Over-expression of the dominant-negative isoform of Ikaros confers resistance to dexamethasone-induced and anti-IgM-induced apoptosis

Nobuo Sezaki, Fumihiko Ishimaru, Minoru Takata, Takayuki Tabayashi, Koichi Nakase, Teruhiko Kozuka, Keiko Fujii, Hiroyuki Nakayama, Takanori Teshima, Mine Harada, Mitsune Tanimoto

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11 Citations (Scopus)

Abstract

In previous studies, we demonstrated an over-expression of the dominant-negative isoform of the transcription factor Ikaros, Ik-6, in patients with B-cell malignancies, including blast crisis of chronic myelogenous leukaemia and acute lymphoblastic leukaemia. To investigate the consequence of over-expression of Ik-6 in B cells, we constructed Ik-6 transfectants of the FDH-1 and Ramos cell lines. FDH-1, which was established from a patient with early pre-B acute lymphoblastic leukaemia, undergoes apoptosis with dexamethasone treatment, whereas Ramos undergoes apoptosis following anti-IgM antibody treatment. Compared with the wild type, the over-expression of Ik-6 rendered the FDH-1 and Ramos transfectants resistant to dexamethasone-induced and anti-IgM-induced apoptosis respectively. An immunoblotting study demonstrated bcl-2 upregulation in anti-IgM-induced Ramos Ik-6 transfectants, but not in FDH-1 Ik-6 transfectants. Further investigations of the mechanism of leukaemogenesis associated with the over-expression of Ik-6 are warranted.

Original languageEnglish
Pages (from-to)165-169
Number of pages5
JournalBritish Journal of Haematology
Volume121
Issue number1
DOIs
Publication statusPublished - Apr 2003

Keywords

  • Anti-IgM
  • Apoptosis
  • Dexamethasone
  • Dominant-negative isoform
  • Ikaros

ASJC Scopus subject areas

  • Hematology

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    Sezaki, N., Ishimaru, F., Takata, M., Tabayashi, T., Nakase, K., Kozuka, T., Fujii, K., Nakayama, H., Teshima, T., Harada, M., & Tanimoto, M. (2003). Over-expression of the dominant-negative isoform of Ikaros confers resistance to dexamethasone-induced and anti-IgM-induced apoptosis. British Journal of Haematology, 121(1), 165-169. https://doi.org/10.1046/j.1365-2141.2003.04263.x