Abstract
Expression of the Wilms' tumor gene WTI in de novo lung cancer was examined using quantitative real-time RT-PCR and immunohistochemistry. Overexpression of the WTI gene was detected by RT-PCR in 54/56 (96%) de novo non-small cell lung cancers examined and confirmed by detection of WTI protein with an anti-WTI antibody. Overexpression of the WTI gene was also demonstrated in 5/6 (83%) de novo small cell lung cancers by immunohistochemistry. Furthermore, when the WTI gene was examined for mutations by direct sequencing of genomic DNA in 7 lung cancers, no mutations were found. These results suggest that the non-mutated, wild-type WTI gene plays an important role in tumorigenesis of de novo lung cancers and may provide us with the rationale for new therapeutic strategies for lung cancer targeting the WTI gene and its products.
Original language | English |
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Pages (from-to) | 297-303 |
Number of pages | 7 |
Journal | International Journal of Cancer |
Volume | 100 |
Issue number | 3 |
DOIs | |
Publication status | Published - Jul 20 2002 |
Keywords
- Lung cancer
- Solid tumor
- Tumorigenesis
- WTI
- Wilms' tumor
ASJC Scopus subject areas
- Oncology
- Cancer Research