Abstract
The Arabidopsis genome encodes six members of microRNA395 (miR395) family previously determined to regulate the expression of ATP sulfurylase (APS) and the sulfate transporter SULTR2;1. However, the mRNA targets for the individual miR395 family members and the biological consequences produced by target gene regulation of each miR395 remain to be identified. In this study, a transgenic approach was employed to determine the mRNA targets for each miR395 family member as well as the role each member plays in plant growth under abiotic stress conditions. Over-expression of miR395c or miR395e retarded and accelerated, respectively, the seed germination of Arabidopsis under high salt or dehydration stress conditions. Despite a single nucleotide difference between miR395c and miR395e, the cleavage of mRNA targets,APS1,APS3, APS4 and SULTR2;1, was not same in miR395c- and miR395e-overexpressing plants. These results demonstrate that a given miRNA family containing a single nucleotide difference can guide the cleavage of various mRNA targets, thereby acting as a positive or negative regulator of seed germination under stress.
Original language | English |
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Pages (from-to) | 1447-1454 |
Number of pages | 8 |
Journal | Planta |
Volume | 232 |
Issue number | 6 |
DOIs | |
Publication status | Published - 2010 |
Keywords
- Abiotic stress
- Arabidopsis
- MicroRNA miR395
- Sulfate transporter
ASJC Scopus subject areas
- Genetics
- Plant Science