OsYSl6 is involved in the detoxification of excess manganese in rice

Akimasa Sasaki, Naoki Yamaji, Jixing Xia, Jian Feng Ma

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Yellow Stripe-Like (YSL) proteins belong to the oligopeptide transporter family and have been implicated in metal transport and homeostasis in different plant species. Here, we functionally characterized a rice (Oryza sativa) YSL member, OsYSL6. Knockout of OsYSL6 resulted in decreased growth of both roots and shoots only in the high-manganese (Mn) condition. There was no difference in the concentration of total Mn and other essential metals between the wild-type rice and the knockout line, but the knockout line showed a higher Mn concentration in the leaf apoplastic solution and a lower Mn concentration in the symplastic solution than wild-type rice. OsYSL6 was constitutively expressed in both the shoots and roots, and the expression level was not affected by either deficiency or toxicity of various metals. Furthermore, the expression level increased with leaf age. Analysis with OsYSL6 promoter-green fluorescent protein transgenic rice revealed that OsYSL6 was expressed in all cells of both the roots and shoots. Heterogolous expression of OsYSL6 in yeast showed transport activity for the Mn-nicotianamine complex but not for the Mn-mugineic acid complex. Taken together, our results suggest that OsYSL6 is a Mn-nicotianamine transporter that is required for the detoxification of excess Mn in rice.

Original languageEnglish
Pages (from-to)1832-1840
Number of pages9
JournalPlant Physiology
Volume157
Issue number4
DOIs
Publication statusPublished - Dec 2011

Fingerprint

Manganese
manganese
rice
Metals
metals
shoots
transporters
oligopeptides
Oligopeptides
Oryza
Green Fluorescent Proteins
green fluorescent protein
leaves
root growth
homeostasis
Oryza sativa
Homeostasis
Yeasts
promoter regions
genetically modified organisms

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

Cite this

OsYSl6 is involved in the detoxification of excess manganese in rice. / Sasaki, Akimasa; Yamaji, Naoki; Xia, Jixing; Ma, Jian Feng.

In: Plant Physiology, Vol. 157, No. 4, 12.2011, p. 1832-1840.

Research output: Contribution to journalArticle

Sasaki, Akimasa ; Yamaji, Naoki ; Xia, Jixing ; Ma, Jian Feng. / OsYSl6 is involved in the detoxification of excess manganese in rice. In: Plant Physiology. 2011 ; Vol. 157, No. 4. pp. 1832-1840.
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