Overexpression of manganese superoxide dismutase gene suppresses spontaneous apoptosis without a resultant alteration in in vivo growth of the mouse fibrosarcoma, FSa-II

Masahiro Kuroda, Kengo Himei, Daret K. St. Clair, Muneyasu Urano, Tadashi Yoshino, Tadaatsu Akagi, Junichi Asaumi, Shiro Akaki, Yoshihiro Takeda, Susumu Kanazawa, Yoshio Hiraki

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Abstract

The relationship between spontaneous apoptosis and overexpression of manganese superoxide dismutase (MnSOD) gene was examined in vivo. The mouse fibrosarcoma cells expressing high MnSOD activities due to transfection with the human MnSOD cDNA (SOD-H) or the fibrosarcoma cells transfected with the selectable marker alone (NEO) were transplanted into immune-deficient Fox Chase SCID C.B-17/Icr-scid Jcl mice. Apoptosis in tumors was visually quantified by the in situ end-labeling method. The number of apoptotic cells in the SOD-H tumors was significantly less than that in the NEO tumors. The tumor growth time of the SOD-H tumors to grow from 34 to 500 mm3 in one-half of the mice was slightly longer than that of the NEO tumors, but the difference was not statistically significant. These results suggest that overexpression of MnSOD gene is involved in the suppression of spontaneous apoptosis, without a resultant alteration in the tumor growth.

Original languageEnglish
Pages (from-to)7-10
Number of pages4
JournalAnticancer research
Volume20
Issue number1 A
Publication statusPublished - Apr 18 2000

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Keywords

  • Apoptosis
  • Gene transfection
  • In situ end-labeling
  • Manganese superoxide dismutase
  • Tumor growth time

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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