Overexpression of cyclin E in acute myelogenous leukemia

Hiroatsu Lida, Masayuki Towatari, Mitsune Tanimoto, Yoshihisa Morishita, Yoshihisa Kodera, Hidehiko Saito

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Cyclin E is one of the G1 cyclins that play an important role in cell proliferation. Overexpression of cyclin E protein has been reported in several solid tumors, but little is known about the involvement of cyclin E in leukemia. In this study, we analyzed the expression of cyclin E gene product in 85 patients with acute myelogenous leukemia (AML) by Western blot analysis. In 23 of 85 AML samples (27%), cyclin E expression was enhanced in blasts. Among the French-American-British classification of AML, the ratio of the samples with enhanced cyclin E expression was high in MS and low in M2 and M3. No rearrangements were observed by Southern blot analysis in these AML blasts with enhanced cyclin E expression. Flow cytometric analysis showed no correlation between overexpression of cyclin E and cell cycle distribution. Immunoblot analysis of cyclin D1 showed no correlation between overexpression of cyclin E and that of cyclin D1. Interestingly, p27 expression detected by Western blotting was apparently enhanced in 18 of 23 AML cells with enhanced cyclin E expression but none of 14 AML cells without enhanced cyclin E exhibited enhanced p27 expression. The rates of complete remission and of disease-free survival of the patients with M4 or M5 leukemia blasts with overexpressed cyclin E seemed to be low. Therefore, we suggest the necessity of a larger-scale study to elucidate the contribution of cyclin E overexpression to the phenotype and the prognosis of certain AML.

Original languageEnglish
Pages (from-to)3707-3713
Number of pages7
JournalBlood
Volume90
Issue number9
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Cyclin E
Acute Myeloid Leukemia
Cyclin D1
Leukemia
Western Blotting
Cyclin G1
Cell proliferation
Southern Blotting
Disease-Free Survival
Tumors
Cell Cycle

ASJC Scopus subject areas

  • Hematology

Cite this

Lida, H., Towatari, M., Tanimoto, M., Morishita, Y., Kodera, Y., & Saito, H. (1997). Overexpression of cyclin E in acute myelogenous leukemia. Blood, 90(9), 3707-3713.

Overexpression of cyclin E in acute myelogenous leukemia. / Lida, Hiroatsu; Towatari, Masayuki; Tanimoto, Mitsune; Morishita, Yoshihisa; Kodera, Yoshihisa; Saito, Hidehiko.

In: Blood, Vol. 90, No. 9, 1997, p. 3707-3713.

Research output: Contribution to journalArticle

Lida, H, Towatari, M, Tanimoto, M, Morishita, Y, Kodera, Y & Saito, H 1997, 'Overexpression of cyclin E in acute myelogenous leukemia', Blood, vol. 90, no. 9, pp. 3707-3713.
Lida H, Towatari M, Tanimoto M, Morishita Y, Kodera Y, Saito H. Overexpression of cyclin E in acute myelogenous leukemia. Blood. 1997;90(9):3707-3713.
Lida, Hiroatsu ; Towatari, Masayuki ; Tanimoto, Mitsune ; Morishita, Yoshihisa ; Kodera, Yoshihisa ; Saito, Hidehiko. / Overexpression of cyclin E in acute myelogenous leukemia. In: Blood. 1997 ; Vol. 90, No. 9. pp. 3707-3713.
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