Overexpression of c-erbB-3 in various stages of human squamous cell carcinomas

Takaaki Funayama, Tohru Nakanishi, Korjiro Takahashi, Shigehiko Taniguchi, Masaharu Takigawa, Tomohiro Matsumura

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

Amplification of the c-erbB-3 gene and the expression of its mRNA and protein in human squamous cell carcinomas (SCC) were analyzed. Although no amplification of the c-erbB-3 gene was observed, overexpression of its mRNA was observed in SCC by RT-PCR. The expression of this gene in SCC was 15-50 times higher than in normal fibroblasts. Overexpression of the secreted type of erbB-3 mRNA was also observed in all SCC. Moreover, overproduction of the c-erbB-3 protein was also detected in SCC by dot blot analysis using anti-c-erbB-3 monoclonal antibodies. In nude mice, the expression of c-erbB-3 mRNA was higher in metastatic tumors compared with primary tumors. These results suggest that both the transmembrane and secreted types of c-erbB-3 play a significant role in the formation and development of SCC.

Original languageEnglish
Pages (from-to)161-167
Number of pages7
JournalOncology
Volume55
Issue number2
DOIs
Publication statusPublished - 1998

Keywords

  • Immunohistochemical study
  • Oral region
  • Polymerase chain reaction
  • erbB-3 squamous cell carcinoma

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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