Overexpression of the barley aquaporin HvPIP2;1 increases internal CO 2 conductance and CO2 assimilation in the leaves of transgenic rice plants

Yuko T. Hanba, Mineo Shibasaka, Yasuyuki Hayashi, Takahiko Hayakawa, Kunihiro Kasamo, Ichiro Terashima, Maki Katsuhara

Research output: Contribution to journalArticle

278 Citations (Scopus)

Abstract

The internal conductance for CO2 diffusion (gi) and CO2 assimilation rate were measured and the related anatomical characteristics were investigated in transgenic rice leaves that overexpressed barley aquaporin HvPIP2;1. This study was performed to test the hypothesis that aquaporin facilitates CO2 diffusion within leaves. The gi value was estimated for intact leaves by concurrent measurements of gas exchange and carbon isotope ratio. The leaves of the transgenic rice plants that expressed the highest levels of Aq-anti-HvPIP2;1 showed a 40% increase in g i as compared to gi in the leaves of wild-type rice plants. The increase in gi was accompanied by a 14% increase in CO2 assimilation rate and a 27% increase in stomatal conductance (gs). The transgenic plants that had low levels of Aq-anti-HvPIP2;1 showed decreases in gi and CO2 assimilation rate. In the plants with high levels of Aq-anti-HvPIP2;1, mesophyll cell size decreased and the cell walls of the epidermis and mesophyll cells thickened, indicating that the leaves had become xeromorphic. Although such anatomical changes could partially offset the increase in gi by the aquaporin, the increase in aquaporin content overcame such adverse effects.

Original languageEnglish
Pages (from-to)521-529
Number of pages9
JournalPlant and Cell Physiology
Volume45
Issue number5
DOIs
Publication statusPublished - May 2004

Fingerprint

Aquaporin 1
Aquaporins
aquaporins
Genetically Modified Plants
Hordeum
Carbon Monoxide
Mesophyll Cells
assimilation (physiology)
barley
genetically modified organisms
rice
Carbon Isotopes
leaves
Cell Size
Epidermis
mesophyll
Cell Wall
Gases
epidermis (plant)
gas exchange

Keywords

  • Aquaporin
  • CO assimilation rate
  • CO conductance
  • HvPIP2;1
  • Mesophyll anatomy
  • Stomatal
  • Transgenic rice

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Overexpression of the barley aquaporin HvPIP2;1 increases internal CO 2 conductance and CO2 assimilation in the leaves of transgenic rice plants. / Hanba, Yuko T.; Shibasaka, Mineo; Hayashi, Yasuyuki; Hayakawa, Takahiko; Kasamo, Kunihiro; Terashima, Ichiro; Katsuhara, Maki.

In: Plant and Cell Physiology, Vol. 45, No. 5, 05.2004, p. 521-529.

Research output: Contribution to journalArticle

Hanba, Yuko T. ; Shibasaka, Mineo ; Hayashi, Yasuyuki ; Hayakawa, Takahiko ; Kasamo, Kunihiro ; Terashima, Ichiro ; Katsuhara, Maki. / Overexpression of the barley aquaporin HvPIP2;1 increases internal CO 2 conductance and CO2 assimilation in the leaves of transgenic rice plants. In: Plant and Cell Physiology. 2004 ; Vol. 45, No. 5. pp. 521-529.
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