Oxidative damage and breakage of DNA in rat brain after transient MCA occlusion

Takeshi Hayashi, Masahiro Sakurai, Yasuto Itoyama, Koji Abe

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

As thrombolytic therapy for treatment of ischemic stroke was propagated, much attention has been paid to reperfusion brain injury. Oxidative stress is one of the most important factors that exacerbate tissue damage by reperfusion. Thus, we investigated the extent of oxidative damage in rat brain after transient middle cerebral artery (MCA) occlusion by immunohistochemical analysis for 8-hydroxy-2'-deoxyguanosine (8-OHdG), which is one of the best markers of oxidative damage. Furthermore, in order to investigate its role in neuronal cell death, we performed terminal deoxynucleotidyl transferase-mediated dUTP-biotin in situ nick end labeling (TUNEL) study, and compared the results with that of 8-OHdG immunohistochemistry. There was no immunoreactive 8-OHdG in sham-operated brain, but it became present in neurons of MCA territory at 3 h of reperfusion after 90-min ischemia. At 48 h after reperfusion, cerebral tissue of MCA territory was severely destroyed, and many cells in that area revealed TUNEL positivity. Some neurons in MCA territory showed mild immunoreactivity for 8-OHdG at that time, but it was strongest in neurons in the outer area of MCA territory. Those cells did not show TUNEL positivity, suggesting that 8- OHdG production is not necessarily followed by early cell death. Here, it was demonstrated that oxidative DNA damage occurs in more extended area than that where cell death is recognized. Although this damage does not cause early cell death, this might result in more prolonged cell dysfunction and eventual neuronal loss. Anti-oxidant therapy might be required for treatment of stroke in the future.

Original languageEnglish
Pages (from-to)159-163
Number of pages5
JournalBrain Research
Volume832
Issue number1-2
DOIs
Publication statusPublished - Jun 19 1999

Fingerprint

Middle Cerebral Artery Infarction
In Situ Nick-End Labeling
Middle Cerebral Artery
DNA Damage
Cell Death
Brain
Reperfusion Injury
Neurons
Reperfusion
Stroke
DNA Nucleotidylexotransferase
Thrombolytic Therapy
Thromboplastin
Biotin
Oxidants
Brain Injuries
Oxidative Stress
Therapeutics
Ischemia
Immunohistochemistry

Keywords

  • 8-Hydroxy-2'-deoxyguanosine
  • Cerebral ischemia
  • Oxidative stress
  • Rat
  • Terminal deoxynucleotidyl transferase-mediated dUTP-biotin in situ nick end labeling (TUNEL)

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Oxidative damage and breakage of DNA in rat brain after transient MCA occlusion. / Hayashi, Takeshi; Sakurai, Masahiro; Itoyama, Yasuto; Abe, Koji.

In: Brain Research, Vol. 832, No. 1-2, 19.06.1999, p. 159-163.

Research output: Contribution to journalArticle

Hayashi, Takeshi ; Sakurai, Masahiro ; Itoyama, Yasuto ; Abe, Koji. / Oxidative damage and breakage of DNA in rat brain after transient MCA occlusion. In: Brain Research. 1999 ; Vol. 832, No. 1-2. pp. 159-163.
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