Abstract
Aluminum (Al) inhibits plant growth partly by causing oxidative damage that is promoted by reactive oxygen species and can be prevented by improving antioxidant capacity. Ascorbic acid (AsA), the most abundant antioxidant in plants, is regenerated by the action of monodehydroascorbate reductase (MDAR) and dehydroascorbate reductase (DHAR). We investigated the role of MDAR and DHAR in AsA regeneration during Al stress using transgenic tobacco (Nicotiana tabacum) plants overexpressing Arabidopsis cytosolic MDAR (MDAR-OX) or DHAR (DHAR-OX). DHAR-OX plants showed better root growth than wild-type (SR-1) plants after exposure to Al for 2 weeks, but MDAR-OX plants did not. There was no difference in Al distribution and accumulation in the root tips among SR-1, DHAR-OX, and MDAR-OX plants after Al treatment for 24 h. However, DHAR-OX plants showed lower hydrogen peroxide content, less lipid peroxidation and lower level of oxidative DNA damage than SR-1 plants, whereas MDAR-OX plants showed the same extent of damage as SR-1 plants. Compared with SR-1 plants, DHAR-OX plants consistently maintained a higher AsA level both with and without Al exposure, while MDAR-OX plants maintained a higher AsA level only without Al exposure. Also, DHAR-OX plants maintained higher APX activity under Al stress. The higher AsA level and APX activity in DHAR-OX plants contributed to their higher antioxidant capacity and higher tolerance to Al stress. These findings show that the overexpression of DHAR, but not of MDAR, confers Al tolerance, and that maintenance of a high AsA level is important to Al tolerance.
| Original language | English |
|---|---|
| Pages (from-to) | 609-621 |
| Number of pages | 13 |
| Journal | Planta |
| Volume | 231 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jan 2010 |
Fingerprint
Keywords
- Al tolerance
- Aluminum
- Ascorbic acid
- Dehydroascorbate reductase
- Monodehydroascorbate reductase
- Reactive oxygen species
ASJC Scopus subject areas
- Plant Science
- Genetics
Cite this
Overexpression of dehydroascorbate reductase, but not monodehydroascorbate reductase, confers tolerance to Aluminum stress in transgenic Tobacco. / Yin, Lina; Wang, Shiwen; Eltayeb, Amin Elsadig; Uddin, Md Imtiaz; Yamamoto, Yoko; Tsuji, Wataru; Takeuchi, Yuichi; Tanaka, Kiyoshi.
In: Planta, Vol. 231, No. 3, 01.2010, p. 609-621.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Overexpression of dehydroascorbate reductase, but not monodehydroascorbate reductase, confers tolerance to Aluminum stress in transgenic Tobacco
AU - Yin, Lina
AU - Wang, Shiwen
AU - Eltayeb, Amin Elsadig
AU - Uddin, Md Imtiaz
AU - Yamamoto, Yoko
AU - Tsuji, Wataru
AU - Takeuchi, Yuichi
AU - Tanaka, Kiyoshi
PY - 2010/1
Y1 - 2010/1
N2 - Aluminum (Al) inhibits plant growth partly by causing oxidative damage that is promoted by reactive oxygen species and can be prevented by improving antioxidant capacity. Ascorbic acid (AsA), the most abundant antioxidant in plants, is regenerated by the action of monodehydroascorbate reductase (MDAR) and dehydroascorbate reductase (DHAR). We investigated the role of MDAR and DHAR in AsA regeneration during Al stress using transgenic tobacco (Nicotiana tabacum) plants overexpressing Arabidopsis cytosolic MDAR (MDAR-OX) or DHAR (DHAR-OX). DHAR-OX plants showed better root growth than wild-type (SR-1) plants after exposure to Al for 2 weeks, but MDAR-OX plants did not. There was no difference in Al distribution and accumulation in the root tips among SR-1, DHAR-OX, and MDAR-OX plants after Al treatment for 24 h. However, DHAR-OX plants showed lower hydrogen peroxide content, less lipid peroxidation and lower level of oxidative DNA damage than SR-1 plants, whereas MDAR-OX plants showed the same extent of damage as SR-1 plants. Compared with SR-1 plants, DHAR-OX plants consistently maintained a higher AsA level both with and without Al exposure, while MDAR-OX plants maintained a higher AsA level only without Al exposure. Also, DHAR-OX plants maintained higher APX activity under Al stress. The higher AsA level and APX activity in DHAR-OX plants contributed to their higher antioxidant capacity and higher tolerance to Al stress. These findings show that the overexpression of DHAR, but not of MDAR, confers Al tolerance, and that maintenance of a high AsA level is important to Al tolerance.
AB - Aluminum (Al) inhibits plant growth partly by causing oxidative damage that is promoted by reactive oxygen species and can be prevented by improving antioxidant capacity. Ascorbic acid (AsA), the most abundant antioxidant in plants, is regenerated by the action of monodehydroascorbate reductase (MDAR) and dehydroascorbate reductase (DHAR). We investigated the role of MDAR and DHAR in AsA regeneration during Al stress using transgenic tobacco (Nicotiana tabacum) plants overexpressing Arabidopsis cytosolic MDAR (MDAR-OX) or DHAR (DHAR-OX). DHAR-OX plants showed better root growth than wild-type (SR-1) plants after exposure to Al for 2 weeks, but MDAR-OX plants did not. There was no difference in Al distribution and accumulation in the root tips among SR-1, DHAR-OX, and MDAR-OX plants after Al treatment for 24 h. However, DHAR-OX plants showed lower hydrogen peroxide content, less lipid peroxidation and lower level of oxidative DNA damage than SR-1 plants, whereas MDAR-OX plants showed the same extent of damage as SR-1 plants. Compared with SR-1 plants, DHAR-OX plants consistently maintained a higher AsA level both with and without Al exposure, while MDAR-OX plants maintained a higher AsA level only without Al exposure. Also, DHAR-OX plants maintained higher APX activity under Al stress. The higher AsA level and APX activity in DHAR-OX plants contributed to their higher antioxidant capacity and higher tolerance to Al stress. These findings show that the overexpression of DHAR, but not of MDAR, confers Al tolerance, and that maintenance of a high AsA level is important to Al tolerance.
KW - Al tolerance
KW - Aluminum
KW - Ascorbic acid
KW - Dehydroascorbate reductase
KW - Monodehydroascorbate reductase
KW - Reactive oxygen species
UR - http://www.scopus.com/inward/record.url?scp=76049130837&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=76049130837&partnerID=8YFLogxK
U2 - 10.1007/s00425-009-1075-3
DO - 10.1007/s00425-009-1075-3
M3 - Article
C2 - 19960204
AN - SCOPUS:76049130837
VL - 231
SP - 609
EP - 621
JO - Planta
JF - Planta
SN - 0032-0935
IS - 3
ER -