Outer membrane vesicles of porphyromonas gingivalis elicit a mucosal immune response

Ryoma Nakao, Hideki Hasegawa, Kuniyasu Ochiai, Shogo Takashiba, Akira Ainai, Makoto Ohnishi, Haruo Watanabe, Hidenobu Senpuku

Research output: Contribution to journalArticle

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Abstract

We previously reported that mutation of galE in Porphyromonas gingivalis has pleiotropic effects, including a truncated lipopolysaccharide (LPS) O-antigen and deglycosylation of the outer membrane protein OMP85 homolog. In the present study, further analysis of the galE mutant revealed that it produced little or no outer membrane vesicles (OMVs). Using three mouse antisera raised against whole cells of the P. gingivalis wild type strain, we performed ELISAs to examine the reactivity of these antisera with whole cells of the wild type or the galE mutant. All three antisera had significantly lower reactivity against the galE mutant compared to wild type. OMVs, but not LPS, retained the immunodominant determinant of P. gingivalis, as determined by ELISAs (with wild type LPS or OMVs as antigen) and absorption assays. In addition, we assessed the capacity of OMVs as a vaccine antigen by intranasal immunization to BALB/c mice. Synthetic double-stranded RNA polyriboinosinic polyribocytidylic acid [Poly (I:C)], an agonist of Toll-like receptor 3 (TLR3), was used as the mucosal adjuvant. Vaccination with OMV elicited dramatically high levels of P. gingivalis-specific IgA in nasal washes and saliva, as well as serum IgG and IgA. In conclusion, the OMVs of P. gingivalis have an important role in mucosal immunogenicity as well as in antigenicity. We propose that P. gingivalis OMV is an intriguing immunogen for development of a periodontal disease vaccine.

Original languageEnglish
Article numbere26163
JournalPLoS One
Volume6
Issue number10
DOIs
Publication statusPublished - 2011

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Porphyromonas gingivalis
mucosal immunity
Mucosal Immunity
Membranes
lipopolysaccharides
antigens
antiserum
Lipopolysaccharides
Immune Sera
mutants
Immunoglobulin A
enzyme-linked immunosorbent assay
vaccines
Vaccines
Enzyme-Linked Immunosorbent Assay
Toll-Like Receptor 3
Immunization
outer membrane proteins
Antigens
double-stranded RNA

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Nakao, R., Hasegawa, H., Ochiai, K., Takashiba, S., Ainai, A., Ohnishi, M., ... Senpuku, H. (2011). Outer membrane vesicles of porphyromonas gingivalis elicit a mucosal immune response. PLoS One, 6(10), [e26163]. https://doi.org/10.1371/journal.pone.0026163

Outer membrane vesicles of porphyromonas gingivalis elicit a mucosal immune response. / Nakao, Ryoma; Hasegawa, Hideki; Ochiai, Kuniyasu; Takashiba, Shogo; Ainai, Akira; Ohnishi, Makoto; Watanabe, Haruo; Senpuku, Hidenobu.

In: PLoS One, Vol. 6, No. 10, e26163, 2011.

Research output: Contribution to journalArticle

Nakao, R, Hasegawa, H, Ochiai, K, Takashiba, S, Ainai, A, Ohnishi, M, Watanabe, H & Senpuku, H 2011, 'Outer membrane vesicles of porphyromonas gingivalis elicit a mucosal immune response', PLoS One, vol. 6, no. 10, e26163. https://doi.org/10.1371/journal.pone.0026163
Nakao, Ryoma ; Hasegawa, Hideki ; Ochiai, Kuniyasu ; Takashiba, Shogo ; Ainai, Akira ; Ohnishi, Makoto ; Watanabe, Haruo ; Senpuku, Hidenobu. / Outer membrane vesicles of porphyromonas gingivalis elicit a mucosal immune response. In: PLoS One. 2011 ; Vol. 6, No. 10.
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