Ovarian steroids regulate prostaglandin secretion in the feline endometrium

Marta J. Siemieniuch, Anom Bowolaksono, Dariusz J. Skarzynski, Kiyoshi Okuda

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Sex steroids, i.e. progesterone (P4) and 17β-estradiol (E2), fluctuate during the feline estrous cycle and their alterations correspond to many events in cat reproduction. In order to investigate possible effects of sex steroids on prostaglandin (PG) secretion in the cultured endometrial cells, mRNA expressions coding for PG-endoperoxide synthase (PTGS2) in the epithelial and stromal cells harvested with sex steroids were studied by RT-PCR. The effects of ovarian steroids on PG secretion in the epithelial and stromal cells were also investigated. E2 at a dose 10-7M significantly increased prostaglandin F2α (PGF) secretion in the epithelial cells (P<0.01). PGF production was intensified by the treatment in combination with both steroids (P<0.001). P4 at any dose alone had no effect on PGF secretion in the epithelial cells, whereas at a dose 10-5M enhanced prostaglandin E2 (PGE2) production (P<0.05). The ovarian steroids stimulated both PGF and PGE2 in the epithelial cells of the feline endometrium via an E2 receptor (ESR1)- and P4 receptor (PGR)-dependent genomic-pathway. In contrast to the epithelial cells, neither P4 nor E2 affected PG secretion in the stromal cells. PTGS2 mRNA expression was not affected by ovarian steroids in either cell types. The overall results suggest that PG secretion is regulated by P4 and E2 and this effect is not due to changes in PTGS2 mRNA expression.

Original languageEnglish
Pages (from-to)142-150
Number of pages9
JournalAnimal Reproduction Science
Volume120
Issue number1-4
DOIs
Publication statusPublished - Jul 1 2010

Keywords

  • Endometrium
  • Felines
  • Prostaglandin

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology
  • Endocrinology

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