Over-expression of a Barley Aquaporin Increased the Shoot/Root Ratio and Raised Salt Sensitivity in Transgenic Rice Plants

Maki Katsuhara, Kazuki Koshio, Mineo Shibasaka, Yasuyuki Hayashi, Takahiko Hayakawa, Kunihiro Kasamo

Research output: Contribution to journalArticle

126 Citations (Scopus)

Abstract

Barley HvPIP2;1 is a plasma membrane aquaporin and its expression was down-regulated after salt stress in barley [Katsuhara et al. (2002) Plant Cell Physiol. 43: 885]. We produced and analyzed transgenic rice plants over-expressing barley HvPIP2;1 in the present study. Over-expression of HvPIP2;1 increased (1) radial hydraulic conductivity of roots (Lpr) to 140%, and (2) the mass ratio of shoot to root up to 150%. In these transgenic rice plants under salt stress of 100 mM NaCl, growth reduction was greater than in non-transgenic plants. A decrease in shoot water content (from 79% to 61%) and reduction of root mass or shoot mass (both less than 40% of non-stressed plants) were observed in transgenic plants under salt stress for 2 weeks. These results indicated that over-expression of HvPIP2;1 makes rice plants sensitive to 100 mM NaCl. The possible involvement of aquaporins in salt tolerance is discussed.

Original languageEnglish
Pages (from-to)1378-1383
Number of pages6
JournalPlant and Cell Physiology
Volume44
Issue number12
DOIs
Publication statusPublished - Dec 2003

Fingerprint

Aquaporins
aquaporins
Genetically Modified Plants
Hordeum
root shoot ratio
Salts
barley
genetically modified organisms
salts
rice
Salt-Tolerance
salt stress
Plant Cells
Cell Membrane
root hydraulic conductivity
shoots
Water
Growth
salt tolerance
growth retardation

Keywords

  • Aquaporin
  • Barley
  • Salt stress
  • Transgenic rice
  • Water transport

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Over-expression of a Barley Aquaporin Increased the Shoot/Root Ratio and Raised Salt Sensitivity in Transgenic Rice Plants. / Katsuhara, Maki; Koshio, Kazuki; Shibasaka, Mineo; Hayashi, Yasuyuki; Hayakawa, Takahiko; Kasamo, Kunihiro.

In: Plant and Cell Physiology, Vol. 44, No. 12, 12.2003, p. 1378-1383.

Research output: Contribution to journalArticle

Katsuhara, Maki ; Koshio, Kazuki ; Shibasaka, Mineo ; Hayashi, Yasuyuki ; Hayakawa, Takahiko ; Kasamo, Kunihiro. / Over-expression of a Barley Aquaporin Increased the Shoot/Root Ratio and Raised Salt Sensitivity in Transgenic Rice Plants. In: Plant and Cell Physiology. 2003 ; Vol. 44, No. 12. pp. 1378-1383.
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