Overexpression of c-erbB-3 in various stages of human squamous cell carcinomas

Takaaki Funayama; Tohru Nakanishi; Korjiro Takahashi; Shigehiko Taniguchi; Masaharu Takigawa; Tomohiro Matsumura

doi:10.1159/000011851

Overexpression of c-erbB-3 in various stages of human squamous cell carcinomas

Takaaki Funayama, Tohru Nakanishi, Korjiro Takahashi, Shigehiko Taniguchi, Masaharu Takigawa, Tomohiro Matsumura

Dental School

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

Amplification of the c-erbB-3 gene and the expression of its mRNA and protein in human squamous cell carcinomas (SCC) were analyzed. Although no amplification of the c-erbB-3 gene was observed, overexpression of its mRNA was observed in SCC by RT-PCR. The expression of this gene in SCC was 15-50 times higher than in normal fibroblasts. Overexpression of the secreted type of erbB-3 mRNA was also observed in all SCC. Moreover, overproduction of the c-erbB-3 protein was also detected in SCC by dot blot analysis using anti-c-erbB-3 monoclonal antibodies. In nude mice, the expression of c-erbB-3 mRNA was higher in metastatic tumors compared with primary tumors. These results suggest that both the transmembrane and secreted types of c-erbB-3 play a significant role in the formation and development of SCC.

Original language	English
Pages (from-to)	161-167
Number of pages	7
Journal	Oncology
Volume	55
Issue number	2
DOIs	https://doi.org/10.1159/000011851
Publication status	Published - 1998

Keywords

Immunohistochemical study
Oral region
Polymerase chain reaction
erbB-3 squamous cell carcinoma

ASJC Scopus subject areas

Oncology
Cancer Research

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title = "Overexpression of c-erbB-3 in various stages of human squamous cell carcinomas",

abstract = "Amplification of the c-erbB-3 gene and the expression of its mRNA and protein in human squamous cell carcinomas (SCC) were analyzed. Although no amplification of the c-erbB-3 gene was observed, overexpression of its mRNA was observed in SCC by RT-PCR. The expression of this gene in SCC was 15-50 times higher than in normal fibroblasts. Overexpression of the secreted type of erbB-3 mRNA was also observed in all SCC. Moreover, overproduction of the c-erbB-3 protein was also detected in SCC by dot blot analysis using anti-c-erbB-3 monoclonal antibodies. In nude mice, the expression of c-erbB-3 mRNA was higher in metastatic tumors compared with primary tumors. These results suggest that both the transmembrane and secreted types of c-erbB-3 play a significant role in the formation and development of SCC.",

keywords = "Immunohistochemical study, Oral region, Polymerase chain reaction, erbB-3 squamous cell carcinoma",

author = "Takaaki Funayama and Tohru Nakanishi and Korjiro Takahashi and Shigehiko Taniguchi and Masaharu Takigawa and Tomohiro Matsumura",

year = "1998",

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T1 - Overexpression of c-erbB-3 in various stages of human squamous cell carcinomas

AU - Funayama, Takaaki

AU - Nakanishi, Tohru

AU - Takahashi, Korjiro

AU - Taniguchi, Shigehiko

AU - Takigawa, Masaharu

AU - Matsumura, Tomohiro

PY - 1998

Y1 - 1998

N2 - Amplification of the c-erbB-3 gene and the expression of its mRNA and protein in human squamous cell carcinomas (SCC) were analyzed. Although no amplification of the c-erbB-3 gene was observed, overexpression of its mRNA was observed in SCC by RT-PCR. The expression of this gene in SCC was 15-50 times higher than in normal fibroblasts. Overexpression of the secreted type of erbB-3 mRNA was also observed in all SCC. Moreover, overproduction of the c-erbB-3 protein was also detected in SCC by dot blot analysis using anti-c-erbB-3 monoclonal antibodies. In nude mice, the expression of c-erbB-3 mRNA was higher in metastatic tumors compared with primary tumors. These results suggest that both the transmembrane and secreted types of c-erbB-3 play a significant role in the formation and development of SCC.

AB - Amplification of the c-erbB-3 gene and the expression of its mRNA and protein in human squamous cell carcinomas (SCC) were analyzed. Although no amplification of the c-erbB-3 gene was observed, overexpression of its mRNA was observed in SCC by RT-PCR. The expression of this gene in SCC was 15-50 times higher than in normal fibroblasts. Overexpression of the secreted type of erbB-3 mRNA was also observed in all SCC. Moreover, overproduction of the c-erbB-3 protein was also detected in SCC by dot blot analysis using anti-c-erbB-3 monoclonal antibodies. In nude mice, the expression of c-erbB-3 mRNA was higher in metastatic tumors compared with primary tumors. These results suggest that both the transmembrane and secreted types of c-erbB-3 play a significant role in the formation and development of SCC.

KW - Immunohistochemical study

KW - Oral region

KW - Polymerase chain reaction

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