Overexpression of alternative oxidase gene confers aluminum tolerance by altering the respiratory capacity and the response to oxidative stress in tobacco cells

Sanjib Kumar Panda, Lingaraj Sahoo, Maki Katsuhara, Hideaki Matsumoto

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Aluminum (Al) stress represses mitochondrial respiration and produces reactive oxygen species (ROS) in plants. Mitochondrial alternative oxidase (AOX) uncouples respiration from mitochondrial ATP production and may improve plant performance under Al stress by preventing excess accumulation of ROS. We tested respiratory changes and ROS production in isolated mitochondria and whole cell of tobacco (SL, ALT 301) under Al stress. Higher capacities of AOX pathways relative to cytochrome pathways were observed in both isolated mitochondria and whole cells of ALT301 under Al stress. AOX1 when studied showed higher AOX1 expression in ALT 301 than SL cells under stress. In order to study the function of tobacco AOX gene under Al stress, we produced transformed tobacco cell lines by introducing NtAOX1 expressed under the control of the cauliflower mosaic virus (CaMV) 35 S promoter in sensitive (SL) Nicotiana tabacum L. cell lines. The enhancement of endogenous AOX1 expression and AOX protein with or without Al stress was in the order of transformed tobacco cell lines > ALT301 > wild type (SL). A decreased respiratory inhibition and reduced ROS production with a better growth capability were the significant features that characterized AOX1 transformed cell lines under Al stress. These results demonstrated that AOX plays a critical role in Al stress tolerance with an enhanced respiratory capacity, reducing mitochondrial oxidative stress burden and improving the growth capability in tobacco cells.

Original languageEnglish
Pages (from-to)551-563
Number of pages13
JournalMolecular Biotechnology
Volume54
Issue number2
DOIs
Publication statusPublished - Jun 2013

Fingerprint

Oxidative stress
Tobacco
Aluminum
Oxidative Stress
Genes
Transformed Cell Line
Reactive Oxygen Species
Cells
Mitochondria
Oxygen
Respiration
Caulimovirus
Proteins
alternative oxidase
Oxidoreductases
Cytochromes
Growth
Adenosinetriphosphate
Viruses
Adenosine Triphosphate

Keywords

  • Aluminum
  • AOX
  • Mitochondria
  • Tobacco

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Molecular Biology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Overexpression of alternative oxidase gene confers aluminum tolerance by altering the respiratory capacity and the response to oxidative stress in tobacco cells. / Panda, Sanjib Kumar; Sahoo, Lingaraj; Katsuhara, Maki; Matsumoto, Hideaki.

In: Molecular Biotechnology, Vol. 54, No. 2, 06.2013, p. 551-563.

Research output: Contribution to journalArticle

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