OsYSL16 is required for preferential Cu distribution to floral organs in rice

Chang Zhang, Wenhui Lu, Yang Yang, Zhenguo Shen, Jian Feng Ma, Luqing Zheng

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Deficiency of copper (Cu) causes low fertility in many plant species, but the molecular mechanisms underlying distribution of Cu to the floral organs are poorly understood. Here, we found that a member of yellow-stripe like (YSL) family, YSL16 encoding the Cu-nicotianamine (Cu-NA) transporter, was highly expressed in the rachilla, with less expression in the palea and lemma of rice (Oryza sativa). β-Glucuronidase (GUS) staining of transgenic rice carrying the OsYSL16 promoter-GUS showed that OsYSL16 was mainly expressed in vascular bundles of the rachilla as well as the palea and lemma. Knockout of OsYSL16 resulted in decreased Cu distribution to the stamens, but increased distribution to the palea and lemma. A short-term (24 h) 65 Cu labeling experiment confirmed increased Cu concentration of palea and lemma in the mutant. Furthermore, we found that redistribution of Cu from the palea and lemma was impaired in the osysl16 mutant after exposure to Cu-free solution. The osysl16 mutant showed low pollen germination, but this was rescued by addition of Cu in the medium. Our results indicate that OsYSL16 expressed in the vascular bundles of the rachilla is important for preferential distribution of Cu to the stamens, while OsYSL16 in vascular bundles of the palea and lemma is involved in Cu redistribution under Cu-limited conditions in rice.

Original languageEnglish
Pages (from-to)2039-2051
Number of pages13
JournalPlant and Cell Physiology
Volume59
Issue number10
DOIs
Publication statusPublished - Oct 1 2018

Fingerprint

vascular bundles
stamens
Blood Vessels
rice
mutants
Glucuronidase
pollen germination
transporters
Oryza sativa
Germination
Pollen
copper
promoter regions
genetically modified organisms
Fertility
Copper
Staining and Labeling
Oryza

Keywords

  • Copper distribution
  • Floral organs
  • OsYSL16
  • Pollen germination
  • Rice

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

Cite this

OsYSL16 is required for preferential Cu distribution to floral organs in rice. / Zhang, Chang; Lu, Wenhui; Yang, Yang; Shen, Zhenguo; Ma, Jian Feng; Zheng, Luqing.

In: Plant and Cell Physiology, Vol. 59, No. 10, 01.10.2018, p. 2039-2051.

Research output: Contribution to journalArticle

Zhang, Chang ; Lu, Wenhui ; Yang, Yang ; Shen, Zhenguo ; Ma, Jian Feng ; Zheng, Luqing. / OsYSL16 is required for preferential Cu distribution to floral organs in rice. In: Plant and Cell Physiology. 2018 ; Vol. 59, No. 10. pp. 2039-2051.
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AB - Deficiency of copper (Cu) causes low fertility in many plant species, but the molecular mechanisms underlying distribution of Cu to the floral organs are poorly understood. Here, we found that a member of yellow-stripe like (YSL) family, YSL16 encoding the Cu-nicotianamine (Cu-NA) transporter, was highly expressed in the rachilla, with less expression in the palea and lemma of rice (Oryza sativa). β-Glucuronidase (GUS) staining of transgenic rice carrying the OsYSL16 promoter-GUS showed that OsYSL16 was mainly expressed in vascular bundles of the rachilla as well as the palea and lemma. Knockout of OsYSL16 resulted in decreased Cu distribution to the stamens, but increased distribution to the palea and lemma. A short-term (24 h) 65 Cu labeling experiment confirmed increased Cu concentration of palea and lemma in the mutant. Furthermore, we found that redistribution of Cu from the palea and lemma was impaired in the osysl16 mutant after exposure to Cu-free solution. The osysl16 mutant showed low pollen germination, but this was rescued by addition of Cu in the medium. Our results indicate that OsYSL16 expressed in the vascular bundles of the rachilla is important for preferential distribution of Cu to the stamens, while OsYSL16 in vascular bundles of the palea and lemma is involved in Cu redistribution under Cu-limited conditions in rice.

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