Abstract
The relationship between spontaneous apoptosis and overexpression of manganese superoxide dismutase (MnSOD) gene was examined in vivo. The mouse fibrosarcoma cells expressing high MnSOD activities due to transfection with the human MnSOD cDNA (SOD-H) or the fibrosarcoma cells transfected with the selectable marker alone (NEO) were transplanted into immune-deficient Fox Chase SCID C.B-17/Icr-scid Jcl mice. Apoptosis in tumors was visually quantified by the in situ end-labeling method. The number of apoptotic cells in the SOD-H tumors was significantly less than that in the NEO tumors. The tumor growth time of the SOD-H tumors to grow from 34 to 500 mm3 in one-half of the mice was slightly longer than that of the NEO tumors, but the difference was not statistically significant. These results suggest that overexpression of MnSOD gene is involved in the suppression of spontaneous apoptosis, without a resultant alteration in the tumor growth.
| Original language | English |
|---|---|
| Pages (from-to) | 7-10 |
| Number of pages | 4 |
| Journal | Anticancer Research |
| Volume | 20 |
| Issue number | 1 A |
| Publication status | Published - 2000 |
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Keywords
- Apoptosis
- Gene transfection
- In situ end-labeling
- Manganese superoxide dismutase
- Tumor growth time
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Overexpression of manganese superoxide dismutase gene suppresses spontaneous apoptosis without a resultant alteration in in vivo growth of the mouse fibrosarcoma, FSa-II. / Kuroda, Masahiro; Himei, Kengo; St. Clair, Daret K.; Urano, Muneyasu; Yoshino, Tadashi; Akagi, Tadaatsu; Asaumi, Jun-Ichi; Akaki, Shiro; Takeda, Yoshihiro; Kanazawa, Susumu; Hiraki, Yoshio.
In: Anticancer Research, Vol. 20, No. 1 A, 2000, p. 7-10.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Overexpression of manganese superoxide dismutase gene suppresses spontaneous apoptosis without a resultant alteration in in vivo growth of the mouse fibrosarcoma, FSa-II
AU - Kuroda, Masahiro
AU - Himei, Kengo
AU - St. Clair, Daret K.
AU - Urano, Muneyasu
AU - Yoshino, Tadashi
AU - Akagi, Tadaatsu
AU - Asaumi, Jun-Ichi
AU - Akaki, Shiro
AU - Takeda, Yoshihiro
AU - Kanazawa, Susumu
AU - Hiraki, Yoshio
PY - 2000
Y1 - 2000
N2 - The relationship between spontaneous apoptosis and overexpression of manganese superoxide dismutase (MnSOD) gene was examined in vivo. The mouse fibrosarcoma cells expressing high MnSOD activities due to transfection with the human MnSOD cDNA (SOD-H) or the fibrosarcoma cells transfected with the selectable marker alone (NEO) were transplanted into immune-deficient Fox Chase SCID C.B-17/Icr-scid Jcl mice. Apoptosis in tumors was visually quantified by the in situ end-labeling method. The number of apoptotic cells in the SOD-H tumors was significantly less than that in the NEO tumors. The tumor growth time of the SOD-H tumors to grow from 34 to 500 mm3 in one-half of the mice was slightly longer than that of the NEO tumors, but the difference was not statistically significant. These results suggest that overexpression of MnSOD gene is involved in the suppression of spontaneous apoptosis, without a resultant alteration in the tumor growth.
AB - The relationship between spontaneous apoptosis and overexpression of manganese superoxide dismutase (MnSOD) gene was examined in vivo. The mouse fibrosarcoma cells expressing high MnSOD activities due to transfection with the human MnSOD cDNA (SOD-H) or the fibrosarcoma cells transfected with the selectable marker alone (NEO) were transplanted into immune-deficient Fox Chase SCID C.B-17/Icr-scid Jcl mice. Apoptosis in tumors was visually quantified by the in situ end-labeling method. The number of apoptotic cells in the SOD-H tumors was significantly less than that in the NEO tumors. The tumor growth time of the SOD-H tumors to grow from 34 to 500 mm3 in one-half of the mice was slightly longer than that of the NEO tumors, but the difference was not statistically significant. These results suggest that overexpression of MnSOD gene is involved in the suppression of spontaneous apoptosis, without a resultant alteration in the tumor growth.
KW - Apoptosis
KW - Gene transfection
KW - In situ end-labeling
KW - Manganese superoxide dismutase
KW - Tumor growth time
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UR - http://www.scopus.com/inward/citedby.url?scp=0034105109&partnerID=8YFLogxK
M3 - Article
VL - 20
SP - 7
EP - 10
JO - Anticancer Research
JF - Anticancer Research
SN - 0250-7005
IS - 1 A
ER -