Abstract
Aluminum (Al) and ferrous iron [Fe(II)] are separately non-toxic to cultured tobacco cells in nutrient solution. However, Al and Fe(II) together cause the peroxidation of membrane lipids, the accumulation of Al and Fe, and the loss of viability [Ono et al. (1995) Plant Cell Physiol. 36: 115]. We investigated the cause-and-effect relationships of these various responses. In cells exposed to Fe(II) or Fe(III)-EDTA, both the peroxidation of lipids and the loss of viability were similarly enhanced by AlCl3 in a dose-dependent manner. During exposure to AlCl3, the accumulation of Al and the loss of viability became apparent rapidly and simultaneously at 8 h, whereas both the peroxidation of lipids and the accumulation of Fe occurred at later times. However, lipophilic antioxidants protected cells efficiently not only from the peroxidation of lipids but also from the loss of viability and the accumulation of Al and Fe. These results suggest that the peroxidation of lipids in the plasma membrane that is caused by both Al and Fe leads to the accumulation of Al and Fe and the loss of viability.
| Original language | English |
|---|---|
| Pages (from-to) | 1333-1339 |
| Number of pages | 7 |
| Journal | Plant and Cell Physiology |
| Volume | 38 |
| Issue number | 12 |
| Publication status | Published - Dec 1997 |
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Keywords
- Aluminum
- Antioxidants
- Iron
- Lipid peroxidation
- Nicotiana tabacum
ASJC Scopus subject areas
- Plant Science
- Cell Biology
- Physiology
Cite this
Oxidative damage to membranes by a combination of aluminum and iron in suspension-cultured tobacco cells. / Yamamoto, Yoko; Hachiya, Akiko; Matsumoto, Hideaki.
In: Plant and Cell Physiology, Vol. 38, No. 12, 12.1997, p. 1333-1339.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Oxidative damage to membranes by a combination of aluminum and iron in suspension-cultured tobacco cells
AU - Yamamoto, Yoko
AU - Hachiya, Akiko
AU - Matsumoto, Hideaki
PY - 1997/12
Y1 - 1997/12
N2 - Aluminum (Al) and ferrous iron [Fe(II)] are separately non-toxic to cultured tobacco cells in nutrient solution. However, Al and Fe(II) together cause the peroxidation of membrane lipids, the accumulation of Al and Fe, and the loss of viability [Ono et al. (1995) Plant Cell Physiol. 36: 115]. We investigated the cause-and-effect relationships of these various responses. In cells exposed to Fe(II) or Fe(III)-EDTA, both the peroxidation of lipids and the loss of viability were similarly enhanced by AlCl3 in a dose-dependent manner. During exposure to AlCl3, the accumulation of Al and the loss of viability became apparent rapidly and simultaneously at 8 h, whereas both the peroxidation of lipids and the accumulation of Fe occurred at later times. However, lipophilic antioxidants protected cells efficiently not only from the peroxidation of lipids but also from the loss of viability and the accumulation of Al and Fe. These results suggest that the peroxidation of lipids in the plasma membrane that is caused by both Al and Fe leads to the accumulation of Al and Fe and the loss of viability.
AB - Aluminum (Al) and ferrous iron [Fe(II)] are separately non-toxic to cultured tobacco cells in nutrient solution. However, Al and Fe(II) together cause the peroxidation of membrane lipids, the accumulation of Al and Fe, and the loss of viability [Ono et al. (1995) Plant Cell Physiol. 36: 115]. We investigated the cause-and-effect relationships of these various responses. In cells exposed to Fe(II) or Fe(III)-EDTA, both the peroxidation of lipids and the loss of viability were similarly enhanced by AlCl3 in a dose-dependent manner. During exposure to AlCl3, the accumulation of Al and the loss of viability became apparent rapidly and simultaneously at 8 h, whereas both the peroxidation of lipids and the accumulation of Fe occurred at later times. However, lipophilic antioxidants protected cells efficiently not only from the peroxidation of lipids but also from the loss of viability and the accumulation of Al and Fe. These results suggest that the peroxidation of lipids in the plasma membrane that is caused by both Al and Fe leads to the accumulation of Al and Fe and the loss of viability.
KW - Aluminum
KW - Antioxidants
KW - Iron
KW - Lipid peroxidation
KW - Nicotiana tabacum
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UR - http://www.scopus.com/inward/citedby.url?scp=0031439987&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0031439987
VL - 38
SP - 1333
EP - 1339
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 12
ER -