Overexpression of the Wilms' tumor gene WT1 in de novo lung cancers

Yusuke Oji, Shinichiro Miyoshi, Hajime Maeda, Seiji Hayashi, Hiroya Tamaki, Shin Ichi Nakatsuka, Masayuki Yao, Eigo Takahashi, Yoko Nakano, Hirohisa Hirabayashi, Yasushi Shintani, Yoshihiro Oka, Akihiro Tsuboi, Naoki Hosen, Momotaro Asada, Tatsuya Fujioka, Masaki Murakami, Keisuke Kanato, Mari Motomura, Eui Ho KimManabu Kawakami, Kazuhiro Ikegame, Hiroyasu Ogawa, Katsuyuki Aozasa, Ichiro Kawase, Haruo Sugiyama

Research output: Contribution to journalArticle

175 Citations (Scopus)

Abstract

Expression of the Wilms' tumor gene WTI in de novo lung cancer was examined using quantitative real-time RT-PCR and immunohistochemistry. Overexpression of the WTI gene was detected by RT-PCR in 54/56 (96%) de novo non-small cell lung cancers examined and confirmed by detection of WTI protein with an anti-WTI antibody. Overexpression of the WTI gene was also demonstrated in 5/6 (83%) de novo small cell lung cancers by immunohistochemistry. Furthermore, when the WTI gene was examined for mutations by direct sequencing of genomic DNA in 7 lung cancers, no mutations were found. These results suggest that the non-mutated, wild-type WTI gene plays an important role in tumorigenesis of de novo lung cancers and may provide us with the rationale for new therapeutic strategies for lung cancer targeting the WTI gene and its products.

Original languageEnglish
Pages (from-to)297-303
Number of pages7
JournalInternational Journal of Cancer
Volume100
Issue number3
DOIs
Publication statusPublished - Jul 20 2002

Keywords

  • Lung cancer
  • Solid tumor
  • Tumorigenesis
  • WTI
  • Wilms' tumor

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Oji, Y., Miyoshi, S., Maeda, H., Hayashi, S., Tamaki, H., Nakatsuka, S. I., Yao, M., Takahashi, E., Nakano, Y., Hirabayashi, H., Shintani, Y., Oka, Y., Tsuboi, A., Hosen, N., Asada, M., Fujioka, T., Murakami, M., Kanato, K., Motomura, M., ... Sugiyama, H. (2002). Overexpression of the Wilms' tumor gene WT1 in de novo lung cancers. International Journal of Cancer, 100(3), 297-303. https://doi.org/10.1002/ijc.10476