Abstract
The Arabidopsis genome encodes six members of microRNA395 (miR395) family previously determined to regulate the expression of ATP sulfurylase (APS) and the sulfate transporter SULTR2;1. However, the mRNA targets for the individual miR395 family members and the biological consequences produced by target gene regulation of each miR395 remain to be identified. In this study, a transgenic approach was employed to determine the mRNA targets for each miR395 family member as well as the role each member plays in plant growth under abiotic stress conditions. Over-expression of miR395c or miR395e retarded and accelerated, respectively, the seed germination of Arabidopsis under high salt or dehydration stress conditions. Despite a single nucleotide difference between miR395c and miR395e, the cleavage of mRNA targets,APS1,APS3, APS4 and SULTR2;1, was not same in miR395c- and miR395e-overexpressing plants. These results demonstrate that a given miRNA family containing a single nucleotide difference can guide the cleavage of various mRNA targets, thereby acting as a positive or negative regulator of seed germination under stress.
| Original language | English |
|---|---|
| Pages (from-to) | 1447-1454 |
| Number of pages | 8 |
| Journal | Planta |
| Volume | 232 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2010 |
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Keywords
- Abiotic stress
- Arabidopsis
- MicroRNA miR395
- Sulfate transporter
ASJC Scopus subject areas
- Plant Science
- Genetics
Cite this
Overexpression of microRNA395c or 395e affects differently the seed germination of arabidopsis thaliana under stress conditions. / Kim, Joo Yeol; Lee, Hwa Jung; Jung, Hyun Ju; Maruyama, Kazuyuki; Suzuki, Nobuhiro; Kang, Hunseung.
In: Planta, Vol. 232, No. 6, 2010, p. 1447-1454.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Overexpression of microRNA395c or 395e affects differently the seed germination of arabidopsis thaliana under stress conditions
AU - Kim, Joo Yeol
AU - Lee, Hwa Jung
AU - Jung, Hyun Ju
AU - Maruyama, Kazuyuki
AU - Suzuki, Nobuhiro
AU - Kang, Hunseung
PY - 2010
Y1 - 2010
N2 - The Arabidopsis genome encodes six members of microRNA395 (miR395) family previously determined to regulate the expression of ATP sulfurylase (APS) and the sulfate transporter SULTR2;1. However, the mRNA targets for the individual miR395 family members and the biological consequences produced by target gene regulation of each miR395 remain to be identified. In this study, a transgenic approach was employed to determine the mRNA targets for each miR395 family member as well as the role each member plays in plant growth under abiotic stress conditions. Over-expression of miR395c or miR395e retarded and accelerated, respectively, the seed germination of Arabidopsis under high salt or dehydration stress conditions. Despite a single nucleotide difference between miR395c and miR395e, the cleavage of mRNA targets,APS1,APS3, APS4 and SULTR2;1, was not same in miR395c- and miR395e-overexpressing plants. These results demonstrate that a given miRNA family containing a single nucleotide difference can guide the cleavage of various mRNA targets, thereby acting as a positive or negative regulator of seed germination under stress.
AB - The Arabidopsis genome encodes six members of microRNA395 (miR395) family previously determined to regulate the expression of ATP sulfurylase (APS) and the sulfate transporter SULTR2;1. However, the mRNA targets for the individual miR395 family members and the biological consequences produced by target gene regulation of each miR395 remain to be identified. In this study, a transgenic approach was employed to determine the mRNA targets for each miR395 family member as well as the role each member plays in plant growth under abiotic stress conditions. Over-expression of miR395c or miR395e retarded and accelerated, respectively, the seed germination of Arabidopsis under high salt or dehydration stress conditions. Despite a single nucleotide difference between miR395c and miR395e, the cleavage of mRNA targets,APS1,APS3, APS4 and SULTR2;1, was not same in miR395c- and miR395e-overexpressing plants. These results demonstrate that a given miRNA family containing a single nucleotide difference can guide the cleavage of various mRNA targets, thereby acting as a positive or negative regulator of seed germination under stress.
KW - Abiotic stress
KW - Arabidopsis
KW - MicroRNA miR395
KW - Sulfate transporter
UR - http://www.scopus.com/inward/record.url?scp=78650304828&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78650304828&partnerID=8YFLogxK
U2 - 10.1007/s00425-010-1267-x
DO - 10.1007/s00425-010-1267-x
M3 - Article
C2 - 20839006
AN - SCOPUS:78650304828
VL - 232
SP - 1447
EP - 1454
JO - Planta
JF - Planta
SN - 0032-0935
IS - 6
ER -