Overexpression of UbcH10 alternates the cell cycle profile and accelerate the tumor proliferation in colon cancer

Takeo Fujita, Hirokuni Ikeda, Naruto Taira, Shinji Hatoh, Minoru Naito, Hiroyoshi Doihara

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Background: UbcH10 participates in proper metaphase to anaphase transition, and abrogation of UbcH10 results in the premature separation of sister chromatids. To assess the potential role of UbcH10 in colon cancer progression, we analyzed the clinicopathological relevance of UbcH10 in colon cancer. Methods: We firstly screened the expression profile of UbcH10 in various types of cancer tissues as well as cell lines. Thereafter, using the colon cancer cells line, we manipulated the expression of UbcH10 and evaluated the cell cycle profile and cellular proliferations. Furthermore, the clinicopathological significance of UbcH10 was immunohistologically evaluated in patients with colon cancer. Statistical analysis was performed using the student's t-test and Chi-square test. Results: Using the colon cancer cells, depletion of UbcH10 resulted in suppression of cellular growth whereas overexpression of UbcH10 promoted the cellular growth and oncogenic cellular growth. Mitotic population was markedly alternated by the manipulation of UbcH10 expression. Immunohistochemical analysis indicated that UbcH10 was significantly higher in colon cancer tissue compared with normal colon epithelia. Furthermore, the clinicopathological evaluation revealed that UbcH10 was associated with high-grade histological tumors. Conclusion: The results show the clinicopathological significance of UbcH10 in the progression of colon cancer. Thus UbcH10 may act as a novel biomarker in patients with colon cancer.

Original languageEnglish
Article number87
JournalBMC Cancer
Volume9
DOIs
Publication statusPublished - Mar 21 2009

Fingerprint

Colonic Neoplasms
Cell Cycle
Neoplasms
Growth
Cell Line
Anaphase
Chromatids
Chi-Square Distribution
Metaphase
Siblings
Colon
Epithelium
Biomarkers
Cell Proliferation
Students
Population

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Oncology

Cite this

Overexpression of UbcH10 alternates the cell cycle profile and accelerate the tumor proliferation in colon cancer. / Fujita, Takeo; Ikeda, Hirokuni; Taira, Naruto; Hatoh, Shinji; Naito, Minoru; Doihara, Hiroyoshi.

In: BMC Cancer, Vol. 9, 87, 21.03.2009.

Research output: Contribution to journalArticle

@article{67e27e884b4c44e1ae6b9e046318e8e0,
title = "Overexpression of UbcH10 alternates the cell cycle profile and accelerate the tumor proliferation in colon cancer",
abstract = "Background: UbcH10 participates in proper metaphase to anaphase transition, and abrogation of UbcH10 results in the premature separation of sister chromatids. To assess the potential role of UbcH10 in colon cancer progression, we analyzed the clinicopathological relevance of UbcH10 in colon cancer. Methods: We firstly screened the expression profile of UbcH10 in various types of cancer tissues as well as cell lines. Thereafter, using the colon cancer cells line, we manipulated the expression of UbcH10 and evaluated the cell cycle profile and cellular proliferations. Furthermore, the clinicopathological significance of UbcH10 was immunohistologically evaluated in patients with colon cancer. Statistical analysis was performed using the student's t-test and Chi-square test. Results: Using the colon cancer cells, depletion of UbcH10 resulted in suppression of cellular growth whereas overexpression of UbcH10 promoted the cellular growth and oncogenic cellular growth. Mitotic population was markedly alternated by the manipulation of UbcH10 expression. Immunohistochemical analysis indicated that UbcH10 was significantly higher in colon cancer tissue compared with normal colon epithelia. Furthermore, the clinicopathological evaluation revealed that UbcH10 was associated with high-grade histological tumors. Conclusion: The results show the clinicopathological significance of UbcH10 in the progression of colon cancer. Thus UbcH10 may act as a novel biomarker in patients with colon cancer.",
author = "Takeo Fujita and Hirokuni Ikeda and Naruto Taira and Shinji Hatoh and Minoru Naito and Hiroyoshi Doihara",
year = "2009",
month = "3",
day = "21",
doi = "10.1186/1471-2407-9-87",
language = "English",
volume = "9",
journal = "BMC Cancer",
issn = "1471-2407",
publisher = "BioMed Central",

}

TY - JOUR

T1 - Overexpression of UbcH10 alternates the cell cycle profile and accelerate the tumor proliferation in colon cancer

AU - Fujita, Takeo

AU - Ikeda, Hirokuni

AU - Taira, Naruto

AU - Hatoh, Shinji

AU - Naito, Minoru

AU - Doihara, Hiroyoshi

PY - 2009/3/21

Y1 - 2009/3/21

N2 - Background: UbcH10 participates in proper metaphase to anaphase transition, and abrogation of UbcH10 results in the premature separation of sister chromatids. To assess the potential role of UbcH10 in colon cancer progression, we analyzed the clinicopathological relevance of UbcH10 in colon cancer. Methods: We firstly screened the expression profile of UbcH10 in various types of cancer tissues as well as cell lines. Thereafter, using the colon cancer cells line, we manipulated the expression of UbcH10 and evaluated the cell cycle profile and cellular proliferations. Furthermore, the clinicopathological significance of UbcH10 was immunohistologically evaluated in patients with colon cancer. Statistical analysis was performed using the student's t-test and Chi-square test. Results: Using the colon cancer cells, depletion of UbcH10 resulted in suppression of cellular growth whereas overexpression of UbcH10 promoted the cellular growth and oncogenic cellular growth. Mitotic population was markedly alternated by the manipulation of UbcH10 expression. Immunohistochemical analysis indicated that UbcH10 was significantly higher in colon cancer tissue compared with normal colon epithelia. Furthermore, the clinicopathological evaluation revealed that UbcH10 was associated with high-grade histological tumors. Conclusion: The results show the clinicopathological significance of UbcH10 in the progression of colon cancer. Thus UbcH10 may act as a novel biomarker in patients with colon cancer.

AB - Background: UbcH10 participates in proper metaphase to anaphase transition, and abrogation of UbcH10 results in the premature separation of sister chromatids. To assess the potential role of UbcH10 in colon cancer progression, we analyzed the clinicopathological relevance of UbcH10 in colon cancer. Methods: We firstly screened the expression profile of UbcH10 in various types of cancer tissues as well as cell lines. Thereafter, using the colon cancer cells line, we manipulated the expression of UbcH10 and evaluated the cell cycle profile and cellular proliferations. Furthermore, the clinicopathological significance of UbcH10 was immunohistologically evaluated in patients with colon cancer. Statistical analysis was performed using the student's t-test and Chi-square test. Results: Using the colon cancer cells, depletion of UbcH10 resulted in suppression of cellular growth whereas overexpression of UbcH10 promoted the cellular growth and oncogenic cellular growth. Mitotic population was markedly alternated by the manipulation of UbcH10 expression. Immunohistochemical analysis indicated that UbcH10 was significantly higher in colon cancer tissue compared with normal colon epithelia. Furthermore, the clinicopathological evaluation revealed that UbcH10 was associated with high-grade histological tumors. Conclusion: The results show the clinicopathological significance of UbcH10 in the progression of colon cancer. Thus UbcH10 may act as a novel biomarker in patients with colon cancer.

UR - http://www.scopus.com/inward/record.url?scp=64749096026&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=64749096026&partnerID=8YFLogxK

U2 - 10.1186/1471-2407-9-87

DO - 10.1186/1471-2407-9-87

M3 - Article

C2 - 19302711

AN - SCOPUS:64749096026

VL - 9

JO - BMC Cancer

JF - BMC Cancer

SN - 1471-2407

M1 - 87

ER -