Outer capsid protein heterogeneity of rice dwarf phytoreovirus

Nobuhiro Suzuki, M. Sugawara

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The 46K outer capsid protein encoded by RNA segment S8 and the 42K polypeptide, previously thought to be the segment S9-encoded structural protein, were isolated from a rice dwarf phytoreovirus purified preparation, and then analysed by peptide mapping and electroblot-ELlSA. Staphylococcus aureus V8 protease peptide mapping patterns of the 42K and 46K proteins were similar. Two monoclonal antibodies (MAbs), obtained after immunization with virus particles dissociated by 0.1% SDS, were each specific for both the 42K and 46K proteins. Furthermore, the MAbs bound common peptide fragments which were generated by digestion of the 42K and 46K proteins with V8 protease or proteinase K. These results strongly suggest that the 42K protein is not a gene product of S9 but a product overlapping with the 46K outer capsid protein. Whether the two proteins are functionally distinct remains to be determined.

Original languageEnglish
Pages (from-to)2239-2242
Number of pages4
JournalJournal of General Virology
Volume72
Issue number9
Publication statusPublished - 1991
Externally publishedYes

Fingerprint

Reoviridae
Capsid Proteins
Endopeptidase K
Proteins
Peptide Mapping
Monoclonal Antibodies
Peptide Fragments
Virion
Staphylococcus aureus
Oryza
Digestion
Immunization
RNA
Peptides
Genes

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Outer capsid protein heterogeneity of rice dwarf phytoreovirus. / Suzuki, Nobuhiro; Sugawara, M.

In: Journal of General Virology, Vol. 72, No. 9, 1991, p. 2239-2242.

Research output: Contribution to journalArticle

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