Overexpression of manganese superoxide dismutase gene suppresses spontaneous apoptosis without a resultant alteration in in vivo growth of the mouse fibrosarcoma, FSa-II

Masahiro Kuroda; Kengo Himei; Daret K. St. Clair; Muneyasu Urano; Tadashi Yoshino; Tadaatsu Akagi; Junichi Asaumi; Shiro Akaki; Yoshihiro Takeda; Susumu Kanazawa; Yoshio Hiraki

Overexpression of manganese superoxide dismutase gene suppresses spontaneous apoptosis without a resultant alteration in in vivo growth of the mouse fibrosarcoma, FSa-II

Masahiro Kuroda, Kengo Himei, Daret K. St. Clair, Muneyasu Urano, Tadashi Yoshino, Tadaatsu Akagi, Junichi Asaumi, Shiro Akaki, Yoshihiro Takeda, Susumu Kanazawa, Yoshio Hiraki

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

The relationship between spontaneous apoptosis and overexpression of manganese superoxide dismutase (MnSOD) gene was examined in vivo. The mouse fibrosarcoma cells expressing high MnSOD activities due to transfection with the human MnSOD cDNA (SOD-H) or the fibrosarcoma cells transfected with the selectable marker alone (NEO) were transplanted into immune-deficient Fox Chase SCID C.B-17/Icr-scid Jcl mice. Apoptosis in tumors was visually quantified by the in situ end-labeling method. The number of apoptotic cells in the SOD-H tumors was significantly less than that in the NEO tumors. The tumor growth time of the SOD-H tumors to grow from 34 to 500 mm³ in one-half of the mice was slightly longer than that of the NEO tumors, but the difference was not statistically significant. These results suggest that overexpression of MnSOD gene is involved in the suppression of spontaneous apoptosis, without a resultant alteration in the tumor growth.

Original language	English
Pages (from-to)	7-10
Number of pages	4
Journal	Anticancer research
Volume	20
Issue number	1 A
Publication status	Published - Apr 18 2000

Keywords

Apoptosis
Gene transfection
In situ end-labeling
Manganese superoxide dismutase
Tumor growth time

ASJC Scopus subject areas

Oncology
Cancer Research

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Overexpression of manganese superoxide dismutase gene suppresses spontaneous apoptosis without a resultant alteration in in vivo growth of the mouse fibrosarcoma, FSa-II. / Kuroda, Masahiro; Himei, Kengo; St. Clair, Daret K.; Urano, Muneyasu; Yoshino, Tadashi; Akagi, Tadaatsu; Asaumi, Junichi; Akaki, Shiro; Takeda, Yoshihiro ; Kanazawa, Susumu; Hiraki, Yoshio.

In: Anticancer research, Vol. 20, No. 1 A, 18.04.2000, p. 7-10.

Research output: Contribution to journal › Article › peer-review

Kuroda, Masahiro ; Himei, Kengo ; St. Clair, Daret K. ; Urano, Muneyasu ; Yoshino, Tadashi ; Akagi, Tadaatsu ; Asaumi, Junichi ; Akaki, Shiro ; Takeda, Yoshihiro ; Kanazawa, Susumu ; Hiraki, Yoshio. / Overexpression of manganese superoxide dismutase gene suppresses spontaneous apoptosis without a resultant alteration in in vivo growth of the mouse fibrosarcoma, FSa-II. In: Anticancer research. 2000 ; Vol. 20, No. 1 A. pp. 7-10.

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title = "Overexpression of manganese superoxide dismutase gene suppresses spontaneous apoptosis without a resultant alteration in in vivo growth of the mouse fibrosarcoma, FSa-II",

abstract = "The relationship between spontaneous apoptosis and overexpression of manganese superoxide dismutase (MnSOD) gene was examined in vivo. The mouse fibrosarcoma cells expressing high MnSOD activities due to transfection with the human MnSOD cDNA (SOD-H) or the fibrosarcoma cells transfected with the selectable marker alone (NEO) were transplanted into immune-deficient Fox Chase SCID C.B-17/Icr-scid Jcl mice. Apoptosis in tumors was visually quantified by the in situ end-labeling method. The number of apoptotic cells in the SOD-H tumors was significantly less than that in the NEO tumors. The tumor growth time of the SOD-H tumors to grow from 34 to 500 mm3 in one-half of the mice was slightly longer than that of the NEO tumors, but the difference was not statistically significant. These results suggest that overexpression of MnSOD gene is involved in the suppression of spontaneous apoptosis, without a resultant alteration in the tumor growth.",

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author = "Masahiro Kuroda and Kengo Himei and {St. Clair}, {Daret K.} and Muneyasu Urano and Tadashi Yoshino and Tadaatsu Akagi and Junichi Asaumi and Shiro Akaki and Yoshihiro Takeda and Susumu Kanazawa and Yoshio Hiraki",

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AU - Urano, Muneyasu

AU - Yoshino, Tadashi

AU - Akagi, Tadaatsu

AU - Asaumi, Junichi

AU - Akaki, Shiro

AU - Takeda, Yoshihiro

AU - Kanazawa, Susumu

AU - Hiraki, Yoshio

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N2 - The relationship between spontaneous apoptosis and overexpression of manganese superoxide dismutase (MnSOD) gene was examined in vivo. The mouse fibrosarcoma cells expressing high MnSOD activities due to transfection with the human MnSOD cDNA (SOD-H) or the fibrosarcoma cells transfected with the selectable marker alone (NEO) were transplanted into immune-deficient Fox Chase SCID C.B-17/Icr-scid Jcl mice. Apoptosis in tumors was visually quantified by the in situ end-labeling method. The number of apoptotic cells in the SOD-H tumors was significantly less than that in the NEO tumors. The tumor growth time of the SOD-H tumors to grow from 34 to 500 mm3 in one-half of the mice was slightly longer than that of the NEO tumors, but the difference was not statistically significant. These results suggest that overexpression of MnSOD gene is involved in the suppression of spontaneous apoptosis, without a resultant alteration in the tumor growth.

AB - The relationship between spontaneous apoptosis and overexpression of manganese superoxide dismutase (MnSOD) gene was examined in vivo. The mouse fibrosarcoma cells expressing high MnSOD activities due to transfection with the human MnSOD cDNA (SOD-H) or the fibrosarcoma cells transfected with the selectable marker alone (NEO) were transplanted into immune-deficient Fox Chase SCID C.B-17/Icr-scid Jcl mice. Apoptosis in tumors was visually quantified by the in situ end-labeling method. The number of apoptotic cells in the SOD-H tumors was significantly less than that in the NEO tumors. The tumor growth time of the SOD-H tumors to grow from 34 to 500 mm3 in one-half of the mice was slightly longer than that of the NEO tumors, but the difference was not statistically significant. These results suggest that overexpression of MnSOD gene is involved in the suppression of spontaneous apoptosis, without a resultant alteration in the tumor growth.

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