Oxidative stress can affect the gene silencing effect of DOTAP liposome in an In Vitro translation system

Hiroshi Umakoshi, Tomoyuki Tanabe, Keishi Suga, Huong Thi Bui, Toshinori Shimanouchi, Ryoichi Kuboi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Oxidative stress can affect in vitro GFP expression through its control of the gene silencing effect of the liposome prepared by 1,2-dioleoyl-3-trimethyl-ammonium propane (DOTAP). The gene silencing effect of cationic DOTAP liposome in in vitro GFP expression, especially focusing on its translation process, and the effects of oxidative stress on its silencing effect were investigated. GFP expression, initiated by mRNA, was found to be thoroughly inhibited in the presence of DOTAP liposome at concentration of more than 2.5 mM, though its in-hibitory effect was reduced in the presence of hydrogen peroxide. The analyses of (i) the interaction of mRNA with DOTAP, (ii) the chemical structure of DOTAP, and (iii) the membrane fluidity of DOTAP liposome imply the possible role of gene expression by the liposome membrane and stress conditions.

Original languageEnglish
Pages (from-to)253-260
Number of pages8
JournalInternational Journal of Biological Sciences
Volume7
Issue number3
DOIs
Publication statusPublished - Jan 1 2011
Externally publishedYes

Keywords

  • GFP expression
  • Gene silencing
  • Liposome
  • Oxidative stress

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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