Abstract
Ovarian steroids assure an optimum environment for the final maturation of oocytes, gamete transport, fertilization, and early embryonic development. The aim of experiment 1 was to examine the influence of ovarian steroids on tumor necrosis factor-α (TNF-α) or nitric oxide (NO)regulated prostaglandin (PG), and nitrite/nitrate (NO2/NO3) secretion by cultured bovine oviductal epithelial cells (BOECs). BOECs were pretreated with 17β-estradiol (E2; 109 M) and/or progesterone (P4; 107 M) for 24 h. For the next 24 h, BOECs were treated with TNF-α (10 ng/mL) or spermine nitric oxide complex (NONOate; 105 M). Prostaglandin F2α and PGE2 secretion was measured in medium by ELISA. The pretreatment of cells with P4 (progesterone), E2 (17 β-estradiol), or E2/P4 augmented TNF-αinduced PGF2α and PGE2 secretion (P <0.01). The pretreatment of cells with E2 or E2/P4 increased NONOate-induced PGF2α and PGE2 secretion (P <0.01). TNF-α induced NO2/NO3 production by BOECs. The pretreatment of cells with E2 augmented only TNF-αnduced NO2/NO3 production (P <0.05). The aim of experiment 2 was to examine the influence of TNF-α, NO, and ovarian steroids on the protein content of enzymes specifically involved in PG and NO production, PG synthases, and NO synthases (NOSs). BOECs were treated with TNF-α (10 ng/mL) or NONOate (105 M). TNF-α increased the protein content of PGG/H synthase, PGF synthase, and PGE synthase (P <0.05) and endothelial and inducible NOSs (P <0.05). Nitric oxide increased the protein content of PGF synthase, PGE synthase, endothelial NOS, and inducible NOS (P <0.05). These results show possible linkage between TNF-α and NO, modulated by ovarian steroids, in the regulation of PG synthesis by BOECs that may be important for triggering the process of oviductal contractions.
| Original language | English |
|---|---|
| Pages (from-to) | 14-23 |
| Number of pages | 10 |
| Journal | Domestic Animal Endocrinology |
| Volume | 41 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jul 2011 |
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Keywords
- Cattle
- Nitric oxide
- Oviduct
- Prostaglandins
- Tumor necrosis factor-α
ASJC Scopus subject areas
- Animal Science and Zoology
- Endocrinology
- Food Animals
Cite this
Ovarian steroids modulate tumor necrosis factor-α and nitric oxideregulated prostaglandin secretion by cultured bovine oviductal epithelial cells. / Szóstek, A. Z.; Siemieniuch, M. J.; Deptula, K.; Woclawek-Potocka, I.; Majewska, M.; Okuda, K.; Skarzynski, D. J.
In: Domestic Animal Endocrinology, Vol. 41, No. 1, 07.2011, p. 14-23.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Ovarian steroids modulate tumor necrosis factor-α and nitric oxideregulated prostaglandin secretion by cultured bovine oviductal epithelial cells
AU - Szóstek, A. Z.
AU - Siemieniuch, M. J.
AU - Deptula, K.
AU - Woclawek-Potocka, I.
AU - Majewska, M.
AU - Okuda, K.
AU - Skarzynski, D. J.
PY - 2011/7
Y1 - 2011/7
N2 - Ovarian steroids assure an optimum environment for the final maturation of oocytes, gamete transport, fertilization, and early embryonic development. The aim of experiment 1 was to examine the influence of ovarian steroids on tumor necrosis factor-α (TNF-α) or nitric oxide (NO)regulated prostaglandin (PG), and nitrite/nitrate (NO2/NO3) secretion by cultured bovine oviductal epithelial cells (BOECs). BOECs were pretreated with 17β-estradiol (E2; 109 M) and/or progesterone (P4; 107 M) for 24 h. For the next 24 h, BOECs were treated with TNF-α (10 ng/mL) or spermine nitric oxide complex (NONOate; 105 M). Prostaglandin F2α and PGE2 secretion was measured in medium by ELISA. The pretreatment of cells with P4 (progesterone), E2 (17 β-estradiol), or E2/P4 augmented TNF-αinduced PGF2α and PGE2 secretion (P <0.01). The pretreatment of cells with E2 or E2/P4 increased NONOate-induced PGF2α and PGE2 secretion (P <0.01). TNF-α induced NO2/NO3 production by BOECs. The pretreatment of cells with E2 augmented only TNF-αnduced NO2/NO3 production (P <0.05). The aim of experiment 2 was to examine the influence of TNF-α, NO, and ovarian steroids on the protein content of enzymes specifically involved in PG and NO production, PG synthases, and NO synthases (NOSs). BOECs were treated with TNF-α (10 ng/mL) or NONOate (105 M). TNF-α increased the protein content of PGG/H synthase, PGF synthase, and PGE synthase (P <0.05) and endothelial and inducible NOSs (P <0.05). Nitric oxide increased the protein content of PGF synthase, PGE synthase, endothelial NOS, and inducible NOS (P <0.05). These results show possible linkage between TNF-α and NO, modulated by ovarian steroids, in the regulation of PG synthesis by BOECs that may be important for triggering the process of oviductal contractions.
AB - Ovarian steroids assure an optimum environment for the final maturation of oocytes, gamete transport, fertilization, and early embryonic development. The aim of experiment 1 was to examine the influence of ovarian steroids on tumor necrosis factor-α (TNF-α) or nitric oxide (NO)regulated prostaglandin (PG), and nitrite/nitrate (NO2/NO3) secretion by cultured bovine oviductal epithelial cells (BOECs). BOECs were pretreated with 17β-estradiol (E2; 109 M) and/or progesterone (P4; 107 M) for 24 h. For the next 24 h, BOECs were treated with TNF-α (10 ng/mL) or spermine nitric oxide complex (NONOate; 105 M). Prostaglandin F2α and PGE2 secretion was measured in medium by ELISA. The pretreatment of cells with P4 (progesterone), E2 (17 β-estradiol), or E2/P4 augmented TNF-αinduced PGF2α and PGE2 secretion (P <0.01). The pretreatment of cells with E2 or E2/P4 increased NONOate-induced PGF2α and PGE2 secretion (P <0.01). TNF-α induced NO2/NO3 production by BOECs. The pretreatment of cells with E2 augmented only TNF-αnduced NO2/NO3 production (P <0.05). The aim of experiment 2 was to examine the influence of TNF-α, NO, and ovarian steroids on the protein content of enzymes specifically involved in PG and NO production, PG synthases, and NO synthases (NOSs). BOECs were treated with TNF-α (10 ng/mL) or NONOate (105 M). TNF-α increased the protein content of PGG/H synthase, PGF synthase, and PGE synthase (P <0.05) and endothelial and inducible NOSs (P <0.05). Nitric oxide increased the protein content of PGF synthase, PGE synthase, endothelial NOS, and inducible NOS (P <0.05). These results show possible linkage between TNF-α and NO, modulated by ovarian steroids, in the regulation of PG synthesis by BOECs that may be important for triggering the process of oviductal contractions.
KW - Cattle
KW - Nitric oxide
KW - Oviduct
KW - Prostaglandins
KW - Tumor necrosis factor-α
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UR - http://www.scopus.com/inward/citedby.url?scp=79957991391&partnerID=8YFLogxK
U2 - 10.1016/j.domaniend.2011.01.007
DO - 10.1016/j.domaniend.2011.01.007
M3 - Article
C2 - 21420267
AN - SCOPUS:79957991391
VL - 41
SP - 14
EP - 23
JO - Domestic Animal Endocrinology
JF - Domestic Animal Endocrinology
SN - 0739-7240
IS - 1
ER -