Abstract
Expression of the Wilms' tumor gene WTI in de novo lung cancer was examined using quantitative real-time RT-PCR and immunohistochemistry. Overexpression of the WTI gene was detected by RT-PCR in 54/56 (96%) de novo non-small cell lung cancers examined and confirmed by detection of WTI protein with an anti-WTI antibody. Overexpression of the WTI gene was also demonstrated in 5/6 (83%) de novo small cell lung cancers by immunohistochemistry. Furthermore, when the WTI gene was examined for mutations by direct sequencing of genomic DNA in 7 lung cancers, no mutations were found. These results suggest that the non-mutated, wild-type WTI gene plays an important role in tumorigenesis of de novo lung cancers and may provide us with the rationale for new therapeutic strategies for lung cancer targeting the WTI gene and its products.
| Original language | English |
|---|---|
| Pages (from-to) | 297-303 |
| Number of pages | 7 |
| Journal | International Journal of Cancer |
| Volume | 100 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jul 20 2002 |
| Externally published | Yes |
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Keywords
- Lung cancer
- Solid tumor
- Tumorigenesis
- Wilms' tumor
- WTI
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Overexpression of the Wilms' tumor gene WT1 in de novo lung cancers. / Oji, Yusuke; Miyoshi, Shinichiro; Maeda, Hajime; Hayashi, Seiji; Tamaki, Hiroya; Nakatsuka, Shin Ichi; Yao, Masayuki; Takahashi, Eigo; Nakano, Yoko; Hirabayashi, Hirohisa; Shintani, Yasushi; Oka, Yoshihiro; Tsuboi, Akihiro; Hosen, Naoki; Asada, Momotaro; Fujioka, Tatsuya; Murakami, Masaki; Kanato, Keisuke; Motomura, Mari; Kim, Eui Ho; Kawakami, Manabu; Ikegame, Kazuhiro; Ogawa, Hiroyasu; Aozasa, Katsuyuki; Kawase, Ichiro; Sugiyama, Haruo.
In: International Journal of Cancer, Vol. 100, No. 3, 20.07.2002, p. 297-303.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Overexpression of the Wilms' tumor gene WT1 in de novo lung cancers
AU - Oji, Yusuke
AU - Miyoshi, Shinichiro
AU - Maeda, Hajime
AU - Hayashi, Seiji
AU - Tamaki, Hiroya
AU - Nakatsuka, Shin Ichi
AU - Yao, Masayuki
AU - Takahashi, Eigo
AU - Nakano, Yoko
AU - Hirabayashi, Hirohisa
AU - Shintani, Yasushi
AU - Oka, Yoshihiro
AU - Tsuboi, Akihiro
AU - Hosen, Naoki
AU - Asada, Momotaro
AU - Fujioka, Tatsuya
AU - Murakami, Masaki
AU - Kanato, Keisuke
AU - Motomura, Mari
AU - Kim, Eui Ho
AU - Kawakami, Manabu
AU - Ikegame, Kazuhiro
AU - Ogawa, Hiroyasu
AU - Aozasa, Katsuyuki
AU - Kawase, Ichiro
AU - Sugiyama, Haruo
PY - 2002/7/20
Y1 - 2002/7/20
N2 - Expression of the Wilms' tumor gene WTI in de novo lung cancer was examined using quantitative real-time RT-PCR and immunohistochemistry. Overexpression of the WTI gene was detected by RT-PCR in 54/56 (96%) de novo non-small cell lung cancers examined and confirmed by detection of WTI protein with an anti-WTI antibody. Overexpression of the WTI gene was also demonstrated in 5/6 (83%) de novo small cell lung cancers by immunohistochemistry. Furthermore, when the WTI gene was examined for mutations by direct sequencing of genomic DNA in 7 lung cancers, no mutations were found. These results suggest that the non-mutated, wild-type WTI gene plays an important role in tumorigenesis of de novo lung cancers and may provide us with the rationale for new therapeutic strategies for lung cancer targeting the WTI gene and its products.
AB - Expression of the Wilms' tumor gene WTI in de novo lung cancer was examined using quantitative real-time RT-PCR and immunohistochemistry. Overexpression of the WTI gene was detected by RT-PCR in 54/56 (96%) de novo non-small cell lung cancers examined and confirmed by detection of WTI protein with an anti-WTI antibody. Overexpression of the WTI gene was also demonstrated in 5/6 (83%) de novo small cell lung cancers by immunohistochemistry. Furthermore, when the WTI gene was examined for mutations by direct sequencing of genomic DNA in 7 lung cancers, no mutations were found. These results suggest that the non-mutated, wild-type WTI gene plays an important role in tumorigenesis of de novo lung cancers and may provide us with the rationale for new therapeutic strategies for lung cancer targeting the WTI gene and its products.
KW - Lung cancer
KW - Solid tumor
KW - Tumorigenesis
KW - Wilms' tumor
KW - WTI
UR - http://www.scopus.com/inward/record.url?scp=0037143087&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037143087&partnerID=8YFLogxK
U2 - 10.1002/ijc.10476
DO - 10.1002/ijc.10476
M3 - Article
C2 - 12115544
AN - SCOPUS:0037143087
VL - 100
SP - 297
EP - 303
JO - International Journal of Cancer
JF - International Journal of Cancer
SN - 0020-7136
IS - 3
ER -