Oxidative damage to membranes by a combination of aluminum and iron in suspension-cultured tobacco cells

Yoko Yamamoto, Akiko Hachiya, Hideaki Matsumoto

Research output: Contribution to journalArticlepeer-review

99 Citations (Scopus)

Abstract

Aluminum (Al) and ferrous iron [Fe(II)] are separately non-toxic to cultured tobacco cells in nutrient solution. However, Al and Fe(II) together cause the peroxidation of membrane lipids, the accumulation of Al and Fe, and the loss of viability [Ono et al. (1995) Plant Cell Physiol. 36: 115]. We investigated the cause-and-effect relationships of these various responses. In cells exposed to Fe(II) or Fe(III)-EDTA, both the peroxidation of lipids and the loss of viability were similarly enhanced by AlCl3 in a dose-dependent manner. During exposure to AlCl3, the accumulation of Al and the loss of viability became apparent rapidly and simultaneously at 8 h, whereas both the peroxidation of lipids and the accumulation of Fe occurred at later times. However, lipophilic antioxidants protected cells efficiently not only from the peroxidation of lipids but also from the loss of viability and the accumulation of Al and Fe. These results suggest that the peroxidation of lipids in the plasma membrane that is caused by both Al and Fe leads to the accumulation of Al and Fe and the loss of viability.

Original languageEnglish
Pages (from-to)1333-1339
Number of pages7
JournalPlant and Cell Physiology
Volume38
Issue number12
DOIs
Publication statusPublished - Dec 1997

Keywords

  • Aluminum
  • Antioxidants
  • Iron
  • Lipid peroxidation
  • Nicotiana tabacum

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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