Overexpression of microRNA395c or 395e affects differently the seed germination of arabidopsis thaliana under stress conditions

Joo Yeol Kim, Hwa Jung Lee, Hyun Ju Jung, Kazuyuki Maruyama, Nobuhiro Suzuki, Hunseung Kang

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The Arabidopsis genome encodes six members of microRNA395 (miR395) family previously determined to regulate the expression of ATP sulfurylase (APS) and the sulfate transporter SULTR2;1. However, the mRNA targets for the individual miR395 family members and the biological consequences produced by target gene regulation of each miR395 remain to be identified. In this study, a transgenic approach was employed to determine the mRNA targets for each miR395 family member as well as the role each member plays in plant growth under abiotic stress conditions. Over-expression of miR395c or miR395e retarded and accelerated, respectively, the seed germination of Arabidopsis under high salt or dehydration stress conditions. Despite a single nucleotide difference between miR395c and miR395e, the cleavage of mRNA targets,APS1,APS3, APS4 and SULTR2;1, was not same in miR395c- and miR395e-overexpressing plants. These results demonstrate that a given miRNA family containing a single nucleotide difference can guide the cleavage of various mRNA targets, thereby acting as a positive or negative regulator of seed germination under stress.

Original languageEnglish
Pages (from-to)1447-1454
Number of pages8
JournalPlanta
Volume232
Issue number6
DOIs
Publication statusPublished - 2010

Fingerprint

Germination
Arabidopsis
Seeds
seed germination
Arabidopsis thaliana
nucleotides
sulfate adenylyltransferase
Messenger RNA
desiccation (plant physiology)
microRNA
abiotic stress
transporters
Sulfate Adenylyltransferase
sulfates
Nucleotides
plant growth
genetically modified organisms
salts
genome
MicroRNAs

Keywords

  • Abiotic stress
  • Arabidopsis
  • MicroRNA miR395
  • Sulfate transporter

ASJC Scopus subject areas

  • Plant Science
  • Genetics

Cite this

Overexpression of microRNA395c or 395e affects differently the seed germination of arabidopsis thaliana under stress conditions. / Kim, Joo Yeol; Lee, Hwa Jung; Jung, Hyun Ju; Maruyama, Kazuyuki; Suzuki, Nobuhiro; Kang, Hunseung.

In: Planta, Vol. 232, No. 6, 2010, p. 1447-1454.

Research output: Contribution to journalArticle

Kim, Joo Yeol ; Lee, Hwa Jung ; Jung, Hyun Ju ; Maruyama, Kazuyuki ; Suzuki, Nobuhiro ; Kang, Hunseung. / Overexpression of microRNA395c or 395e affects differently the seed germination of arabidopsis thaliana under stress conditions. In: Planta. 2010 ; Vol. 232, No. 6. pp. 1447-1454.
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AU - Suzuki, Nobuhiro

AU - Kang, Hunseung

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