Abstract
Amplification of the c-erbB-3 gene and the expression of its mRNA and protein in human squamous cell carcinomas (SCC) were analyzed. Although no amplification of the c-erbB-3 gene was observed, overexpression of its mRNA was observed in SCC by RT-PCR. The expression of this gene in SCC was 15-50 times higher than in normal fibroblasts. Overexpression of the secreted type of erbB-3 mRNA was also observed in all SCC. Moreover, overproduction of the c-erbB-3 protein was also detected in SCC by dot blot analysis using anti-c-erbB-3 monoclonal antibodies. In nude mice, the expression of c-erbB-3 mRNA was higher in metastatic tumors compared with primary tumors. These results suggest that both the transmembrane and secreted types of c-erbB-3 play a significant role in the formation and development of SCC.
Original language | English |
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Pages (from-to) | 161-167 |
Number of pages | 7 |
Journal | Oncology |
Volume | 55 |
Issue number | 2 |
DOIs | |
Publication status | Published - 1998 |
Keywords
- Immunohistochemical study
- Oral region
- Polymerase chain reaction
- erbB-3 squamous cell carcinoma
ASJC Scopus subject areas
- Oncology
- Cancer Research