

This paper studied the effects of physiologically available oxidants on HL 60 differentiation induced by all-trans retinoic acid (ATRA) or dimethyl sulfoxide (DMSO). Hydrogen peroxide (15 μM) and taurine chloramine (200 μM) induced HL 60 differentiation, which was detected by CD11b expression and superoxide production. Cd11b and p67phox mRNA expression was also augmented by these oxidants. In contrast, reducing chemicals, such as dithiothreitol, 2,3-dimercapto-1-propanol and N-acetylcysteine inhibited CD11b expression. Notably, DMSO inhibited methionine sulfoxide reductase activity, induced heme oxygenase-1 (ho-1) mRNA and enhanced oxidant-induced cell death, which indicated that DMSO intensified oxidative stress. After the addition of oxidants, ho-1 expression preceded the cd11b expression. Vicinal dithiol-reactive phenylarsine oxide (50 nM) also increased CD11b expression induced by DMSO or ATRA. These observations suggested that oxidative stress enhanced granulocytic differentiation of HL 60 cells and that leukaemic cell differentiation was affected by cellular redox status.

Original languageEnglish
Pages (from-to)1328-1337
Number of pages10
JournalFree Radical Research
Volume44
Issue number11
DOIs
Publication statusPublished - Nov 2010

Fingerprint

Acute Promyelocytic Leukemia
Oxidative stress
HL-60 Cells
Dimethyl Sulfoxide
Oxidants
Oxidative Stress
Cells
Cell Line
Heme Oxygenase-1
Tretinoin
Dimercaprol
Messenger RNA
Dithiothreitol
Acetylcysteine
Cell death
Superoxides
Hydrogen Peroxide
Oxidation-Reduction
Cell Differentiation
Cell Death

Keywords

  • Cell differentiation
  • leukemia
  • methionine sulfoxide
  • protein oxidation
  • thiol

ASJC Scopus subject areas

  • Biochemistry

Cite this

Oxidative stress enhances granulocytic differentiation in HL 60 cells, an acute promyelocytic leukemia cell line. / Ogino, Tetsuya; Ozaki, Michitaka; Matsukawa, Akihiro.

In: Free Radical Research, Vol. 44, No. 11, 11.2010, p. 1328-1337.

Research output: Contribution to journalArticle

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