Overexpression of the Wilms' tumor gene WT1 in de novo lung cancers

Yusuke Oji; Shinichiro Miyoshi; Hajime Maeda; Seiji Hayashi; Hiroya Tamaki; Shin Ichi Nakatsuka; Masayuki Yao; Eigo Takahashi; Yoko Nakano; Hirohisa Hirabayashi; Yasushi Shintani; Yoshihiro Oka; Akihiro Tsuboi; Naoki Hosen; Momotaro Asada; Tatsuya Fujioka; Masaki Murakami; Keisuke Kanato; Mari Motomura; Eui Ho Kim; Manabu Kawakami; Kazuhiro Ikegame; Hiroyasu Ogawa; Katsuyuki Aozasa; Ichiro Kawase; Haruo Sugiyama

doi:10.1002/ijc.10476

Overexpression of the Wilms' tumor gene WT1 in de novo lung cancers

Yusuke Oji, Shinichiro Miyoshi, Hajime Maeda, Seiji Hayashi, Hiroya Tamaki, Shin Ichi Nakatsuka, Masayuki Yao, Eigo Takahashi, Yoko Nakano, Hirohisa Hirabayashi, Yasushi Shintani, Yoshihiro Oka, Akihiro Tsuboi, Naoki Hosen, Momotaro Asada, Tatsuya Fujioka, Masaki Murakami, Keisuke Kanato, Mari Motomura, Eui Ho KimManabu Kawakami, Kazuhiro Ikegame, Hiroyasu Ogawa, Katsuyuki Aozasa, Ichiro Kawase, Haruo Sugiyama

Graduate School of Medicine Dentistry and Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

192 Citations (Scopus)

Abstract

Expression of the Wilms' tumor gene WTI in de novo lung cancer was examined using quantitative real-time RT-PCR and immunohistochemistry. Overexpression of the WTI gene was detected by RT-PCR in 54/56 (96%) de novo non-small cell lung cancers examined and confirmed by detection of WTI protein with an anti-WTI antibody. Overexpression of the WTI gene was also demonstrated in 5/6 (83%) de novo small cell lung cancers by immunohistochemistry. Furthermore, when the WTI gene was examined for mutations by direct sequencing of genomic DNA in 7 lung cancers, no mutations were found. These results suggest that the non-mutated, wild-type WTI gene plays an important role in tumorigenesis of de novo lung cancers and may provide us with the rationale for new therapeutic strategies for lung cancer targeting the WTI gene and its products.

Original language	English
Pages (from-to)	297-303
Number of pages	7
Journal	International Journal of Cancer
Volume	100
Issue number	3
DOIs	https://doi.org/10.1002/ijc.10476
Publication status	Published - Jul 20 2002

Keywords

Lung cancer
Solid tumor
Tumorigenesis
WTI
Wilms' tumor

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Oji, Y., Miyoshi, S., Maeda, H., Hayashi, S., Tamaki, H., Nakatsuka, S. I., Yao, M., Takahashi, E., Nakano, Y., Hirabayashi, H., Shintani, Y., Oka, Y., Tsuboi, A., Hosen, N., Asada, M., Fujioka, T., Murakami, M., Kanato, K., Motomura, M., ... Sugiyama, H. (2002). Overexpression of the Wilms' tumor gene WT1 in de novo lung cancers. International Journal of Cancer, 100(3), 297-303. https://doi.org/10.1002/ijc.10476

Oji, Y, Miyoshi, S, Maeda, H, Hayashi, S, Tamaki, H, Nakatsuka, SI, Yao, M, Takahashi, E, Nakano, Y, Hirabayashi, H, Shintani, Y, Oka, Y, Tsuboi, A, Hosen, N, Asada, M, Fujioka, T, Murakami, M, Kanato, K, Motomura, M, Kim, EH, Kawakami, M, Ikegame, K, Ogawa, H, Aozasa, K, Kawase, I & Sugiyama, H 2002, 'Overexpression of the Wilms' tumor gene WT1 in de novo lung cancers', International Journal of Cancer, vol. 100, no. 3, pp. 297-303. https://doi.org/10.1002/ijc.10476

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title = "Overexpression of the Wilms' tumor gene WT1 in de novo lung cancers",

abstract = "Expression of the Wilms' tumor gene WTI in de novo lung cancer was examined using quantitative real-time RT-PCR and immunohistochemistry. Overexpression of the WTI gene was detected by RT-PCR in 54/56 (96%) de novo non-small cell lung cancers examined and confirmed by detection of WTI protein with an anti-WTI antibody. Overexpression of the WTI gene was also demonstrated in 5/6 (83%) de novo small cell lung cancers by immunohistochemistry. Furthermore, when the WTI gene was examined for mutations by direct sequencing of genomic DNA in 7 lung cancers, no mutations were found. These results suggest that the non-mutated, wild-type WTI gene plays an important role in tumorigenesis of de novo lung cancers and may provide us with the rationale for new therapeutic strategies for lung cancer targeting the WTI gene and its products.",

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author = "Yusuke Oji and Shinichiro Miyoshi and Hajime Maeda and Seiji Hayashi and Hiroya Tamaki and Nakatsuka, {Shin Ichi} and Masayuki Yao and Eigo Takahashi and Yoko Nakano and Hirohisa Hirabayashi and Yasushi Shintani and Yoshihiro Oka and Akihiro Tsuboi and Naoki Hosen and Momotaro Asada and Tatsuya Fujioka and Masaki Murakami and Keisuke Kanato and Mari Motomura and Kim, {Eui Ho} and Manabu Kawakami and Kazuhiro Ikegame and Hiroyasu Ogawa and Katsuyuki Aozasa and Ichiro Kawase and Haruo Sugiyama",

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AU - Oji, Yusuke

AU - Miyoshi, Shinichiro

AU - Maeda, Hajime

AU - Hayashi, Seiji

AU - Tamaki, Hiroya

AU - Nakatsuka, Shin Ichi

AU - Yao, Masayuki

AU - Takahashi, Eigo

AU - Nakano, Yoko

AU - Hirabayashi, Hirohisa

AU - Shintani, Yasushi

AU - Oka, Yoshihiro

AU - Tsuboi, Akihiro

AU - Hosen, Naoki

AU - Asada, Momotaro

AU - Fujioka, Tatsuya

AU - Murakami, Masaki

AU - Kanato, Keisuke

AU - Motomura, Mari

AU - Kim, Eui Ho

AU - Kawakami, Manabu

AU - Ikegame, Kazuhiro

AU - Ogawa, Hiroyasu

AU - Aozasa, Katsuyuki

AU - Kawase, Ichiro

AU - Sugiyama, Haruo

PY - 2002/7/20

Y1 - 2002/7/20

N2 - Expression of the Wilms' tumor gene WTI in de novo lung cancer was examined using quantitative real-time RT-PCR and immunohistochemistry. Overexpression of the WTI gene was detected by RT-PCR in 54/56 (96%) de novo non-small cell lung cancers examined and confirmed by detection of WTI protein with an anti-WTI antibody. Overexpression of the WTI gene was also demonstrated in 5/6 (83%) de novo small cell lung cancers by immunohistochemistry. Furthermore, when the WTI gene was examined for mutations by direct sequencing of genomic DNA in 7 lung cancers, no mutations were found. These results suggest that the non-mutated, wild-type WTI gene plays an important role in tumorigenesis of de novo lung cancers and may provide us with the rationale for new therapeutic strategies for lung cancer targeting the WTI gene and its products.

AB - Expression of the Wilms' tumor gene WTI in de novo lung cancer was examined using quantitative real-time RT-PCR and immunohistochemistry. Overexpression of the WTI gene was detected by RT-PCR in 54/56 (96%) de novo non-small cell lung cancers examined and confirmed by detection of WTI protein with an anti-WTI antibody. Overexpression of the WTI gene was also demonstrated in 5/6 (83%) de novo small cell lung cancers by immunohistochemistry. Furthermore, when the WTI gene was examined for mutations by direct sequencing of genomic DNA in 7 lung cancers, no mutations were found. These results suggest that the non-mutated, wild-type WTI gene plays an important role in tumorigenesis of de novo lung cancers and may provide us with the rationale for new therapeutic strategies for lung cancer targeting the WTI gene and its products.

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KW - Solid tumor

KW - Tumorigenesis

KW - WTI

KW - Wilms' tumor

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Overexpression of the Wilms' tumor gene WT1 in de novo lung cancers

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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