Oxidative stress can affect the gene silencing effect of DOTAP liposome in an In Vitro translation system

Hiroshi Umakoshi, Tomoyuki Tanabe, Keishi Suga, Huong Thi Bui, Toshinori Shimanouchi, Ryoichi Kuboi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Oxidative stress can affect in vitro GFP expression through its control of the gene silencing effect of the liposome prepared by 1,2-dioleoyl-3-trimethyl-ammonium propane (DOTAP). The gene silencing effect of cationic DOTAP liposome in in vitro GFP expression, especially focusing on its translation process, and the effects of oxidative stress on its silencing effect were investigated. GFP expression, initiated by mRNA, was found to be thoroughly inhibited in the presence of DOTAP liposome at concentration of more than 2.5 mM, though its in-hibitory effect was reduced in the presence of hydrogen peroxide. The analyses of (i) the interaction of mRNA with DOTAP, (ii) the chemical structure of DOTAP, and (iii) the membrane fluidity of DOTAP liposome imply the possible role of gene expression by the liposome membrane and stress conditions.

Original languageEnglish
Pages (from-to)253-260
Number of pages8
JournalInternational Journal of Biological Sciences
Volume7
Issue number3
Publication statusPublished - 2011
Externally publishedYes

Fingerprint

Propane
propane
Gene Silencing
gene silencing
Ammonium Compounds
Liposomes
translation (genetics)
Oxidative Stress
oxidative stress
ammonium
gene
membrane
Messenger RNA
membrane fluidity
Membrane Fluidity
chemical structure
hydrogen peroxide
Hydrogen Peroxide
gene expression
In Vitro Techniques

Keywords

  • Gene silencing
  • GFP expression
  • Liposome
  • Oxidative stress

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics
  • Applied Microbiology and Biotechnology

Cite this

Oxidative stress can affect the gene silencing effect of DOTAP liposome in an In Vitro translation system. / Umakoshi, Hiroshi; Tanabe, Tomoyuki; Suga, Keishi; Bui, Huong Thi; Shimanouchi, Toshinori; Kuboi, Ryoichi.

In: International Journal of Biological Sciences, Vol. 7, No. 3, 2011, p. 253-260.

Research output: Contribution to journalArticle

Umakoshi, Hiroshi ; Tanabe, Tomoyuki ; Suga, Keishi ; Bui, Huong Thi ; Shimanouchi, Toshinori ; Kuboi, Ryoichi. / Oxidative stress can affect the gene silencing effect of DOTAP liposome in an In Vitro translation system. In: International Journal of Biological Sciences. 2011 ; Vol. 7, No. 3. pp. 253-260.
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