Oxidative stress controlling agents are effective for small intestinal injuries induced by non-steroidal anti-inflammatory drugs

Yoshiyasu Kono, Seiji Kawano, Akinobu Takaki, Yasuyuki Shimomura, Masahiro Onji, Hisashi Ishikawa, Sakuma Takahashi, Joichiro Horii, Sayo Kobayashi, Daisuke Kawai, Kazuhide Yamamoto, Hiroyuki Okada

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background and Aim: Video-capsule endoscopy (VCE) has shown that intestinal ulcers are common in non-steroidal anti-inflammatory drugs (NSAIDs) users, although the mechanisms and management have not been clearly defined. To explore the contribution of oxidative stress and potential of anti-oxidants for NSAIDs-induced intestinal ulcers, we assessed human serum oxidative stress balance and the effect of anti-oxidants using a mouse model. Methods: A total of 30 NSAIDs users (17 aspirin and 13 non-aspirin users) received VCE. Serum reactive oxygen metabolite (d-ROM) and antioxidative OXY-adsorbent test (OXY) were measured. The indomethacin (IND)-induced mouse intestinal ulcer model was used to assess the effect of anti-oxidants. Eight-week-old mice were divided into four groups; control diet and diet including IND (N group), IND and L-carnitine (NC group), and IND and vitamin E (NE group). Results: Serum OXY levels among non-aspirin users were lower in the mucosal injuries positive group than the negative group (P < 0.05). In the mouse models, the degree of mucosal injuries was lower in NC and NE than N (P < 0.01). Serum d-ROM levels were lower in NC and NE than N (P < 0.01), and OXY levels were higher in NC than N and NE (P < 0.01). The degeneration of intestinal mitochondria was mild in NC and NE. The serum KC/CXCL-1 level and hepatic expression of the anti-oxidant molecule Gpx4 were lower in NC than N. Conclusions: Non-aspirin NSAID-induced intestinal ulcers are related to decreased anti-oxidative stress function. Anti-oxidants, especially L-carnitine, are good candidates for intestinal ulcers.

Original languageEnglish
Pages (from-to)136-145
Number of pages10
JournalJournal of Gastroenterology and Hepatology (Australia)
Volume32
Issue number1
DOIs
Publication statusPublished - Jan 1 2017

Fingerprint

Oxidants
Ulcer
Oxidative Stress
Anti-Inflammatory Agents
Indomethacin
Wounds and Injuries
Capsule Endoscopy
Serum
Pharmaceutical Preparations
Carnitine
Drug Users
Diet
Vitamin E
Aspirin
Mitochondria
Oxygen
Liver

Keywords

  • L-carnitine
  • mitochondria
  • non-steroidal anti-inflammatory drugs (NSAIDs)
  • oxidative stress
  • vitamin E

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

Cite this

Oxidative stress controlling agents are effective for small intestinal injuries induced by non-steroidal anti-inflammatory drugs. / Kono, Yoshiyasu; Kawano, Seiji; Takaki, Akinobu; Shimomura, Yasuyuki; Onji, Masahiro; Ishikawa, Hisashi; Takahashi, Sakuma; Horii, Joichiro; Kobayashi, Sayo; Kawai, Daisuke; Yamamoto, Kazuhide; Okada, Hiroyuki.

In: Journal of Gastroenterology and Hepatology (Australia), Vol. 32, No. 1, 01.01.2017, p. 136-145.

Research output: Contribution to journalArticle

Kono, Yoshiyasu ; Kawano, Seiji ; Takaki, Akinobu ; Shimomura, Yasuyuki ; Onji, Masahiro ; Ishikawa, Hisashi ; Takahashi, Sakuma ; Horii, Joichiro ; Kobayashi, Sayo ; Kawai, Daisuke ; Yamamoto, Kazuhide ; Okada, Hiroyuki. / Oxidative stress controlling agents are effective for small intestinal injuries induced by non-steroidal anti-inflammatory drugs. In: Journal of Gastroenterology and Hepatology (Australia). 2017 ; Vol. 32, No. 1. pp. 136-145.
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AU - Takaki, Akinobu

AU - Shimomura, Yasuyuki

AU - Onji, Masahiro

AU - Ishikawa, Hisashi

AU - Takahashi, Sakuma

AU - Horii, Joichiro

AU - Kobayashi, Sayo

AU - Kawai, Daisuke

AU - Yamamoto, Kazuhide

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KW - vitamin E

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