Overexpression of platelet-derived growth factor B and downregulation of PDGF-receptor alpha in human immortalized fibroblasts.

C. Gao, M. Miyazaki, T. Kondo, T. Tsuji, Masakiyo Sakaguchi, M. Namba

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Since immortalization of cells is critical in multistep carcinogenesis, efforts should be made to elucidate the mechanisms of the immortalization. To determine whether platelet-derived growth factor (PDGF) signal pathways play a role in immortalization of cells, we compared mRNA expressions of PDGFs and their receptors in three immortalized human fibroblast cell lines (SUSM-1, OUMS-24F, and KMST-6) with their normal parent cells. As a result, mRNA expression of PDGF-B (oncogene: c-sis) was upregulated in these immortalized cells. Unexpectedly, the expression of alpha- and beta-PDGF receptor genes was downregulated. PDGFR-alpha mRNA was remarkably decreased. When exogenous PDGFR-alpha was expressed transiently in the KMST-6 cells, the morphology of the cells resembled that of normal cells. These results suggest that the overexpression of PDGF-B (c-sis) and downregulation of PDGFR-alpha are related to the phenotypic characteristics of immortalized human cells.

Original languageEnglish
Pages (from-to)871-875
Number of pages5
JournalInternational Journal of Oncology
Volume18
Issue number4
Publication statusPublished - Apr 2001

Fingerprint

Platelet-Derived Growth Factor alpha Receptor
Proto-Oncogene Proteins c-sis
Down-Regulation
Fibroblasts
Messenger RNA
sis Genes
Platelet-Derived Growth Factor beta Receptor
Platelet-Derived Growth Factor Receptors
Platelet-Derived Growth Factor
Signal Transduction
Carcinogenesis
Cell Line

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Overexpression of platelet-derived growth factor B and downregulation of PDGF-receptor alpha in human immortalized fibroblasts. / Gao, C.; Miyazaki, M.; Kondo, T.; Tsuji, T.; Sakaguchi, Masakiyo; Namba, M.

In: International Journal of Oncology, Vol. 18, No. 4, 04.2001, p. 871-875.

Research output: Contribution to journalArticle

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AU - Gao, C.

AU - Miyazaki, M.

AU - Kondo, T.

AU - Tsuji, T.

AU - Sakaguchi, Masakiyo

AU - Namba, M.

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N2 - Since immortalization of cells is critical in multistep carcinogenesis, efforts should be made to elucidate the mechanisms of the immortalization. To determine whether platelet-derived growth factor (PDGF) signal pathways play a role in immortalization of cells, we compared mRNA expressions of PDGFs and their receptors in three immortalized human fibroblast cell lines (SUSM-1, OUMS-24F, and KMST-6) with their normal parent cells. As a result, mRNA expression of PDGF-B (oncogene: c-sis) was upregulated in these immortalized cells. Unexpectedly, the expression of alpha- and beta-PDGF receptor genes was downregulated. PDGFR-alpha mRNA was remarkably decreased. When exogenous PDGFR-alpha was expressed transiently in the KMST-6 cells, the morphology of the cells resembled that of normal cells. These results suggest that the overexpression of PDGF-B (c-sis) and downregulation of PDGFR-alpha are related to the phenotypic characteristics of immortalized human cells.

AB - Since immortalization of cells is critical in multistep carcinogenesis, efforts should be made to elucidate the mechanisms of the immortalization. To determine whether platelet-derived growth factor (PDGF) signal pathways play a role in immortalization of cells, we compared mRNA expressions of PDGFs and their receptors in three immortalized human fibroblast cell lines (SUSM-1, OUMS-24F, and KMST-6) with their normal parent cells. As a result, mRNA expression of PDGF-B (oncogene: c-sis) was upregulated in these immortalized cells. Unexpectedly, the expression of alpha- and beta-PDGF receptor genes was downregulated. PDGFR-alpha mRNA was remarkably decreased. When exogenous PDGFR-alpha was expressed transiently in the KMST-6 cells, the morphology of the cells resembled that of normal cells. These results suggest that the overexpression of PDGF-B (c-sis) and downregulation of PDGFR-alpha are related to the phenotypic characteristics of immortalized human cells.

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