Overexpression of the Wilms' tumor gene WT1 in de novo lung cancers

Yusuke Oji; Shinichiro Miyoshi; Hajime Maeda; Seiji Hayashi; Hiroya Tamaki; Shin Ichi Nakatsuka; Masayuki Yao; Eigo Takahashi; Yoko Nakano; Hirohisa Hirabayashi; Yasushi Shintani; Yoshihiro Oka; Akihiro Tsuboi; Naoki Hosen; Momotaro Asada; Tatsuya Fujioka; Masaki Murakami; Keisuke Kanato; Mari Motomura; Eui Ho Kim; Manabu Kawakami; Kazuhiro Ikegame; Hiroyasu Ogawa; Katsuyuki Aozasa; Ichiro Kawase; Haruo Sugiyama

doi:10.1002/ijc.10476

Overexpression of the Wilms' tumor gene WT1 in de novo lung cancers

Yusuke Oji, Shinichiro Miyoshi, Hajime Maeda, Seiji Hayashi, Hiroya Tamaki, Shin Ichi Nakatsuka, Masayuki Yao, Eigo Takahashi, Yoko Nakano, Hirohisa Hirabayashi, Yasushi Shintani, Yoshihiro Oka, Akihiro Tsuboi, Naoki Hosen, Momotaro Asada, Tatsuya Fujioka, Masaki Murakami, Keisuke Kanato, Mari Motomura, Eui Ho KimManabu Kawakami, Kazuhiro Ikegame, Hiroyasu Ogawa, Katsuyuki Aozasa, Ichiro Kawase, Haruo Sugiyama

Graduate School of Medicine Dentistry and Pharmaceutical Sciences

Research output: Contribution to journal › Article

175 Citations (Scopus)

Abstract

Expression of the Wilms' tumor gene WTI in de novo lung cancer was examined using quantitative real-time RT-PCR and immunohistochemistry. Overexpression of the WTI gene was detected by RT-PCR in 54/56 (96%) de novo non-small cell lung cancers examined and confirmed by detection of WTI protein with an anti-WTI antibody. Overexpression of the WTI gene was also demonstrated in 5/6 (83%) de novo small cell lung cancers by immunohistochemistry. Furthermore, when the WTI gene was examined for mutations by direct sequencing of genomic DNA in 7 lung cancers, no mutations were found. These results suggest that the non-mutated, wild-type WTI gene plays an important role in tumorigenesis of de novo lung cancers and may provide us with the rationale for new therapeutic strategies for lung cancer targeting the WTI gene and its products.

Original language	English
Pages (from-to)	297-303
Number of pages	7
Journal	International Journal of Cancer
Volume	100
Issue number	3
DOIs	https://doi.org/10.1002/ijc.10476
Publication status	Published - Jul 20 2002

Keywords

Lung cancer
Solid tumor
Tumorigenesis
WTI
Wilms' tumor

ASJC Scopus subject areas

Oncology
Cancer Research

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Oji, Y., Miyoshi, S., Maeda, H., Hayashi, S., Tamaki, H., Nakatsuka, S. I., Yao, M., Takahashi, E., Nakano, Y., Hirabayashi, H., Shintani, Y., Oka, Y., Tsuboi, A., Hosen, N., Asada, M., Fujioka, T., Murakami, M., Kanato, K., Motomura, M., ... Sugiyama, H. (2002). Overexpression of the Wilms' tumor gene WT1 in de novo lung cancers. International Journal of Cancer, 100(3), 297-303. https://doi.org/10.1002/ijc.10476

Overexpression of the Wilms' tumor gene WT1 in de novo lung cancers. / Oji, Yusuke; Miyoshi, Shinichiro; Maeda, Hajime; Hayashi, Seiji; Tamaki, Hiroya; Nakatsuka, Shin Ichi; Yao, Masayuki; Takahashi, Eigo; Nakano, Yoko; Hirabayashi, Hirohisa; Shintani, Yasushi; Oka, Yoshihiro; Tsuboi, Akihiro; Hosen, Naoki; Asada, Momotaro; Fujioka, Tatsuya; Murakami, Masaki; Kanato, Keisuke; Motomura, Mari; Kim, Eui Ho; Kawakami, Manabu; Ikegame, Kazuhiro; Ogawa, Hiroyasu; Aozasa, Katsuyuki; Kawase, Ichiro; Sugiyama, Haruo.

In: International Journal of Cancer, Vol. 100, No. 3, 20.07.2002, p. 297-303.

Research output: Contribution to journal › Article

Oji, Y, Miyoshi, S, Maeda, H, Hayashi, S, Tamaki, H, Nakatsuka, SI, Yao, M, Takahashi, E, Nakano, Y, Hirabayashi, H, Shintani, Y, Oka, Y, Tsuboi, A, Hosen, N, Asada, M, Fujioka, T, Murakami, M, Kanato, K, Motomura, M, Kim, EH, Kawakami, M, Ikegame, K, Ogawa, H, Aozasa, K, Kawase, I & Sugiyama, H 2002, 'Overexpression of the Wilms' tumor gene WT1 in de novo lung cancers', International Journal of Cancer, vol. 100, no. 3, pp. 297-303. https://doi.org/10.1002/ijc.10476

Oji, Yusuke ; Miyoshi, Shinichiro ; Maeda, Hajime ; Hayashi, Seiji ; Tamaki, Hiroya ; Nakatsuka, Shin Ichi ; Yao, Masayuki ; Takahashi, Eigo ; Nakano, Yoko ; Hirabayashi, Hirohisa ; Shintani, Yasushi ; Oka, Yoshihiro ; Tsuboi, Akihiro ; Hosen, Naoki ; Asada, Momotaro ; Fujioka, Tatsuya ; Murakami, Masaki ; Kanato, Keisuke ; Motomura, Mari ; Kim, Eui Ho ; Kawakami, Manabu ; Ikegame, Kazuhiro ; Ogawa, Hiroyasu ; Aozasa, Katsuyuki ; Kawase, Ichiro ; Sugiyama, Haruo. / Overexpression of the Wilms' tumor gene WT1 in de novo lung cancers. In: International Journal of Cancer. 2002 ; Vol. 100, No. 3. pp. 297-303.

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abstract = "Expression of the Wilms' tumor gene WTI in de novo lung cancer was examined using quantitative real-time RT-PCR and immunohistochemistry. Overexpression of the WTI gene was detected by RT-PCR in 54/56 (96%) de novo non-small cell lung cancers examined and confirmed by detection of WTI protein with an anti-WTI antibody. Overexpression of the WTI gene was also demonstrated in 5/6 (83%) de novo small cell lung cancers by immunohistochemistry. Furthermore, when the WTI gene was examined for mutations by direct sequencing of genomic DNA in 7 lung cancers, no mutations were found. These results suggest that the non-mutated, wild-type WTI gene plays an important role in tumorigenesis of de novo lung cancers and may provide us with the rationale for new therapeutic strategies for lung cancer targeting the WTI gene and its products.",

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AU - Fujioka, Tatsuya

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AU - Motomura, Mari

AU - Kim, Eui Ho

AU - Kawakami, Manabu

AU - Ikegame, Kazuhiro

AU - Ogawa, Hiroyasu

AU - Aozasa, Katsuyuki

AU - Kawase, Ichiro

AU - Sugiyama, Haruo

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