Overexpression of an auxilin-like gene (F9E10.5) can suppress Al uptake in roots of Arabidopsis

Bunichi Ezaki, Hiroyuki Kiyohara, Hideaki Matsumoto, Susumu Nakashima

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Plants resistant to aluminium (Al) stress were isolated from Arabidopsis thaliana enhancer-tagged mutant lines. Compared with the parental Col-7 control line, one of the resistant candidates, #355-2, showed a higher expression of the F9E10.5 gene (At1g75100) on chromosome 1, a lower Al content in whole roots, and a shorter root hair length (∼30%). Both Al influx and associated oxidative stress occurred in root hairs, as well as in root tips of Col-7; however, they were seen only in root tips of #355-2. Transgenic plants overexpressing the F9E10.5 gene showed a slightly higher Al resistance than their parental control line (Ler). The F9E10.5 gene encodes an auxilin-like protein related to the clathrin-uncoating process in endocytosis. Microscopic observation indicated that both Al ion influx and endocytosis activity were lower in root hair cells of the #355-2 line than in those of Col-7. These results suggested that overexpression of this auxilin-like protein inhibits endocytosis in root hair cells by a disturbance of the transport system as in animal cells shown previously. It was also suggested that a part of the Al influx occurred via endocytosis in root hair cells in Arabidopsis. The Al resistance in the #355-2 line may therefore be due to a lower Al uptake via endocytosis in the root hair region.

Original languageEnglish
Pages (from-to)497-506
Number of pages10
JournalJournal of Experimental Botany
Volume58
Issue number3
DOIs
Publication statusPublished - Feb 2007

Fingerprint

Auxilins
Aluminum
Arabidopsis
aluminum
root hairs
endocytosis
uptake mechanisms
Endocytosis
Genes
genes
Meristem
root tips
cells
clathrin
Clathrin
Chromosomes, Human, Pair 1
Genetically Modified Plants
transgenic plants
Proteins
Oxidative Stress

Keywords

  • Activation-tagged mutant line
  • Al influx
  • Aluminium (Al) resistance mechanism
  • Arabidopsis thaliana
  • Auxilin-like protein
  • Endocytosis
  • Oxidative stress
  • Root hair

ASJC Scopus subject areas

  • Plant Science

Cite this

Overexpression of an auxilin-like gene (F9E10.5) can suppress Al uptake in roots of Arabidopsis. / Ezaki, Bunichi; Kiyohara, Hiroyuki; Matsumoto, Hideaki; Nakashima, Susumu.

In: Journal of Experimental Botany, Vol. 58, No. 3, 02.2007, p. 497-506.

Research output: Contribution to journalArticle

Ezaki, Bunichi ; Kiyohara, Hiroyuki ; Matsumoto, Hideaki ; Nakashima, Susumu. / Overexpression of an auxilin-like gene (F9E10.5) can suppress Al uptake in roots of Arabidopsis. In: Journal of Experimental Botany. 2007 ; Vol. 58, No. 3. pp. 497-506.
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