TY - JOUR
T1 - Ovarian steroids modulate tumor necrosis factor-α and nitric oxideregulated prostaglandin secretion by cultured bovine oviductal epithelial cells
AU - Szóstek, A. Z.
AU - Siemieniuch, M. J.
AU - Deptula, K.
AU - Woclawek-Potocka, I.
AU - Majewska, M.
AU - Okuda, K.
AU - Skarzynski, D. J.
N1 - Funding Information:
This research was supported by the Polish Academy of Sciences and a joint Polish-Japanese project under the PAS and JSPS agreement. The authors thank Dr K. Watanabe from Division of Applied Life Sciences, Graduate School of Integrated Sciences and Arts, University of East Asia, Shimonoseki, Yamaguchi, Japan, for antibody against PGFS. The authors thank Dainippon Pharmaceutical Co., Ltd., Osaka, Japan, for recombinant human TNF (HF-13), and Henryk Jablonski, Experimental Farm of Agricultural Biology of Polish Academy of Sciences in Baranowo, Poland, for the excellent cooperation and permission to use their animals in the study. The authors are grateful to Draminski Electronics in Agriculture (Olsztyn, Poland, www.draminski.com ) for their excellent cooperation and the possibility to test and use the USG scanner.
PY - 2011/7
Y1 - 2011/7
N2 - Ovarian steroids assure an optimum environment for the final maturation of oocytes, gamete transport, fertilization, and early embryonic development. The aim of experiment 1 was to examine the influence of ovarian steroids on tumor necrosis factor-α (TNF-α) or nitric oxide (NO)regulated prostaglandin (PG), and nitrite/nitrate (NO2/NO3) secretion by cultured bovine oviductal epithelial cells (BOECs). BOECs were pretreated with 17β-estradiol (E2; 109 M) and/or progesterone (P4; 107 M) for 24 h. For the next 24 h, BOECs were treated with TNF-α (10 ng/mL) or spermine nitric oxide complex (NONOate; 105 M). Prostaglandin F2α and PGE2 secretion was measured in medium by ELISA. The pretreatment of cells with P4 (progesterone), E2 (17 β-estradiol), or E2/P4 augmented TNF-αinduced PGF2α and PGE2 secretion (P < 0.01). The pretreatment of cells with E2 or E2/P4 increased NONOate-induced PGF2α and PGE2 secretion (P < 0.01). TNF-α induced NO2/NO3 production by BOECs. The pretreatment of cells with E2 augmented only TNF-αnduced NO2/NO3 production (P < 0.05). The aim of experiment 2 was to examine the influence of TNF-α, NO, and ovarian steroids on the protein content of enzymes specifically involved in PG and NO production, PG synthases, and NO synthases (NOSs). BOECs were treated with TNF-α (10 ng/mL) or NONOate (105 M). TNF-α increased the protein content of PGG/H synthase, PGF synthase, and PGE synthase (P < 0.05) and endothelial and inducible NOSs (P < 0.05). Nitric oxide increased the protein content of PGF synthase, PGE synthase, endothelial NOS, and inducible NOS (P < 0.05). These results show possible linkage between TNF-α and NO, modulated by ovarian steroids, in the regulation of PG synthesis by BOECs that may be important for triggering the process of oviductal contractions.
AB - Ovarian steroids assure an optimum environment for the final maturation of oocytes, gamete transport, fertilization, and early embryonic development. The aim of experiment 1 was to examine the influence of ovarian steroids on tumor necrosis factor-α (TNF-α) or nitric oxide (NO)regulated prostaglandin (PG), and nitrite/nitrate (NO2/NO3) secretion by cultured bovine oviductal epithelial cells (BOECs). BOECs were pretreated with 17β-estradiol (E2; 109 M) and/or progesterone (P4; 107 M) for 24 h. For the next 24 h, BOECs were treated with TNF-α (10 ng/mL) or spermine nitric oxide complex (NONOate; 105 M). Prostaglandin F2α and PGE2 secretion was measured in medium by ELISA. The pretreatment of cells with P4 (progesterone), E2 (17 β-estradiol), or E2/P4 augmented TNF-αinduced PGF2α and PGE2 secretion (P < 0.01). The pretreatment of cells with E2 or E2/P4 increased NONOate-induced PGF2α and PGE2 secretion (P < 0.01). TNF-α induced NO2/NO3 production by BOECs. The pretreatment of cells with E2 augmented only TNF-αnduced NO2/NO3 production (P < 0.05). The aim of experiment 2 was to examine the influence of TNF-α, NO, and ovarian steroids on the protein content of enzymes specifically involved in PG and NO production, PG synthases, and NO synthases (NOSs). BOECs were treated with TNF-α (10 ng/mL) or NONOate (105 M). TNF-α increased the protein content of PGG/H synthase, PGF synthase, and PGE synthase (P < 0.05) and endothelial and inducible NOSs (P < 0.05). Nitric oxide increased the protein content of PGF synthase, PGE synthase, endothelial NOS, and inducible NOS (P < 0.05). These results show possible linkage between TNF-α and NO, modulated by ovarian steroids, in the regulation of PG synthesis by BOECs that may be important for triggering the process of oviductal contractions.
KW - Cattle
KW - Nitric oxide
KW - Oviduct
KW - Prostaglandins
KW - Tumor necrosis factor-α
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U2 - 10.1016/j.domaniend.2011.01.007
DO - 10.1016/j.domaniend.2011.01.007
M3 - Article
C2 - 21420267
AN - SCOPUS:79957991391
VL - 41
SP - 14
EP - 23
JO - Domestic Animal Endocrinology
JF - Domestic Animal Endocrinology
SN - 0739-7240
IS - 1
ER -