Abstract
In previous studies, we demonstrated an over-expression of the dominant-negative isoform of the transcription factor Ikaros, Ik-6, in patients with B-cell malignancies, including blast crisis of chronic myelogenous leukaemia and acute lymphoblastic leukaemia. To investigate the consequence of over-expression of Ik-6 in B cells, we constructed Ik-6 transfectants of the FDH-1 and Ramos cell lines. FDH-1, which was established from a patient with early pre-B acute lymphoblastic leukaemia, undergoes apoptosis with dexamethasone treatment, whereas Ramos undergoes apoptosis following anti-IgM antibody treatment. Compared with the wild type, the over-expression of Ik-6 rendered the FDH-1 and Ramos transfectants resistant to dexamethasone-induced and anti-IgM-induced apoptosis respectively. An immunoblotting study demonstrated bcl-2 upregulation in anti-IgM-induced Ramos Ik-6 transfectants, but not in FDH-1 Ik-6 transfectants. Further investigations of the mechanism of leukaemogenesis associated with the over-expression of Ik-6 are warranted.
| Original language | English |
|---|---|
| Pages (from-to) | 165-169 |
| Number of pages | 5 |
| Journal | British Journal of Haematology |
| Volume | 121 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Apr 2003 |
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Keywords
- Anti-IgM
- Apoptosis
- Dexamethasone
- Dominant-negative isoform
- Ikaros
ASJC Scopus subject areas
- Hematology
Cite this
Over-expression of the dominant-negative isoform of Ikaros confers resistance to dexamethasone-induced and anti-IgM-induced apoptosis. / Sezaki, Nobuo; Ishimaru, Fumihiko; Takata, Minoru; Tabayashi, Takayuki; Nakase, Koichi; Kozuka, Teruhiko; Fujii, Keiko; Nakayama, Hiroyuki; Teshima, Takanori; Harada, Mine; Tanimoto, Mitsune.
In: British Journal of Haematology, Vol. 121, No. 1, 04.2003, p. 165-169.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Over-expression of the dominant-negative isoform of Ikaros confers resistance to dexamethasone-induced and anti-IgM-induced apoptosis
AU - Sezaki, Nobuo
AU - Ishimaru, Fumihiko
AU - Takata, Minoru
AU - Tabayashi, Takayuki
AU - Nakase, Koichi
AU - Kozuka, Teruhiko
AU - Fujii, Keiko
AU - Nakayama, Hiroyuki
AU - Teshima, Takanori
AU - Harada, Mine
AU - Tanimoto, Mitsune
PY - 2003/4
Y1 - 2003/4
N2 - In previous studies, we demonstrated an over-expression of the dominant-negative isoform of the transcription factor Ikaros, Ik-6, in patients with B-cell malignancies, including blast crisis of chronic myelogenous leukaemia and acute lymphoblastic leukaemia. To investigate the consequence of over-expression of Ik-6 in B cells, we constructed Ik-6 transfectants of the FDH-1 and Ramos cell lines. FDH-1, which was established from a patient with early pre-B acute lymphoblastic leukaemia, undergoes apoptosis with dexamethasone treatment, whereas Ramos undergoes apoptosis following anti-IgM antibody treatment. Compared with the wild type, the over-expression of Ik-6 rendered the FDH-1 and Ramos transfectants resistant to dexamethasone-induced and anti-IgM-induced apoptosis respectively. An immunoblotting study demonstrated bcl-2 upregulation in anti-IgM-induced Ramos Ik-6 transfectants, but not in FDH-1 Ik-6 transfectants. Further investigations of the mechanism of leukaemogenesis associated with the over-expression of Ik-6 are warranted.
AB - In previous studies, we demonstrated an over-expression of the dominant-negative isoform of the transcription factor Ikaros, Ik-6, in patients with B-cell malignancies, including blast crisis of chronic myelogenous leukaemia and acute lymphoblastic leukaemia. To investigate the consequence of over-expression of Ik-6 in B cells, we constructed Ik-6 transfectants of the FDH-1 and Ramos cell lines. FDH-1, which was established from a patient with early pre-B acute lymphoblastic leukaemia, undergoes apoptosis with dexamethasone treatment, whereas Ramos undergoes apoptosis following anti-IgM antibody treatment. Compared with the wild type, the over-expression of Ik-6 rendered the FDH-1 and Ramos transfectants resistant to dexamethasone-induced and anti-IgM-induced apoptosis respectively. An immunoblotting study demonstrated bcl-2 upregulation in anti-IgM-induced Ramos Ik-6 transfectants, but not in FDH-1 Ik-6 transfectants. Further investigations of the mechanism of leukaemogenesis associated with the over-expression of Ik-6 are warranted.
KW - Anti-IgM
KW - Apoptosis
KW - Dexamethasone
KW - Dominant-negative isoform
KW - Ikaros
UR - http://www.scopus.com/inward/record.url?scp=0037392235&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037392235&partnerID=8YFLogxK
U2 - 10.1046/j.1365-2141.2003.04263.x
DO - 10.1046/j.1365-2141.2003.04263.x
M3 - Article
C2 - 12670348
AN - SCOPUS:0037392235
VL - 121
SP - 165
EP - 169
JO - British Journal of Haematology
JF - British Journal of Haematology
SN - 0007-1048
IS - 1
ER -