Overexpression of cyclin E in acute myelogenous leukemia

Hiroatsu Lida, Masayuki Towatari, Mitsune Tanimoto, Yoshihisa Morishita, Yoshihisa Kodera, Hidehiko Saito

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Abstract

Cyclin E is one of the G1 cyclins that play an important role in cell proliferation. Overexpression of cyclin E protein has been reported in several solid tumors, but little is known about the involvement of cyclin E in leukemia. In this study, we analyzed the expression of cyclin E gene product in 85 patients with acute myelogenous leukemia (AML) by Western blot analysis. In 23 of 85 AML samples (27%), cyclin E expression was enhanced in blasts. Among the French-American-British classification of AML, the ratio of the samples with enhanced cyclin E expression was high in MS and low in M2 and M3. No rearrangements were observed by Southern blot analysis in these AML blasts with enhanced cyclin E expression. Flow cytometric analysis showed no correlation between overexpression of cyclin E and cell cycle distribution. Immunoblot analysis of cyclin D1 showed no correlation between overexpression of cyclin E and that of cyclin D1. Interestingly, p27 expression detected by Western blotting was apparently enhanced in 18 of 23 AML cells with enhanced cyclin E expression but none of 14 AML cells without enhanced cyclin E exhibited enhanced p27 expression. The rates of complete remission and of disease-free survival of the patients with M4 or M5 leukemia blasts with overexpressed cyclin E seemed to be low. Therefore, we suggest the necessity of a larger-scale study to elucidate the contribution of cyclin E overexpression to the phenotype and the prognosis of certain AML.

Original languageEnglish
Pages (from-to)3707-3713
Number of pages7
JournalBlood
Volume90
Issue number9
DOIs
Publication statusPublished - 1997

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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    Lida, H., Towatari, M., Tanimoto, M., Morishita, Y., Kodera, Y., & Saito, H. (1997). Overexpression of cyclin E in acute myelogenous leukemia. Blood, 90(9), 3707-3713. https://doi.org/10.1182/blood.v90.9.3707